HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 295/3/R806    most recent 90540.2008v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (5) Citing Articles via Google Scholar Google Scholar Articles by Pandke, K. E. Articles by Dyck, D. J. Search for Related Content PubMed PubMed Citation Articles by Pandke, K. E. Articles by Dyck, D. J.

ENDOCRINE PHYSIOLOGY AND METABOLISM

Decreasing intramuscular phosphagen content simultaneously increases plasma membrane FAT/CD36 and GLUT4 transporter abundance

Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada

Submitted 27 June 2008 ; accepted in final form 15 July 2008

Decreasing muscle phosphagen content through dietary administration of the creatine analog β-guanidinopropionic acid (β-GPA) improves skeletal muscle oxidative capacity and resistance to fatigue during aerobic exercise in rodents, similar to that observed with endurance training. Surprisingly, the effect of β-GPA on muscle substrate metabolism has been relatively unexamined, with only a few reports of increased muscle GLUT4 content and insulin-stimulated glucose uptake/clearance in rodent muscle. The effect of chronically decreasing muscle phophagen content on muscle fatty acid (FA) metabolism (transport, oxidation, esterification) is virtually unknown. The purpose of the present study was to examine changes in muscle substrate metabolism in response to 8 wk feeding of β-GPA. Consistent with other reports, β-GPA feeding decreased muscle ATP and total creatine content by 50 and 90%, respectively. This decline in energy charge was associated with simultaneous increases in both glucose (GLUT4; +33 to 45%, P < 0.01) and FA (FAT/CD36; +28 to 33%, P < 0.05) transporters in the sarcolemma of red and white muscle. Accordingly, we also observed significant increases in insulin-stimulated glucose transport (+47%, P < 0.05) and AICAR-stimulated palmitate oxidation (+77%, P < 0.01) in the soleus muscle of β-GPA-fed animals. Phosphorylation of AMPK (+20%, P < 0.05), but not total protein, was significantly increased in both fiber types in response to muscle phosphagen reduction. Thus the content of sarcolemmal transporters for both of the major energy substrates for muscle increased in response to a reduced energy charge. Increased phosphorylation of AMPK may be one of the triggers for this response.

β-guanidinopropionic acid; rat; skeletal muscle; energy charge; substrate metabolism

This article has been cited by other articles:

				D. B. Williams, L. N. Sutherland, M. R. Bomhof, S. A. U. Basaraba, A. B. Thrush, D. J. Dyck, C. J. Field, and D. C. Wright Muscle-specific differences in the response of mitochondrial proteins to {beta}-GPA feeding: an evaluation of potential mechanisms Am J Physiol Endocrinol Metab, June 1, 2009; 296(6): E1400 - E1408. [Abstract] [Full Text] [PDF]

				K. L. Mullen, J. Pritchard, I. Ritchie, L. A. Snook, A. Chabowski, A. Bonen, D. Wright, and D. J. Dyck Adiponectin resistance precedes the accumulation of skeletal muscle lipids and insulin resistance in high-fat-fed rats Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2009; 296(2): R243 - R251. [Abstract] [Full Text] [PDF]