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INVITED REVIEW
1Department of Anesthesiology and the 2Center for Free Radical Biology, University of Alabama at Birmingham, Birmingham, Alabama 35294; 3Radiation Biology Branch, National Cancer Institute, Bethesda, Maryland 20814; and 4Department of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center, Shreveport, Louisiana 71130
Facile detection of reactive oxygen and nitrogen species in biologic systems is often problematic. This is a result of the numerous cellular mechanisms, both enzymatic and nonenzymatic involved in their catabolism/decomposition, the complex and overlapping nature of their reactivities, as well as the often limited intracellular access of detector systems. This review describes approaches to the direct and indirect measurement of different reactive metabolites of oxygen and nitrogen. Particular attention to a method's applicability for in vivo determinations will be addressed.
free radical; nitric oxide; superoxide; hydrogen peroxide; peroxynitrite
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