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Am J Physiol Regul Integr Comp Physiol 288: R1046-R1056, 2005. First published November 24, 2004; doi:10.1152/ajpregu.00286.2004
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WATER AND ELECTROLYTE HOMEOSTASIS

UT-B1 urea transporter is expressed along the urinary and gastrointestinal tracts of the mouse

N. Lucien,1 P. Bruneval,2 F. Lasbennes,3 M.-F. Belair,2 C. Mandet,2 J.-P. Cartron,1 P. Bailly,1 and M.-M. Trinh-Trang-Tan1

1INSERM U76, Institut National de la Transfusion Sanguine, Paris; 2INSERM U430, Hôpital Broussais, Paris; and 3CNRS, UMR 7519, Strasbourg, France

Submitted 30 April 2004 ; accepted in final form 17 November 2004

Selective transporters account for rapid urea transport across plasma membranes of several cell types. UT-B1 urea transporter is widely distributed in rat and human tissues. Because mice exhibit high urea turnover and are the preferred species for gene engineering, we have delineated UT-B1 tissue expression in murine tissues. A cDNA was cloned from BALB/c mouse kidney, encoding a polypeptide that differed from C57BL/6 mouse UT-B1 by one residue (Val-8-Ala). UT-B1 mRNA was detected by RT-PCR in brain, kidney, bladder, testis, lung, spleen, and digestive tract (liver, stomach, jejunum, colon). Northern blotting revealed seven UT-B1 transcripts in mouse tissues. Immunoblots identified a nonglycosylated UT-B1 protein of 29 kDa in most tissues and of 36 and 32 kDa in testis and liver, respectively. UT-B1 protein of gastrointestinal tract did not undergo N-glycosylation. Immunohistochemistry and in situ hybridization localized UT-B1 in urinary tract urothelium (papillary surface, ureter, bladder, and urethra), prominently on plasma membranes and restricted to the basolateral area in umbrella cells. UT-B1 was found in endothelial cells of descending vasa recta in kidney medulla and in astrocyte processes in brain. Dehydration induced by water deprivation for 2 days caused a tissue-specific decrease in UT-B1 abundance in the urinary bladder and the ureter.

cDNA; immunohistochemistry; urothelium; water deprivation


Address for reprint requests and other correspondence: M.-M. Trinh-Trang-Tan, INSERM U76, Institut National de Transfusion Sanguine, 6, rue Alexandre Cabanel, F-75015 Paris, France (E-mail: trinh{at}idf.inserm.fr)




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