AJP - Regu AJP: Advances in Physiology Education
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Regul Integr Comp Physiol 287: R218-R227, 2004. First published March 4, 2004; doi:10.1152/ajpregu.00377.2003
0363-6119/04 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
287/1/R218    most recent
00377.2003v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Commerford, S. R.
Right arrow Articles by O'Doherty, R. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Commerford, S. R.
Right arrow Articles by O'Doherty, R. M.

APPETITE, OBESITY AND METABOLISM

In vivo regulation of SREBP-1c in skeletal muscle: effects of nutritional status, glucose, insulin, and leptin

S. Renee Commerford, Li Peng, John J. Dubé, and Robert M. O'Doherty

Department of Medicine, Division of Endocrinology, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15261

Submitted 9 July 2003 ; accepted in final form 16 February 2004

Sterol regulatory element binding protein-1c (SREBP-1c), a transcription factor that is important for mediating insulin effects on metabolic gene expression in liver during the fasted-to-fed transition, is also expressed in skeletal muscle. However, the regulation and role of SREBP-1c in skeletal muscle are poorly understood. The present study compared the effects of nutritional status, physiological hyperinsulinemic clamps, and adenovirus-mediated hyperleptinemia (HLEP) in rats on expression of SREBP-1c and other metabolic genes in skeletal muscle. Three- and 6-h refeeding of 18-h-fasted animals increased levels of SREBP-1c mRNA and the SREBP-1 protein (full length and mature) in gastrocnemius muscle (P < 0.05). Fatty acid synthase (FAS) and hexokinase II (HKII) mRNA levels were also increased by refeeding, and uncoupling protein 3 (UCP3) mRNA level was decreased (all P < 0.05). Surprisingly, 3-h hyperinsulinemic clamps did not increase gastrocnemius muscle SREBP-1c and FAS mRNA levels or SREBP-1 protein levels but did increase HKII mRNA levels and decrease UCP3 mRNA levels (P < 0.05). HLEP reduced refeeding-induced increases of SREBP-1c and FAS mRNA levels but did not reduce the level of SREBP-1 protein. We conclude that 1) skeletal muscle SREBP-1c gene expression is regulated by nutritional status in a fashion similar to that observed in liver and adipose tissue, 2) physiological hyperinsulinemia is not sufficient to imitate the effects of refeeding on SREBP-1c gene expression, and 3) leptin suppresses refeeding effects on SREBP-1c mRNA levels.

nutrient partitioning; lipids; gene expression


Address for reprint requests and other correspondence: R. M. O'Doherty, 200 Lothrop St., BST-E1140, Pittsburgh, PA 15261 (Odohertyr{at}msx.dept-med.pitt.edu).




This article has been cited by other articles:


Home page
 ptjournalHome page
L. P Turcotte and J. S Fisher
Skeletal Muscle Insulin Resistance: Roles of Fatty Acid Metabolism and Exercise
Physical Therapy, November 1, 2008; 88(11): 1279 - 1296.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
J. de Wilde, R. Mohren, S. van den Berg, M. Boekschoten, K. W.-V. Dijk, P. de Groot, M. Muller, E. Mariman, and E. Smit
Short-term high fat-feeding results in morphological and metabolic adaptations in the skeletal muscle of C57BL/6J mice
Physiol Genomics, February 19, 2008; 32(3): 360 - 369.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
J. J. Dube, B. A. Bhatt, N. Dedousis, A. Bonen, and R. M. O'Doherty
Leptin, skeletal muscle lipids, and lipid-induced insulin resistance
Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2007; 293(2): R642 - R650.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
K. Tsintzas, K. Jewell, M. Kamran, D. Laithwaite, T. Boonsong, J. Littlewood, I. Macdonald, and A. Bennett
Differential regulation of metabolic genes in skeletal muscle during starvation and refeeding in humans
J. Physiol., August 15, 2006; 575(1): 291 - 303.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
K. J. Nadeau, L. B. Ehlers, L. E. Aguirre, R. L. Moore, K. N. Jew, H. K. Ortmeyer, B. C. Hansen, J. E. B. Reusch, and B. Draznin
Exercise training and calorie restriction increase SREBP-1 expression and intramuscular triglyceride in skeletal muscle
Am J Physiol Endocrinol Metab, July 1, 2006; 291(1): E90 - E98.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
B. A. Bhatt, J. J. Dube, N. Dedousis, J. A. Reider, and R. M. O'Doherty
Diet-induced obesity and acute hyperlipidemia reduce I{kappa}B{alpha} levels in rat skeletal muscle in a fiber-type dependent manner
Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2006; 290(1): R233 - R240.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
P. S. MacLean
A peripheral perspective of weight regain
Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2005; 288(6): R1447 - R1449.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2004 by the American Physiological Society.