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Am J Physiol Regul Integr Comp Physiol 281: R359-R364, 2001;
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Vol. 281, Issue 1, R359-R364, July 2001

RAPID COMMUNICATION
Heat shock prevents simulated ischemia-induced apoptosis in renal tubular cells via a PKC-dependent mechanism

K. K. Meldrum, D. R. Meldrum, S. F. Sezen, J. K. Crone, and A. L. Burnett

Departments of Urology and Surgery, Johns Hopkins University, Baltimore, Maryland 21287


    ABSTRACT
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

Heat shock produces cellular tolerance to a variety of adverse conditions; however, the protective effect of heat shock on renal cell ischemic injury remains unclear. Protein kinase C (PKC) has been implicated in the signaling mechanisms of acute preconditioning, yet it remains unknown whether PKC mediates heat shock-induced delayed preconditioning in renal cells. To study this, renal tubular cells (LLC-PK1) were exposed to thermal stress (43°C) for 1 h and heat shock protein (HSP) 72 induction was confirmed by Western blot analysis. Cells were subjected to simulated ischemia 24 h after thermal stress, and the effect of heat shock (delayed preconditioning) on ischemia-induced apoptosis (terminal deoxynucleotidyl transferase dUTP nick-end labeling) and B cell lymphoma 2 (Bcl2) expression (Western) was determined. Subsequently, the effect of PKC inhibition on HSP72 induction and heat stress-induced ischemic tolerance was evaluated. Thermal stress induced HSP72 production, increased Bcl2 expression, and prevented simulated ischemia-induced renal tubular cell apoptosis. PKC inhibition abolished thermal induction of HSP72 and prevented heat stress-induced ischemic tolerance. These data demonstrate that thermal stress protects renal tubular cells from simulated ischemia-induced apoptosis through a PKC-dependent mechanism.

kidney; preconditioning; hypoxia; B cell lymphoma 2


    INTRODUCTION
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

HEAT SHOCK PRODUCES cellular tolerance against a variety of adverse conditions. This phenomenon is thought to be due, in part, to the production of heat shock proteins (HSPs). HSPs are rapidly synthesized in response to cellular stress and function as molecular chaperones, regulating the synthesis, translocation, and degradation of proteins (2, 7, 13, 43). The HSP70 family includes the constitutively expressed HSP73, present in normal cells, and the highly stress-inducible HSP72 (43). In the kidney, HSP72 production localizes primarily to renal tubular cells (40). Induction of HSP72 occurs 24 h after heat stress, ischemia, and exposure to cellular toxins (3, 8, 29, 39, 41, 45), and its expression confers cellular resistance to further heat stress and toxin exposure (37, 45). Although HSP72 expression has also been shown to protect cells from ischemic injury (25, 44), its cytoprotective role in renal ischemia remains controversial. The ability of heat shock and HSP72 to protect renal cells from ischemia has been inconsistent in both animal models of renal ischemia-reperfusion (I/R) injury (15, 31) and in vitro models of renal tubular cell hypoxia-ischemia (17, 37, 41, 42).

Renal tubular epithelial cells have been demonstrated to be the cell type most susceptible to renal ischemic injury (6, 11, 22, 34). Ischemic intervals <1 h primarily result in renal tubular cell death through apoptosis (22, 34), a process by which cells use their own energy sources and proteins to undergo nonnecrotic "suicide" (16, 35, 36). As apoptosis is a gene-driven process, it is uniquely suited to molecular modulation and possible inhibition. Recently, investigators demonstrated that HSP72 inhibits apoptosis through a variety of mechanisms (4, 9, 10, 14, 21, 30), and Wang and colleagues (41) further demonstrated that heat stress inhibits apoptosis in ATP-depleted renal tubular cells (41).

The mechanisms of HSP72 induction remain unclear. Lee et al. (20) showed that protein kinase C (PKC) inhibition prevents heat stress-induced thermotolerance in human colon carcinoma cells, and Yamashita and colleagues (44) established that PKC inhibition suppresses the cardioprotective effect of heat stress in animals. PKC is a known mediator of acute ischemic preconditioning in myocardium (1, 5, 26-28, 32) and, indeed, may prove to be an important mediator of HSP72-induced delayed preconditioning and cytoprotection. With the use of an in vitro model of renal tubular cell ischemia in LLC-PK1 cells, the purposes of this study were, therefore, to determine whether 1) heat shock induces renal tubular HSP72, 2) HSP72 induction prevents ischemia-induced apoptosis, and 3) PKC mediates heat stress-induced ischemic tolerance.


