HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 286/3/R591    most recent 00559.2003v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (2) Citing Articles via Google Scholar Google Scholar Articles by Tsuchiya, Y. Articles by Morita, H. Search for Related Content PubMed PubMed Citation Articles by Tsuchiya, Y. Articles by Morita, H.

THIRST AND VOLUME, ELECTROLYTE HOMEOSTASIS

Effect of high-NaCl or high-KCl diet on hepatic Na⁺- and K⁺-receptor sensitivity and NKCC1 expression in rats

¹Department of Physiology and ³Department of Biochemistry, Gifu University School of Medicine, Gifu 500-8705; and ²Department of Food and Nutritional Sciences, Shizuoka Prefectural University, Shizuoka 422-8526, Japan

Submitted 26 September 2003 ; accepted in final form 27 November 2003

We previously reported that the bumetanide-sensitive Na⁺-K⁺-2Cl^- cotransporter (NKCC1) is involved in the hepatic Na⁺ and K⁺ sensor mechanism. In the present study, we examined the effects of a high-NaCl or high-KCl diet on hepatic Na⁺ and K⁺ receptor sensitivity and NKCC1 expression in the liver of Sprague-Dawley rats. RT-PCR and Western blots were used to measure NKCC1 mRNA and protein expression, respectively. Infusion of hypertonic NaCl or isotonic KCl + NaCl solutions into the portal vein increased hepatic afferent nerve activity (HANA) in a Na⁺ or K⁺ dose-dependent manner. After 4 wk on a high-NaCl or high-KCl diet, HANA responses were attenuated compared with animals fed a normal diet, and NKCC1 expression was reduced. These results show that a high-NaCl or high-KCl diet decreases NKCC1 expression in the liver, and it might cause a reduction in hepatic Na⁺- and K⁺-receptor sensitivity.

sodium-potassium-2 chloride cotransporter; hepatic afferent nerve activity; portal venous injection; hypertonic sodium chloride

This article has been cited by other articles:

				S. N. Orlov and A. A. Mongin Salt-sensing mechanisms in blood pressure regulation and hypertension Am J Physiol Heart Circ Physiol, October 1, 2007; 293(4): H2039 - H2053. [Abstract] [Full Text] [PDF]