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Department of Physiology, University of Tennessee Health Science Center, Memphis, Tennessee 38163
The
febrile responses of splenectomized (Splex) or sham-operated (Sham)
guinea pigs challenged intravenously or intraperitoneally with
lipopolysaccharide (LPS) 7 and 30 days after surgery were evaluated.
FITC-LPS uptake by Kupffer cells (KC) was additionally assessed 15, 30, and 60 min after injection. LPS at 0.05 µg/kg iv did not evoke fever
in Sham animals but caused a 1.2°C core temperature (Tc)
rise in the Splex animals. LPS at 2 µg/kg iv induced a 1.8°C
greater Tc rise of the Splex animals than of their controls. LPS at 2 and 8 µg/kg ip 7 days postsurgery induced 1.4 and
1.8°C higher fevers, respectively, in the Splex than Sham animals.
LPS at 2 and 8 µg/kg ip 30 days postsurgery also increased the
febrile responses of the asplenic animals by 1.6 and 1.8°C, respectively. FITC-LPS at 7 days was detected in the controls within KC
15 min after its administration; the label density was reduced at 30 min and almost 0 at 60 min. In the Splex group, in contrast, the
labeling was significantly denser and remained unchanged through all
three time points; this effect was still present 30 days after surgery.
Similar results were obtained at 60 min after FITC-LPS intraperitoneal
injection. Gadolinium chloride pretreatment (
3 days) of the Splex
group significantly reduced both their febrile responses to LPS (8 µg/kg ip) and their KC uptake of FITC-LPS 7 days postsurgery. Thus
splenectomy increases the magnitude of the febrile response of guinea
pigs and the uptake of systemically administered LPS.
fever; splenectomy; Kupffer cells; fluorescein isothiocyanate-lipopolysaccharide
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