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LOCAL CONTROL OF CIRCULATION
1Copenhagen Muscle Research Center, Institute of Exercise and Sport Sciences, University of Copenhagen, 2100 Copenhagen; and 2Sports Medicine Research Unit, Copenhagen University Hospital, 2400 Copenhagen, Denmark
Submitted 21 January 2003 ; accepted in final form 21 March 2003
Interstitial K+ ([K+]i) was measured in human skeletal muscle by microdialysis during exhaustive leg exercise, with (AL) and without (L) previous intense arm exercise. In addition, the reproducibility of the [K+]i determinations was
examined. Possible microdialysis-induced rupture of the sarcolemma was
assessed by measurement of carnosine in the dialysate, because carnosine is
only expected to be found intracellularly. Changes in
[K+]i could be reproduced, when exhaustive leg exercise
was performed on two different days, with a between-day difference of 
0.5
mM at rest and 1.5 mM at exhaustion. The time to exhaustion was shorter in AL
than in L (2.7 ± 0.3 vs. 4.0 ± 0.3 min; P < 0.05).
Furthermore, [K+]i was higher from 0 to 1.5 min of the
intense leg exercise period in AL compared with L (9.2 ± 0.7 vs. 6.4
± 0.9 mM; P < 0.001) and at exhaustion (11.9 ± 0.5
vs. 10.3 ± 0.6 mM; P < 0.05). The dialysate content of
carnosine was elevated by exercise, but low-intensity exercise resulted in
higher dialysate carnosine concentrations than subsequent intense exercise.
Furthermore, no relationship was found between carnosine concentrations and
[K+]i. Thus the present data suggest that microdialysis
can be used to determine muscle [K+]i kinetics during
intense exercise, when low-intensity exercise is performed before the intense
exercise. The high [K+]i levels reached at exhaustion
can be expected to cause fatigue, which is supported by the finding that a
faster accumulation of interstitial K+, induced by prior arm
exercise, was associated with a reduced time to fatigue.
muscle; fatigue; microdialysis; exercise; pH; potassium
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