    MATERIALS AND METHODS
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

Cell culture. The renal tubular epithelial cell line LLC-PK1 established from pig renal cortex was cultured in MEDIUM 199 EBSS (LTI, Gaithersburg, MD) supplemented with 10% fetal bovine serum (FBS). The cells were passaged weekly by trypsinization (0.25% trypsin, 0.02% EDTA) after formation of a confluent monolayer and were placed in serum-free media (0.25% FBS) 24 h before stimulation.

Simulated ischemia. After formation of a confluent epithelial cell sheet, the cells were washed twice with PBS and the monolayer was immersed in mineral oil, simulating ischemic conditions by restricting cell nutrients and oxygen and limiting metabolite washout (12, 38). The cells were exposed to 1 h of simulated ischemia followed by 24 h of substrate replacement (reimmersion in MEDIUM 199 + 0.25% FBS).

Heat shock. After formation of a confluent epithelial sheet, the cells were immersed in a 43°C water bath for 1 h, with or without 2 µM chelerythrine chloride or 2 µM bisindolylmaleimide [PKC inhibitors, Sigma, St. Louis, MO; doses previously shown to be effective in renal tubular cells (24, 33)] pretreatment. Twenty-four hours after thermal stress, the cells were either collected for Western blot analysis or exposed to 1 h of simulated ischemia and 24 h of substrate replacement with subsequent Western blot analysis or apoptosis quantification.

Western blotting. Western blot analysis was performed on control samples, samples exposed to thermal stress in the presence or absence of PKC inhibition, samples exposed to simulated ischemia in the presence or absence of thermal preconditioning, and samples exposed to simulated ischemia in the presence of both thermal preconditioning and PKC inhibition. Each 100-mm confluent plate of cells was washed twice with PBS, scraped, and centrifuged. The supernatant was removed, and the cell pellet was lysed using a 2% SDS solution in protease inhibitor buffer (6.05 g Tris, 0.38 g EDTA, and 0.38 g EGTA in 1.0 l, pH 7.4) supplemented with aprotinin (5 µg/ml), leupeptin (1 µg/ml), and pefabloc (1 mM). After filtration through a QIAshredder (Qiagen, Valencia, CA), the protein extracts (200 µg/lane) were electrophoresed on a 10% SDS-polyacrylamide gel and transferred to a nitrocellulose membrane. Immunoblotting was performed by incubating each membrane in 5% dry milk for 1 h, followed by incubation with either an anti-HSP72 rabbit polyclonal antibody (1:1,000, StressGen, British Columbia, Canada) or an anti-B cell lymphoma 2 (Bcl2) mouse monoclonal antibody (1:1,000, LabVision, Fremont, CA) for 2 h. After washing twice in Tris-PBS, each membrane was incubated for 2 h with a peroxidase-conjugated secondary antibody (1:10,000, StressGen). The membranes were then developed using enhanced chemiluminescence (Amersham Pharmacia Biotech, Piscataway, NJ).

Quantitation of apoptosis. Cells were plated in four-well chamber slides at 1.5 × 104 cells per well and grown in 1 ml of MEDIUM 199 supplemented with 10% FBS until a confluent monolayer was obtained. The cells were then washed twice in PBS, and the monolayer was immersed in 1 ml of mineral oil. The cells were exposed to either 1 ml of media alone (control), 1 ml of media supplemented with 100 µl of mineral oil (control), 1 ml of media supplemented with either 2 µM chelerythrine chloride or 2 µM bisindolylmaleimide (controls), 1 h of simulated ischemia followed by 24 h of substrate replacement, or 1 h of heat stress (with or without 2 µM chelerythrine chloride or 2 µM bisindolylmaleimide pretreatment) followed by 1 h of simulated ischemia and 24 h of substrate replacement (reimmersion in MEDIUM 199 + 0.25% FBS). Four treatment chambers in each experimental group were quantified for apoptosis using a kit from Boehringer Mannheim (Indianapolis, IN). The kit is based on terminal deoxynucleotidyl transferase incorporation of fluorescein-dUTP to detect DNA strand breaks in the nuclei of cells undergoing apoptosis. The cell nuclei in each well were then counterstained with bis-benzimide (10 µg/ml in PBS) for 30 s. The number of apoptotic nuclei was counted in three nonoverlapping ×320 microscope fields/well and averaged. The entire protocol was then repeated in triplicate.

