HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 289/4/R1177    most recent 00157.2005v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (3) Citing Articles via Google Scholar Google Scholar Articles by Vornanen, M. Articles by Krasnov, A. Search for Related Content PubMed PubMed Citation Articles by Vornanen, M. Articles by Krasnov, A.

COMPARATIVE AND EVOLUTIONARY PHYSIOLOGY

Steady-state effects of temperature acclimation on the transcriptome of the rainbow trout heart

¹University of Joensuu, Department of Biology, Joensuu, Finland; and ²University of Kuopio, Institute of Applied Biotechnology, Kuopio, Finland

Submitted 3 March 2005 ; accepted in final form 1 June 2005

Cold-acclimated (CA) phenotype of trout heart was induced by 4-wk acclimation at 4°C and was characterized by 32.7% increase in relative heart mass and 49.8% increase in ventricular myocyte size compared with warm-acclimated (WA; 18°C) fish (P < 0.001). Effect of temperature acclimation on transcriptome of the rainbow trout heart was examined using species-specific microarray chips containing 1,380 genes. After 4 wk of temperature acclimation, 8.8% (122) of the genes were differently expressed in CA and WA hearts, and most of them (82%) were upregulated in the cold (P < 0.01). Transcripts of genes engaged in protein synthesis and intermediary metabolism were most strongly upregulated, whereas genes contributing to the connective tissue matrix were clearly repressed. Extensive upregulation of the genes coding for ribosomal proteins and translation elongation and initiation factors suggest that the protein synthesis machinery of the trout heart is enhanced in the cold and is an essential part of the compensatory mechanism causing and maintaining the hypertrophy of cardiac myocytes. The prominent depression of collagen genes may be indicative of a reduced contribution of extracellular matrix to the remodeling of the CA fish heart. Temperature-related changes in transcripts of metabolic enzymes suggest that at mRNA level, glycolytic energy production from carbohydrates is compensated in the heart of CA rainbow trout, while metabolic compensation is absent in mitochondria. In addition, the analysis revealed three candidate genes: muscle LIM protein, atrial natriuretic peptide B, and myosin light chain 2, which might be central for induction and maintenance of the hypertrophic phenotype of the CA trout heart. These findings indicate that extensive modification of gene expression is needed to maintain the temperature-specific phenotype of the fish heart.

gene expression; fish heart; temperature-induced hypertrophy

This article has been cited by other articles:

				M. Hassinen, V. Paajanen, J. Haverinen, H. Eronen, and M. Vornanen Cloning and expression of cardiac Kir2.1 and Kir2.2 channels in thermally acclimated rainbow trout Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2007; 292(6): R2328 - R2339. [Abstract] [Full Text] [PDF]

				B. A. Buckley Comparative environmental genomics in non-model species: using heterologous hybridization to DNA-based microarrays J. Exp. Biol., May 1, 2007; 210(9): 1602 - 1606. [Abstract] [Full Text] [PDF]

				B. A. Buckley, A. Y. Gracey, and G. N. Somero The cellular response to heat stress in the goby Gillichthys mirabilis: a cDNA microarray and protein-level analysis J. Exp. Biol., July 15, 2006; 209(14): 2660 - 2677. [Abstract] [Full Text] [PDF]