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LOCAL CONTROL OF CIRCULATION

Expression of adrenomedullin in hypoxic and ischemic rat kidneys and human kidneys with arterial stenosis

¹Pharmaforschungszentrum, Bayer AG, D-42096 Wuppertal; ²Institut für Physiologie, Universität Regensburg, D-93040 Regensburg, Germany; ³Department of Physiology and Pharmacology, University of Southern Denmark, DK-5000 Odense; and ⁴Institute of Pathology and ⁵Department of Urology, Odense University Hospital, DK-5000 Odense, Denmark

Submitted 19 May 2003 ; accepted in final form 4 January 2004

To investigate regional aspects of hypoxic regulation of adrenomedullin (AM) in kidneys, we mapped the distribution of AM in the rat kidney after hypoxia (normobaric hypoxic hypoxia, carbon monoxide, and CoCl₂ for 6 h), anemia (hematocrit lowered by bleeding) and after global transient ischemia for 1 h (unilateral renal artery occlusion and reperfusion for 6 and 24 h) and segmental infarct (6 and 24 h). AM expression and localization was determined in normal human kidneys and in kidneys with arterial stenosis. Hypoxia stimulated AM mRNA expression significantly in rat inner medulla (CO 13 times, 8% O₂ 6 times, and CoCl₂ 8 times), followed by the outer medulla and cortex. AM mRNA level was significantly elevated in response to anemia and occlusion-reperfusion. Immunoreactive AM was associated with the thin limbs of Henle's loop, distal convoluted tubule, collecting ducts, papilla surface epithelium, and urothelium. AM labeling was prominent in the inner medulla after CO and in the outer medulla after occlusion-reperfusion. The infarct border zone was strongly labeled for AM. In cultured inner medullary collecting duct cells, AM mRNA was significantly increased by hypoxia. AM mRNA was equally distributed in human kidney and AM was localized as in the rat kidney. In human kidneys with artery stenosis, AM mRNA was not significantly enhanced compared with controls, but AM immunoreactivity was observed in tubules, vessels, and glomerular cells. In summary, AM expression was increased in the rat kidney in response to hypoxic and ischemic hypoxia in keeping with oxygen gradients. AM was widely distributed in the human kidney with arterial stenosis. AM may play a significant role to counteract hypoxia in the kidney.

anemia; hypertension; infarct; oxygen

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