Cell viability. Cells were seeded at 5.0 × 105 and grown in 3 ml of media in 60-mm petri dishes until a confluent monolayer was obtained. With the use of the treatment groups described above, a cell suspension in PBS was prepared from each culture dish. Trypan blue (0.4%) was added to each cell suspension, and the mixture was allowed to stand at room temperature for 5 min. The number of viable cells was counted in each sample and expressed as a percentage of the total cell count.

Statistical analysis. Data are presented as mean values ± SE. Differences at the 95% confidence level were considered significant. The experimental groups were compared using ANOVA with post hoc Bonferroni-Dunn (StatView 4.5, Berkeley, CA).


    RESULTS
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

Induction of HSP72 after heat stress. HSP72 induction by thermal stress was verified by Western blot analysis. Control samples expressed low levels of HSP72, whereas 1 h of thermal stress induced a marked increase in HSP72 expression (Fig. 1).


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  		Fig. 1.   Western blot demonstrating thermal induction of heat shock protein (HSP) 72 (43°C; C, control) and the prevention of thermal HSP72 induction with protein kinase C (PKC) inhibition [2 µM chelerythrine (Chel) + 43°C and 2 µM bisindolylmaleimide (Bis; +43°C)].

Evaluation and quantitation of apoptosis after simulated ischemia. Cells exposed to media alone, media supplemented with 100 µl of mineral oil, or media supplemented with either 2 µM chelerythrine chloride or 2 µM bisindolylmaleimide did not undergo apoptosis [0.667 ± 0.333, 1.33 ± 0.882, 0.777 ± 0.619, and 2.7 ± 1.2 apoptotic nuclei/high power field (hpf), respectively]. In contrast, 1 h of simulated ischemia and 24 h of substrate replacement induced a significant degree of apoptosis (85 ± 14 apoptotic nuclei/hpf, P < 0.05 vs. control, Figs. 2B and 3). Cells exposed to media alone, media supplemented with 100 µl of mineral oil, and media supplemented with either 2 µM chelerythrine chloride or 2 µM bisindolylmaleimide demonstrated 90 ± 2.1, 84 ± 2.8, 83 ± 2.0, and 87 ± 1.5% viability, respectively, whereas cells exposed to simulated ischemia demonstrated 81 ± 5.4% viability (not significantly different from controls).


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  		Fig. 2.   Photographs (magnification ×320) demonstrating renal tubular cell apoptosis (terminal deoxynucleotidyl transferase dUTP nick-end labeling assay) and the effects of heat preconditioning and PKC inhibition on 60 min of simulated ischemia. A, control cells; B, cells exposed to 60 min of simulated ischemia; C, cells exposed to simulated ischemia 24 h after thermal stress; D, cells exposed to simulated ischemia 24 h after PKC inhibition and thermal stress. Sixty minutes of ischemia induced apoptosis in the majority of cells. Heat preconditioning prevented ischemia-induced apoptosis, whereas the presence of chelerythrine chloride blocked the protective effect of heat preconditioning and resulted in a significant degree of apoptosis.



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  		Fig. 3.   Graph depicting the number of apoptotic nuclei per high powered field (hpf) (×320) in each treatment group examined. Thermal preconditioning prevented ischemia-induced apoptosis. This effect was blocked by administering a PKC inhibitor before thermal stress (2 µM Chel; 2 µM Bis).

Effect of thermal stress on simulated ischemia-induced apoptosis. Heat preconditioning of renal tubular cells prevented simulated ischemia-induced apoptosis as shown in Figs. 2C and 3 (14 ± 9 apoptotic nuclei/hpf). Cell viability in this treatment group was not significantly different from controls at 78 ± 4%.

