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Am J Physiol Regul Integr Comp Physiol 293: R1608-R1618, 2007. First published July 18, 2007; doi:10.1152/ajpregu.00207.2006
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NEUROHUMORAL CONTROL OF CARDIOVASCULAR FUNCTION

Hydrogen sulfide downregulates the aortic L-arginine/nitric oxide pathway in rats

Bin Geng,1,2 Yuying Cui,1 Jing Zhao,1,2 Fang Yu,2 Yi Zhu,2 Geyang Xu,2 Zhiwen Zhang,2 Chaoshu Tang,1,2 and Junbao Du3

1Institute of Cardiovascular Research, First Hospital of Peking University; 2Department of Physiology, Peking University Health Science Center; and 3Department of Pediatric, First Hospital of Peking University, Beijing, People's Republic of China

Submitted 22 March 2006 ; accepted in final form 27 June 2007

The aim of the present study was to investigate the effect of hydrogen sulfide (H2S) signaling by nitric oxide (NO) in isolated rat aortas and cultured human umbilical vein endothelial cells (HUVECs). Both administration of H2S and NaHS, as well as endogenous H2S, reduced NO formation, endothelial nitric oxide synthase (eNOS) activity, eNOS transcript abundance, and L-arginine (L-Arg) transport (all P < 0.01). The kinetics analysis of eNOS activity and L-Arg transport showed that H2S reduced Vmax values (all P < 0.01) without modifying Km parameters. Use of selective NOS inhibitors verified that eNOS [vs. inducible NOS (iNOS) and neuronal NOS (nNOS)] was the specific target of H2S regulation. H2S treatment (100 µmol/l) reduced Akt phosphorylation and decreased eNOS phosphorylation at Ser1177. H2S reduced L-Arg uptake by inhibition of a system y+ transporter and decreased the CAT-1 transcript. H2S treatment reduced protein expression of eNOS but not of nNOS and iNOS. Pinacidil (KATP channel opener) exhibited the similar inhibitory effects on the L-Arg/NOS/NO pathway. Glibenclamide (KATP channel inhibitor) partly blocked the inhibitory effect of H2S and pinacidil. An in vivo experiment revealed that H2S downregulated the vascular L-Arg/eNOS/NO pathway after intraperitoneal injection of NaHS (14 µmol/kg) in rats. Taken together, our findings suggest that H2S downregulates the vascular L-Arg/NOS/NO pathway in vitro and in vivo, and the KATP channel could be involved in the regulatory mechanism of H2S.

nitric oxide; nitric oxide synthase; L-arginine transport


Address for reprint requests and other correspondence: B. Geng, Institute of Cardiovascular Research, First Hospital of Peking Univ., Beijing xishuku St. 8, 100034 (e-mail: bingeng{at}bjmu.edu.cn)






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