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WATER AND ELECTROLYTE HOMEOSTASIS

Tyrosine kinase inhibition affects skate anion exchanger isoform I alterations after volume expansion

¹The Martin Boyer Laboratories, Department of Medicine, The University of Chicago, Chicago, Illinois; ³Department of Molecular Pharmacology, Physiology, and Biotechnology, Brown University, Providence, Rhode Island; and ²Mount Desert Island Biological Laboratory, Salsbury Cove, Maine

Submitted 6 October 2004 ; accepted in final form 4 November 2004

Upon exposure to hypotonic medium, skate red blood cells swell and then reduce their volume by releasing organic osmolytes and associated water. The regulatory volume decrease is inhibited by stilbenes and anion exchange inhibitors, suggesting involvement of the red blood cell anion exchanger skAE1. To determine the role of tyrosine phosphorylation, red blood cells were volume expanded with and without prior treatment with the tyrosine kinase inhibitor piceatannol. At the concentration used, 130 µM, piceatannol nearly completely inhibits p72^syk, a tyrosine kinase previously shown to phosphorylate skAE1 (M. W. Musch, E. H. Hubert, and L. Goldstein. J Biol Chem 274: 7923–7928, 1999). Hyposmotic-induced volume expansion stimulated association of p72^syk with a light membrane fraction of skate red blood cells. Piceatannol did not inhibit this association but decreased hyposmotically stimulated increased skAE1 tyrosine phophorylation. Movement of skAE1 from an intracellular to a surface detergent-resistant membrane domain and tetramer formation were not inhibited by piceatannol treatment. Two effects of hyposmotic-induced volume expansion, decreased band 4.1 binding and increased ankyrin, were both inhibited by piceatannol. These results suggest that at least one event requiring p72^syk activation is pivotal for hyposmotic-induced increased transport; however, steps that do not require tyrosine phosphorylation may also play a role.

band 4.1; ankyrin; detergent-resistant membranes; p72^syk

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