Effect of PKC on HSP72 expression and heat stress-induced ischemic tolerance. PKC inhibition with either 2 µM chelerythrine chloride or 2 µM bisindolylmaleimide abolished thermal induction of HSP72 (Fig. 1) and prevented heat stress-induced ischemic tolerance. Cells treated with either 2 µM chelerythrine chloride or 2 µM bisindolylmaleimide before heat stress demonstrated a significant degree of apoptosis in response to simulated ischemia (78 ± 9 and 64 ± 9 apoptotic nuclei/hpf, respectively, P < 0.05 vs. control, Figs. 2D and 3), whereas cell viability was not significantly different from controls (81 ± 5.8 and 77 ± 7.2%).

Effect of simulated ischemia, thermal preconditioning, and PKC inhibition on Bcl2 expression. Simulated ischemia reduced Bcl2 expression compared with control samples (Fig. 4). Although Bcl2 expression approached control levels on cell exposure to heat stress and simulated ischemia, PKC inhibition with either 2 µM chelerythrine chloride or 2 µM bisindolylmaleimide abolished this response.


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  		Fig. 4.   Western blot demonstrating expression of B cell lymphoma 2 (Bcl2). Control samples (C), samples exposed to 2 µM Bis, and samples exposed to 2 µM Chel demonstrated significant expression of Bcl2. After simulated ischemia (I), Bcl2 expression decreased; however, this response was ameliorated by prior heat preconditioning (43°C+I). Heat preconditioning in the presence of PKC inhibition (43°C+I+Chel; 43°C+I+Bis) decreased Bcl2 expression.


    DISCUSSION
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

Thermal preconditioning confers cellular resistance against a variety of adverse conditions, in part, through the production of HSPs. HSP72, a highly stress-inducible HSP, is known to protect a variety of cells from thermal injury, toxins, and ischemia (37, 44, 45). In renal tubular cells, HSP72 is protective against thermal injury and toxins; however, its cytoprotective role in ischemia has been unclear in both animal and in vitro models (15, 17, 31, 37, 41, 42). Although overexpression of HSP72 (via transfection) in LLC-PK1 cells has been demonstrated to protect against oxidative cellular injury (H2O2 exposure) (17), and heat stress induction of HSP72 (43°C × 1 h) has been demonstrated to protect against ATP depletion-induced apoptosis (opossum kidney tubular cells) (41, 42), transfected HSP72 has not been shown to protect against hypoxic injury in LLC-PK1 cells, as measured by loss of intracellular luciferase activity (37). To clarify the cytoprotective role of heat stress in renal tubular cell ischemia, we investigated the effect of heat stress induction of HSP72 on ischemia-induced apoptosis in LLC-PK1 cells using an oil immersion model of simulated ischemia. This model uniquely simulates in vivo renal cell I/R injury by restricting cell exposure to oxygen and nutrients and preventing metabolite washout. In addition, as the mechanisms of renal HSP72 induction remain unclear, we investigated the role of PKC in HSP72 induction. PKC has been implicated as a mediator of HSP72-induced cytoprotection in the heart; however, its role in renal cell HSP72 induction has not been evaluated. This study therefore constitutes the initial demonstration that heat stress prevents simulated ischemia-induced apoptosis in LLC-PK1 renal tubular cells and distinguishes PKC as an important mediator of this process.

HSP72 induction after heat stress was assessed by exposing renal tubular cells (LLC-PK1) to a 43°C water bath for 1 h. Twenty-four hours later, cells were subjected to Western blot analysis, and a marked increase in HSP72 production was observed. The effect of heat shock on ischemia-induced apoptosis was then evaluated. Simulated ischemia was induced by immersing the entire cellular monolayer in mineral oil, a technique demonstrated to deprive cells of nutrients and restrict metabolite washout (12, 38). Apoptosis was quantified after cell exposure to 1 h of simulated ischemia and 24 h of substrate replacement in the presence or absence of heat preconditioning. The degree of apoptosis was significantly less in cells previously exposed to heat shock. Furthermore, expression of the anti-apoptotic factor Bcl2 (18, 19) decreased in response to simulated ischemia, whereas prior heat preconditioning ameliorated this response. Turman and Rosenfeld (37) suggest that transfected inducible HSP70 does not protect these cells from hypoxic injury (loss of cotransfected luciferase activity); however, our results clearly indicate that heat preconditioning prevents ischemia-induced apoptosis and support previous observations of ischemic cytoprotection (31, 41). The discrepancy between these two studies may be related to differences in the model of injury, the assessment of injury, and/or the expression HSP72 (transfection vs. heat induction).

