HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 285/3/R601    most recent 00419.2002v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (6) Citing Articles via Google Scholar Google Scholar Articles by Chugun, A. Articles by Akera, T. Search for Related Content PubMed PubMed Citation Articles by Chugun, A. Articles by Akera, T.

CARDIAC, RENAL, AND RESPIRATORY INTEGRATION

Subcellular distribution of ryanodine receptors in the cardiac muscle of carp (Cyprinus carpio)

Departments of ¹Veterinary Pharmacology, ³Veterinary Anatomy, and ⁴Toxicology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada-shi, Aomori 034-8628; Department of ²Pharmacology, Juntendo University, School of Medicine, Tokyo 113-8421; and ⁵Merck Sharp and Dohme Research Laboratories Japan, 9-20, Akasaka, 1-chome, Minato-ku, Tokyo 107-0052, Japan

Submitted 16 July 2002 ; accepted in final form 5 June 2003

We examined the subcellular localization of ryanodine receptors (RyR) in the cardiac muscle of carp using biochemical, immunohistochemical, and electron microscopic methods and compared it with those of rats and guinea pigs. To achieve this goal, an anti-RyR antibody was newly raised against a synthetic peptide corresponding to an amino acid sequence that was conserved among all sequenced RyRs. Western blot analysis using this antibody detected a single RyR band following the SDS-PAGE of sarcoplasmic reticulum (SR) membranes from carp atrium and ventricle as well as from mammalian hearts and skeletal muscles. The carp heart band had slightly greater mobility than those of mammalian hearts. Although immunohistochemical staining showed evident striations corresponding to the Z lines in longitudinal sections of mammalian hearts, clusters of punctate staining, in contrast, were distributed ubiquitously throughout carp atrium and ventricle. Electron microscopic images of the carp myocardium showed that the SR was observed largely as the subsarcolemmal cisternae and the reticular SR, suggesting that the RyR is localized in the junctional and corbular SR.

anti-ryanodine receptor antibody; immunolocalization; sarcoplasmic reticulum

This article has been cited by other articles:

				A. Chugun, O. Sato, H. Takeshima, and Y. Ogawa Mg2+ activates the ryanodine receptor type 2 (RyR2) at intermediate Ca2+ concentrations Am J Physiol Cell Physiol, January 1, 2007; 292(1): C535 - C544. [Abstract] [Full Text] [PDF]

				H. A. Shiels and E. White Temporal and spatial properties of cellular Ca2+ flux in trout ventricular myocytes Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2005; 288(6): R1756 - R1766. [Abstract] [Full Text] [PDF]

				T. Murayama and Y. Ogawa RyR1 exhibits lower gain of CICR activity than RyR3 in the SR: evidence for selective stabilization of RyR1 channel Am J Physiol Cell Physiol, July 1, 2004; 287(1): C36 - C45. [Abstract] [Full Text] [PDF]