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ENVIRONMENTAL, EXERCISE AND RESPIRATORY PHYSIOLOGY

Effect of acute exercise and exercise training on VEGF splice variants in human skeletal muscle

¹Institute for Exercise and Sport Science; and ²The August Krogh Institute, Copenhagen Muscle Research Centre, Copenhagen University, 2100 Copenhagen, Denmark; and ³The John B. Pierce Laboratory and the Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06519

Submitted 3 February 2004 ; accepted in final form 28 April 2004

The present study investigated the effect of an acute exercise bout on the mRNA response of vascular endothelial growth factor (VEGF) splice variants in untrained and trained human skeletal muscle. Seven habitually active young men performed one-legged knee-extensor exercise training at an intensity corresponding to 70% of the maximal workload in an incremental test five times/week for 4 wk. Biopsies were obtained from the vastus lateralis muscle of the trained and untrained leg 40 h after the last training session. The subjects then performed 3 h of two-legged knee-extensor exercise, and biopsies were obtained from both legs after 0, 2, 6, and 24 h of recovery. Real-time PCR was used to examine the expression of VEGF mRNA containing exon 1 and 2 (all VEGF isoforms), exon 6 or exon 7, and VEGF₁₆₅ mRNA. Acute exercise induced an increase (P < 0.05) in total VEGF mRNA levels as well as VEGF₁₆₅ and VEGF splice variants containing exon 7 at 0, 2, and 6 h of recovery. The increase in VEGF mRNA was higher in the untrained than in the trained leg (P < 0.05). The results suggest that in human skeletal muscle, acute exercise increases total VEGF mRNA, an increase that appears to be explained mainly by an increase in VEGF₁₆₅ mRNA. Furthermore, 4 wk of training attenuated the exercise-induced response in skeletal muscle VEGF₁₆₅ mRNA.

angiogenesis; vascular endothelial growth factor₁₆₅; transient transcriptional activation

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