The mechanisms of HSP72 induction were subsequently investigated by exposing the cells to a PKC inhibitor before thermal stress. PKC inhibition abolished thermal induction of HSP72, and further, prevented the ischemic cytoprotective effect of heat preconditioning. Indeed, cells exposed to PKC inhibition before heat stress demonstrated the same degree of apoptosis as cells subjected to simulated ischemia alone. These findings provide an additional link between HSP72 and cellular protection as well as indicate that PKC is an important mediator of HSP72 induction in renal tubular cells.

The mechanisms by which HSP72 prevents apoptosis appear to be multifactorial. In nonrenal cells, HSP72 has been shown to inhibit early events in the apoptotic cascade, including nuclear factor kappa B activation (9), p38 mitogen-activated protein kinase activation (10), and tumor necrosis factor-alpha -mediated cytotoxicity (14). In renal cells, evidence suggests that novel interactions between HSP72 and the caspase inhibitor Bcl2 may attenuate ischemia-induced apoptosis (41).

Renal tubular cell apoptosis is increasingly recognized as an important mechanism of cell death after ischemia (23, 34). The data presented in this study indicate that thermal induction of HSP72 protects renal tubular cells from ischemia-induced apoptosis through a PKC-dependent mechanism. As we develop a greater understanding of stress proteins, such as HSP72, and their interaction with signal events in the apoptotic cascade, effective strategies to limit or prevent ischemic renal injury may be realized.

Perspectives

Ischemia-induced acute renal failure (ARF) remains an important cause of patient morbidity and mortality. The loss of renal tubular epithelium associated with ARF is attributed to both apoptotic and necrotic cell death, and patient recovery depends, in part, on the protection of renal tubular cells from death. Apoptosis, as a gene-driven process, is uniquely suited to molecular modulation and possible inhibition. As we develop a greater understanding of the intracellular signals that protect against apoptosis, these signals may be manipulated to prevent renal compromise and improve patient outcome. HSPs are a large family of rapidly inducible proteins that provide cellular protection against a variety of noxious stimuli. In this study, we have demonstrated that heat stress and the subsequent induction of HSP72 protect renal tubular cells from ischemia-induced apoptosis. Although the contributions of HSP72 to renal cellular protection are significant, other members of the HSP family, including HSP27, are likely involved. The further elucidation of this family of protective mediators and their intracellular signaling mechanisms will advance the development of therapeutic strategies for the treatment and prevention of ARF.


    FOOTNOTES

Address for reprint requests and other correspondence: K. K. Meldrum, Johns Hopkins Hospital, 600 N. Wolfe St., Dept. of Urology, Marburg 414, Baltimore, MD 21287 (E-mail: kirstan{at}earthlink.net).

The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Received 15 December 2000; accepted in final form 20 March 2001.


    REFERENCES
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

1.   Armstrong, SC, Gao W, Lane JR, and Ganote CE. Protein phosphatase inhibitors calyculin A and fostriecin protect rabbit cardiomyocytes in late ischemia. J Mol Cell Cardiol 30: 61-73, 1998[Web of Science][Medline].

2.   Becker, J, and Craig E. Heat-shock proteins as molecular chaperones. Eur J Biochem 219: 11-23, 1994[Web of Science][Medline].

3.   Brown, M, Upender R, Hightower L, and Renfro J. Thermoprotection of a functional epithelium: heat stress effects on transepithelial transport by flounder renal tubule in primary monolayer culture. Proc Natl Acad Sci USA 89: 3246-3250, 1992[Abstract/Free Full Text].

4.   Buzzard, K, Giaccia A, Killender M, and Anderson R. Heat shock protein 72 modulates pathways of stress-induced apoptosis. J Biol Chem 273: 17147-17153, 1998[Abstract/Free Full Text].

5.   Cleveland, JC, Meldrum DR, Rowland RT, Sheridan BC, Banerjee A, and Harken AH. The obligate role of protein kinase C in mediating clinically accessible cardiac preconditioning. Surgery 120: 345-353, 1996[Web of Science][Medline].

6.   Donnahoo, KK, Meng X, Ayala A, Cain MP, Harken AH, and Meldrum DR. Early kidney TNF-alpha expression mediates neutrophil infiltration and injury following isolated renal ischemia-reperfusion. Am J Physiol Regulatory Integrative Comp Physiol 277: R922-R929, 1999[Abstract/Free Full Text].

