HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplemental Tables and Figures All Versions of this Article: 290/2/R467    most recent 00507.2005v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (7) Citing Articles via Google Scholar Google Scholar Articles by Nag, K. Articles by Hirose, S. Search for Related Content PubMed PubMed Citation Articles by Nag, K. Articles by Hirose, S.

COMPARATIVE AND EVOLUTIONARY PHYSIOLOGY

Molecular and functional characterization of adrenomedullin receptors in pufferfish

¹Department of Biological Sciences, Tokyo Institute of Technology, Yokohama, Japan; and ²Ocean Research Institute, The University of Tokyo, Tokyo, Japan

Submitted 12 July 2005 ; accepted in final form 20 September 2005

The receptors for the calcitonin gene-related peptide (CGRP)/adrenomedullin (AM) family peptides were characterized in the mefugu Takifugu obscurus, a euryhaline fugu species very close to Takifugu rubripes, which has as many as five adrenomedullin genes (AM1–5). CGRP and AM share a G protein-coupled core receptor called calcitonin receptor-like receptor (CLR), and the specificity of the CLR is determined by the interaction with receptor activity-modifying proteins (RAMPs). Through database mining, three CLRs (CLR1–3) and five RAMPs (RAMP1–5) were identified, and all of them were cloned by RT-PCR and characterized by functional expression in COS7 cells in every possible combination of CLR-RAMP. The following combinations generated cAMP in response to physiological concentrations of CGRP, AM1 (an ortholog of mammalian AM), AM2, and AM5: CLR1-RAMP1/4 (CGRP), CLR1-RAMP2/3/5 (AM1), CLR2-RAMP2 (AM1), CLR1-RAMP3 (AM2), and CLR1-RAMP3 (AM5). Their expressions were found by Northern blot analysis to be tissue specific and salinity dependent. For example, CLR1-RAMP5 and CLR1-RAMP2 are expressed specifically in the gill and kidney, respectively, suggesting their involvement in osmoregulation. Furthermore, relatively high levels of CLRs and RAMPs were found in the spleen and ovary, suggesting roles in the immune and female reproductive systems. Immunohistochemistry revealed that AM receptors of the following types are expressed in the locations, indicated in brackets, of the mefugu gill and kidney: CLR1-RAMP5 (interlamellar vessels), CLR2-RAMP2 (pillar cells), and CLR1-RAMP2 (apical side of renal proximal tubule cells).

calcitonin receptor-like receptor; pillar cell; proximal tubule; receptor activity-modifying proteins

This article has been cited by other articles:

				M. Ogoshi, S. Nobata, and Y. Takei Potent osmoregulatory actions of homologous adrenomedullins administered peripherally and centrally in eels Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2008; 295(6): R2075 - R2083. [Abstract] [Full Text] [PDF]

				S. Nobata, M. Ogoshi, and Y. Takei Potent cardiovascular actions of homologous adrenomedullins in eels Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2008; 294(5): R1544 - R1553. [Abstract] [Full Text] [PDF]

				A. Kato, K. Nakamura, H. Kudo, Y. H. Tran, Y. Yamamoto, H. Doi, and S. Hirose Characterization of the Column and Autocellular Junctions That Define the Vasculature of Gill Lamellae J. Histochem. Cytochem., September 1, 2007; 55(9): 941 - 953. [Abstract] [Full Text] [PDF]