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EXERCISE AND RESPIRATORY PHYSIOLOGY

Metabolic, enzymatic, and transporter responses in human muscle during three consecutive days of exercise and recovery

Department of Kinesiology, University of Waterloo, Waterloo, Ontario, Canada

Submitted 6 March 2008 ; accepted in final form 18 July 2008

This study investigated the responses in substrate- and energy-based properties to repetitive days of prolonged submaximal exercise and recovery. Twelve untrained volunteers (O_2peak = 44.8 ± 2.0 ml·kg^–1·min^–1, mean ± SE) cycled (60 O_2peak) on three consecutive days followed by 3 days of recovery. Tissue samples were extracted from the vastus lateralis both pre- and postexercise on day 1 (E1), day 3 (E3), and during recovery (R1, R2, R3) and were analyzed for changes in metabolism, substrate, and enzymatic and transporter responses. For the metabolic properties (mmol/kg^–1 dry wt), exercise on E1 resulted in reductions (P < 0.05) in phosphocreatine (PCr; 80 ± 1.9 vs. 41.2 ± 3.0) and increases (P < 0.05) in inosine monophosphate (IMP; 0.13 ± 0.01 vs. 0.61 ± 0.2) and lactate (3.1 ± 0.4 vs. 19.2 ± 4.3). At E3, both IMP and lactate were lower (P < 0.05) during exercise. For the transporters, the experimental protocol resulted in a decrease (P < 0.05) in glucose transporter-1 (GLUT1; 29% by R1), an increase in GLUT4 (29% by E3), and increases (P < 0.05) for both monocarboxylate transporters (MCT) (for MCT1, 23% by R2 and for MCT4, 18% by R1). Of the mitochondrial and cytosolic enzyme activities examined, cytochrome c oxidase (COX), and hexokinase were both reduced (P < 0.05) by exercise at E1 and in the case of hexokinase and phosphorylase by exercise on E3. With the exception at COX, which was lower (P < 0.05) at R1, no differences in enzyme activities existed at rest between E, E3, and recovery days. Results suggest that the glucose and lactate transporters are among the earliest adaptive responses of substrate and metabolic properties studied to the sudden onset of regular low-intensity exercise.

contractile activity; glucose; lactate; transporters; vastus lateralis; glycogen; energy metabolism; enzyme activity

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