HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 291/4/R889    most recent 00536.2005v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (10) Citing Articles via Google Scholar Google Scholar Articles by Teske, J. A. Articles by Kotz, C. M. Search for Related Content PubMed PubMed Citation Articles by Teske, J. A. Articles by Kotz, C. M.

APPETITE, OBESITY, DIGESTION, AND METABOLISM

Elevated hypothalamic orexin signaling, sensitivity to orexin A, and spontaneous physical activity in obesity-resistant rats

¹Department of Food Science and Nutrition, University of Minnesota, Saint Paul; ²Departments of Medicine, Psychology, and Psychiatry, University of Minnesota, Minneapolis; ³Minnesota Obesity Center; ⁴Veterans Affairs Medical Center, Minneapolis; and ⁵Mayo Clinic Rochester, Endocrine Research Unit, Rochester, Minnesota

Submitted 21 July 2005 ; accepted in final form 23 May 2006

Selectively-bred obesity-resistant [diet resistant (DR)] rats weigh less than obesity-prone [diet-induced obese (DIO)] rats, despite comparable daily caloric intake, suggesting phenotypic energy expenditure differences. Human data suggest that obesity is maintained by reduced ambulatory or spontaneous physical activity (SPA). The neuropeptide orexin A robustly stimulates SPA. We hypothesized that DR rats have greater: 1) basal SPA, 2) orexin A-induced SPA, and 3) preproorexin, orexin 1 and 2 receptor (OX1R and OX2R) mRNA, compared with DIO rats. A group of age-matched out-bred Sprague-Dawley rats were used as additional controls for the behavioral studies. DIO, DR, and Sprague-Dawley rats with dorsal-rostral lateral hypothalamic (rLHa) cannulas were injected with orexin A (0, 31.25, 62.5, 125, 250, and 500 pmol/0.5 µl). SPA and food intake were measured for 2 h after injection. Preproorexin, OX1R and OX2R mRNA in the rLHa, and whole hypothalamus were measured by real-time RT-PCR. Orexin A significantly stimulated feeding in all rats. Orexin A-induced SPA was significantly greater in DR and Sprague-Dawley rats than in DIO rats. Two-mo-old DR rats had significantly greater rLHa OX1R and OX2R mRNA than DIO rats but comparable preproorexin levels. Eight-mo-old DR rats had elevated OX1R and OX2R mRNA compared with DIO rats, although this increase was significant for OX2R only at this age. Thus DR rats show elevated basal and orexin A-induced SPA associated with increased OX1R and OX2R gene expression, suggesting that differences in orexin A signaling through OX1R and OX2R may mediate DIO and DR phenotypes.

hypocretin; lateral hypothalamus; locomotor activity; diet-induced obesity

This article has been cited by other articles:

				J. A. Teske and C. M. Kotz Effect of acute and chronic caloric restriction and metabolic glucoprivation on spontaneous physical activity in obesity-prone and obesity-resistant rats Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2009; 297(1): R176 - R184. [Abstract] [Full Text] [PDF]

				C. M. Kotz, J. A. Teske, and C. J. Billington Neuroregulation of nonexercise activity thermogenesis and obesity resistance Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2008; 294(3): R699 - R710. [Abstract] [Full Text] [PDF]

				B. E. Levin Orexins: neuropeptides for all seasons and functions Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2006; 291(4): R885 - R888. [Full Text] [PDF]