HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 294/1/R192    most recent 00669.2007v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Trappe, T. A. Articles by Magnusson, S. P. Search for Related Content PubMed PubMed Citation Articles by Trappe, T. A. Articles by Magnusson, S. P.

ENVIRONMENTAL, EXERCISE AND RESPIRATORY PHYSIOLOGY

Cyclooxygenase mRNA expression in human patellar tendon at rest and after exercise

Human Performance Laboratory, Ball State University, Muncie, Indiana; and Institute for Sports Medicine Copenhagen, Bispebjerg Hospital, Copenhagen, Denmark

Submitted 14 September 2007 ; accepted in final form 30 October 2007

Exercise has been shown to acutely elevate several metabolic processes in tendon tissue, including collagen turnover and blood flow, and chronically induce changes in tendon properties. Many of these acute metabolic responses to exercise are regulated by the cyclooxygenase (COX) enzymes. We measured the expression levels of COX-1 [variants 1 and 2 (COX-1v1 and COX-1v2)], COX-2, and the recently discovered intron 1-retaining COX-1 variants (COX-1b₁, COX-1b₂, and COX-1b₃) at rest and after resistance exercise (RE). Patellar tendon biopsy samples were taken from six individuals (3 men and 3 women) before and 4 h after a bout of RE (3 sets of 10 repetitions at 70% of 1 repetition maximum) and from a separate group of six individuals (3 men and 3 women) before and 24 h after RE and analyzed by real-time RT-PCR. The COX-1 variants were the most abundant COX mRNAs before exercise and remained unchanged (P > 0.05) after exercise. COX-2 was also expressed in tendon tissue at rest and was unchanged (P > 0.05) after exercise. The intron 1-retaining COX-1 variants were not detectable in tendon tissue before or after exercise. COX-1 and COX-2 were expressed at much higher levels by the patellar tendon than by quadriceps skeletal muscle, although the overall COX mRNA expression patterns were similar in skeletal muscle and tendon (COX-1v2 > COX-1v1, P < 0.05; ratio of COX-1 to COX-2 4:1). These results suggest that COX-1 and COX-2 are constitutively expressed at relatively high levels in human patellar tendon and are likely targets of COX-inhibiting drugs at rest and after physical activity.

real-time reverse transcription-polymerase chain reaction; cyclooxygenase-1; cyclooxygenase-2; cycooxygenase-3; cyclooxygenase-1b