HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 289/4/R1155    most recent 00234.2005v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (8) Citing Articles via Google Scholar Google Scholar Articles by Studeny, S. Articles by Vizzard, M. A. Search for Related Content PubMed PubMed Citation Articles by Studeny, S. Articles by Vizzard, M. A.

WATER AND ELECTROLYTE HOMEOSTASIS

P2X2 and P2X3 receptor expression in postnatal and adult rat urinary bladder and lumbosacral spinal cord

¹Department of Neurology, and ²Department of Anatomy and Neurobiology, College of Medicine, University of Vermont, Burlington, Vermont

Submitted 5 April 2005 ; accepted in final form 6 June 2005

P2X receptors mediate the effects of ATP in micturition and nociception. During postnatal maturation, a spinobulbospinal reflex and voluntary voiding replace primitive voiding reflexes. This may involve changes in neuroactive compounds and receptors in bladder reflex pathways. We examined P2X2 and P2X3 receptors in bladder and spinal cord from postnatal (P0–P36, indicating number of days) and adult Wistar rats. Western blot of whole bladders for P2X2 and P2X3 expression was performed. Immunostaining for P2X2 and P2X3 receptors in urothelium and detrusor smooth muscle whole mounts and spinal cord sections was examined. Western blot demonstrated an age-dependent decrease (R² = 0.96, P 0.005) in P2X2 receptor expression in bladder, whereas P2X3 receptor expression in bladder peaked (P 0.005) during P14–P21. P2X2-immunoreactivity (IR) was present in urothelial cells, suburothelial plexus, detrusor smooth muscle, and serosa at birth, with staining in urothelial cells and serosa being most predominant. With increasing postnatal age, the intensity of P2X2-IR decreased in urothelial cells but increased in suburothelial plexus. P2X3-IR increased in urothelial cells and suburothelial plexus with postnatal age, whereas staining in detrusor and serosa remained relatively constant. At birth, P2X3-IR was present in the dorsal horn, lateral collateral pathway, and dorsal commissure. With increasing age, P2X3-IR was restricted to superficial dorsal horn and lateral collateral pathway. P2X2-IR was present in ependyme cells (S-100-IR) of the central canal as early as P2. These studies demonstrate plastic expression of P2X2 and P2X3 receptors in bladder and spinal cord during early postnatal development at times coincident with appearance of mature voiding patterns.

postnatal development; micturition reflexes; sacral parasympathetic nucleus; dorsal commissure; dorsal horn

This article has been cited by other articles:

				I. Milenkovic, I. Rinke, M. Witte, B. Dietz, and R. Rubsamen P2 Receptor-Mediated Signaling in Spherical Bushy Cells of the Mammalian Cochlear Nucleus J Neurophysiol, September 1, 2009; 102(3): 1821 - 1833. [Abstract] [Full Text] [PDF]