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DEVELOPMENTAL PHYSIOLOGY AND PREGNANCY

Differential regulation of MMPs and matrix assembly in chicken and turkey growth-plate chondrocytes

¹Department of Biochemistry and Nutrition, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University, Israel; and ²Institute of Animal Science, the Volcani Center, Bet Dagan, Israel

Submitted 12 December 2006 ; accepted in final form 27 February 2007

Matrix metalloproteinases (MMPs) play a crucial role in growth-plate vascularization and ossification by processes involving proteolytic cleavage and remodeling of the extracellular matrix (ECM). Their regulation in the growth plate is crucial for normal vs. impaired matrix assembly. Tibial dyschondroplasia (TD), a prevalent skeletal abnormality in avian species, is characterized by the formation of a nonvascularized, nonmineralized plaque in the growth plate. Here, we show differential regulation of MMPs in cultured chondrocytes from chickens and turkeys; retinoic acid (RA) elevated MMP-2 activity in both species, but only in chicken did it induce MMP-9 activity. In contrast, phorbol 12-myristate 13-acetate (PMA) treatment induced MMP-9 activity in turkey chondrocytes but not in those of chicken. Moreover, we found different developmental patterns of TD in chickens and turkeys in-vivo as lower concentrations of, and shorter exposure to thiram were required in chicken than in turkey for TD induction. Growth-plate cartilage taken from thiram-induced lesions had lower gelatinolytic and caseinolytic activities compared with normal cartilage. Likewise, thiram reduced MMP-2 and MMP-13 activity in both chicken and turkey chondrocytes in vitro, although 10-fold higher concentrations were required for this effect in the latter. Finally, the combined treatments of RA or PMA with thiram induced MMP-9 activity in turkey but not in chicken chondrocytes. Furthermore, RA combined with thiram synergistically upregulated its activity in turkey but not chicken chondrocytes. Taken together, these results suggest that mechanisms of MMP regulation differ in the growth plates of these closely related avian species, resulting in altered matrix assembly as exemplified by TD development.

gelatinase; collagenase; retinoic acid; phorbol 12-myristate 13-acetate; thiram; tibial dyschondroplasia

This article has been cited by other articles:

				M. Hasky-Negev, S. Simsa, A. Tong, O. Genina, and E. Monsonego Ornan Expression of matrix metalloproteinases during vascularization and ossification of normal and impaired avian growth plate J Anim Sci, June 1, 2008; 86(6): 1306 - 1315. [Abstract] [Full Text] [PDF]

				S. Simsa, A. Hasdai, H. Dan, and E. M. Ornan Induction of Tibial Dyschondroplasia in Turkeys by Tetramethylthiuram Disulfide (Thiram) Poult. Sci., August 1, 2007; 86(8): 1766 - 1771. [Abstract] [Full Text] [PDF]