| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
LOCAL CONTROL OF CIRCULATION
1Department of Biological Sciences and 2South Bend Center for Medical Education, Indiana University School of Medicine, University of Notre Dame, Notre Dame, Indiana 46556
Submitted 25 July 2003 ; accepted in final form 15 December 2003
Hydrogen sulfide (H2S) is an endogenous vasodilator in mammals, but its presence and function in other vertebrates is unknown. We generated H2S from NaHS and examined the effects on isolated efferent branchial arteries from steelhead (stEBA) or rainbow (rtEBA) trout. H2S concentration was measured colorimetrically (CM) and with ion-selective electrodes (ISE) in rainbow trout plasma. NaHS produced a triphasic response consisting of a relaxation (phase 1), constriction (phase 2), and relaxation (phase 3) in both unstimulated vessels and in stEBA precontracted with carbachol (Carb). Phase 1 and phase 3 in stEBA were decreased and phase 2 increased in unstimulated vessels by K+ATP channel inhibition (glibenclamide), or a cocktail of inhibitors of cyclooxygenase, lipoxygenase, and cytochrome P-450 (indomethacin, esculetin, and clotrimazole). Inhibition of soluble guanylate cyclase with ODQ or NS-2028 inhibited phase 3 in stEBA, although NaHS decreased cGMP production by rtEBA. stEBA phase 2 contractions were partially inhibited by the myosin light chain kinase inhibitor, ML-9, but unaffected by L-type calcium channel inhibition (methoxyverapamil), whereas contraction in rtEBA was partially inhibited by nifedipine or removal of extracellular calcium. Phase 3 relaxations were more pronounced in stEBA precontracted with Carb and norepinephrine (NE) than those contracted by KCl or K2SO4. stEBA phase 2 and phase 3 responses were dose dependent (EC50 = 1.1 ± 1.2 x 10-3 M and 6.7 ± 0.9 x 10-5 M, respectively; n = 7). NaHS was also vasoactive in steelhead bulbus arteriosus, celiacomesenteric arteries, and anterior cardinal veins. Rainbow trout plasma sulfide concentration was 4.0 ± 0.3 x 10-5 M, n = 4 (CM) and 3.8 ± 0.4 x 10-5 M, n = 9 (ISE); similar to phase 3 EC50. Because NaHS has substantial vasoactive effects at physiological plasma concentrations, we propose that its soluble derivative, H2S, is a tonically active endogenous vasoregulator in trout.
fish; cardiovascular; guanosine 3',5'-cyclic monophosphate; vasodilator; vasoconstrictor
This article has been cited by other articles:
|
|
 |
|
  M Matsunami, T Tarui, K Mitani, K Nagasawa, O Fukushima, K Okubo, S Yoshida, M Takemura, and A Kawabata Luminal hydrogen sulfide plays a pronociceptive role in mouse colon Gut, June 1, 2009; 58(6): 751 - 761. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. F. Perry, B. McNeill, E. Elia, A. Nagpal, and B. Vulesevic Hydrogen sulfide stimulates catecholamine secretion in rainbow trout (Oncorhynchus mykiss) Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2009; 296(1): R133 - R140. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Dombkowski, N. L. Whitfield, R. Motterlini, Y. Gao, and K. R. Olson Effects of carbon monoxide on trout and lamprey vessels Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2009; 296(1): R141 - R149. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. R. Olson Hydrogen sulfide and oxygen sensing: implications in cardiorespiratory control J. Exp. Biol., September 1, 2008; 211(17): 2727 - 2734. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. C. Yong, S. W. Lee, C. S. Foo, K. L. Neo, X. Chen, and J.-S. Bian Endogenous hydrogen sulphide mediates the cardioprotection induced by ischemic postconditioning Am J Physiol Heart Circ Physiol, September 1, 2008; 295(3): H1330 - H1340. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. D. Webb, L. H. Lim, V. M. S. Oh, S. B. Yeo, Y. P. Cheong, M. Y. Ali, R. El Oakley, C. N. Lee, P. S. Wong, M. G. Caleb, et al. Contractile and Vasorelaxant Effects of Hydrogen Sulfide and Its Biosynthesis in the Human Internal Mammary Artery J. Pharmacol. Exp. Ther., February 1, 2008; 324(2): 876 - 882. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Qu, C. P.L.H. Chen, B. Halliwell, P. K. Moore, and P. T.-H. Wong Hydrogen Sulfide Is a Mediator of Cerebral Ischemic Damage Stroke, March 1, 2006; 37(3): 889 - 893. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Julian, J. Statile, T. A. Roepke, and A. J. Arp Sodium Nitroprusside Potentiates Hydrogen-Sulfide-Induced Contractions in Body Wall Muscle From a Marine Worm Biol. Bull., August 1, 2005; 209(1): 6 - 10. [Full Text] [PDF]
|
|
|
|
|
|
L. F. Gainey Jr. and M. J. Greenberg Hydrogen Sulfide Is Synthesized in the Gills of the Clam Mercenaria mercenaria and Acts Seasonally to Modulate Branchial Muscle Contraction Biol. Bull., August 1, 2005; 209(1): 11 - 20. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Dombkowski, M. J. Russell, A. A. Schulman, M. M. Doellman, and K. R. Olson Vertebrate phylogeny of hydrogen sulfide vasoactivity Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2005; 288(1): R243 - R252. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
