HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 288/1/R311    most recent 00399.2004v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (15) Citing Articles via Google Scholar Google Scholar Articles by Ji, L. L. Articles by Cunningham, J. T. Search for Related Content PubMed PubMed Citation Articles by Ji, L. L. Articles by Cunningham, J. T.

WATER AND ELECTROLYTE HOMEOSTASIS

Effects of water deprivation and rehydration on c-Fos and FosB staining in the rat supraoptic nucleus and lamina terminalis region

Departments of ¹Pharmacology and ²Physiology, and the Center for Biomedical Neuroscience, University of Texas Health Science Center at San Antonio, San Antonio, Texas

Submitted 15 June 2004 ; accepted in final form 23 September 2004

We studied cFos and FosB staining in the supraoptic nucleus (SON) the organum vasculosum of the lamina terminalis (OVLT) and the median preoptic nucleus (MnPO) in adult male rats after water deprivation (24 h, n = 11; 48 h, n = 12) and water deprivation with rehydration (22 h + water, n = 11; 46 h + water, n = 10). Control rats (n = 15) had water available ad libitum. Separate sets of serial sections from each brain were processed for immunocytochemistry using primary antibodies against either c-Fos or FosB protein. Plasma osmolality, vasopressin, hematocrit, and plasma proteins were measured in separate groups (n = 6–7). The number of c-Fos-positive cells in the SON was significantly increased after 24 and 48 h of water deprivation. In contrast, rehydrated groups were not different from control. Water deprivation significantly increased c-Fos staining in both the OVLT and the MnPO, but c-Fos staining was not altered by rehydration. FosB staining in the SON was significantly increased only by 48-h water deprivation, and this effect was significantly decreased by rehydration. In the MnPO and OVLT, FosB staining was significantly increased by water deprivation, and, like c-Fos staining, these increases were not affected by rehydration. Water deprivation significantly increased osmolality and hematocrit, as well as plasma protein and vasopressin concentrations. Plasma measurements from rehydrated rats were not different from control. We conclude that water deprivation and rehydration differentially affect c-Fos and FosB staining in a region-dependent manner.

vasopressin; thirst; anteroventral region of third ventricle; dehydration

This article has been cited by other articles:

				P. Shi, M. A. Martinez, A. S. Calderon, Q. Chen, J. T. Cunningham, and G. M. Toney Intra-carotid hyperosmotic stimulation increases Fos staining in forebrain organum vasculosum laminae terminalis neurones that project to the hypothalamic paraventricular nucleus J. Physiol., November 1, 2008; 586(21): 5231 - 5245. [Abstract] [Full Text] [PDF]

				C. Wotus, M. M. Arnhold, and W. C. Engeland Dehydration-induced drinking decreases Fos expression in hypothalamic paraventricular neurons expressing vasopressin but not corticotropin-releasing hormone Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2007; 292(3): R1349 - R1358. [Abstract] [Full Text] [PDF]

				J. Qiu, S. Yao, C. Hindmarch, V. Antunes, J. Paton, and D. Murphy Transcription Factor Expression in the Hypothalamo-Neurohypophyseal System of the Dehydrated Rat: Upregulation of Gonadotrophin Inducible Transcription Factor 1 mRNA Is Mediated by cAMP-Dependent Protein Kinase A J. Neurosci., February 28, 2007; 27(9): 2196 - 2203. [Abstract] [Full Text] [PDF]

				P. J. Mueller, M. J. Sullivan, R. R. Grindstaff, J. T. Cunningham, and E. M. Hasser Regulation of plasma vasopressin and renin activity in conscious hindlimb-unloaded rats Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2006; 291(1): R46 - R52. [Abstract] [Full Text] [PDF]

				H. B. Gottlieb, L. L. Ji, H. Jones, M. L. Penny, T. Fleming, and J. T. Cunningham Differential effects of water and saline intake on water deprivation-induced c-Fos staining in the rat Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2006; 290(5): R1251 - R1261. [Abstract] [Full Text] [PDF]

				L. Rinaman, R. R. Vollmer, J. Karam, D. Phillips, X. Li, and J. A. Amico Dehydration anorexia is attenuated in oxytocin-deficient mice Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2005; 288(6): R1791 - R1799. [Abstract] [Full Text] [PDF]