7.   Donnahoo, KK, Shames BD, Harken AH, and Meldrum DR. The role of tumor necrosis factor in renal ischemia-reperfusion injury. J Urol 162: 196-203, 1999[Web of Science][Medline].

8.   Emami, A, Schwartz J, and Borkan S. Transient ischemia or heat stress induces a cytoprotectant protein in rat kidney. Am J Physiol Renal Fluid Electrolyte Physiol 260: F479-F485, 1991[Abstract/Free Full Text].

9.   Feinstein, D, Galea E, Aquino D, Li G, Xu H, and Reis D. Heat shock protein 70 suppresses astroglial-inducible nitric-oxide synthase expression by decreasing NFkappa B activation. J Biol Chem 271: 17724-17732, 1996[Abstract/Free Full Text].

10.   Gabai, V, Meriin A, Mosser D, Caron A, Rits S, Shifrin V, and Sherman M. HSP 70 prevents activation of stress kinases. J Biol Chem 272: 18033-18037, 1997[Abstract/Free Full Text].

11.   Gobe, G, Willgoss D, Hogg N, Schoch E, and Endre Z. Cell survival or death in renal tubular epithelium after ischemia-reperfusion injury. Kidney Int 56: 1299-1304, 1999[Web of Science][Medline].

12.   Henry, P, Popescu A, Puceat M, Hinescu ME, and Escande D. Acute simulated ischaemia produces both inhibition and activation of K+ currents in isolated ventricular myocytes. Cardiovasc Res 32: 930-939, 1996[Web of Science][Medline].

13.   Itoh, H, and Tashima Y. The stress (heat shock) proteins. Int J Biochem 23: 1185-1191, 1991[Web of Science][Medline].

14.   Jaattela, M, Wissing D, Kokholm K, Kallunki T, and Egeblad M. HSP 70 exerts its anti-apoptotic function downstream of caspase-3-like proteases. EMBO J 17: 6124-6134, 1998[Web of Science][Medline].

15.   Joannidis, M, Cantley LG, Spokes K, Medina R, Pullman J, Rosen S, and Epstein FH. Induction of heat-shock proteins does not prevent renal tubular injury following ischemia. Kidney Int 47: 1752-1759, 1995[Web of Science][Medline].

16.   Kerr, JF, Wyllie AH, and Currie AR. Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer 26: 239-257, 1972[Web of Science][Medline].

17.   Komatsuda, A, Wakui H, Oyama Y, Imai H, Miura AB, Itoh H, and Tashima Y. Overexpression of the human 72 kDa heat shock protein in renal tubular cells confers resistance against oxidative injury and cisplatin toxicity. Nephrol Dial Transplant 14: 1385-1390, 1999[Abstract/Free Full Text].

18.   Korsmeyer, S, Shutter J, Veis D, Merry D, and Oltavi Z. BCL2/BAX: a rheostat that regulates an antioxidant pathway and cell death. Semin Cancer Biol 4: 327-332, 1993[Web of Science][Medline].

19.   Kromer, G. The proto-oncogene Bcl-2 and its role in regulating apoptosis. Nat Med 3: 614-620, 1997[Web of Science][Medline].

20.   Lee, YJ, Berns CM, Erdos G, Borrelli MJ, Ahn CH, and Corry PM. Effect of 1-(5-isoquinolinesulfonyl)-2-methylpiperazine (H-7) on HSP70 and HSP28 gene expression and thermotolerance development in human colon carcinoma cells. Biochem Pharmacol 48: 2057-2063, 1994[Web of Science][Medline].

21.   Li, Y, Chen C, Ling C, and Li G. Apoptosis in heat-induced cell killing: the protective role of HSP-70 and the sensitization effect of the c-myc gene. Radiat Res 145: 324-330, 1996[Web of Science][Medline].

22.   Lieberthal, W, Koh JS, and Levine JS. Necrosis and apoptosis in acute renal failure. Semin Nephrol 18: 505-518, 1998[Web of Science][Medline].

23.   Lieberthal, W, Menza SA, and Levine JS. Graded ATP depletion can cause necrosis or apoptosis of cultured mouse proximal tubular cells. Am J Physiol Renal Physiol 274: F315-F327, 1998[Abstract/Free Full Text].

24.   Marano, C, Laughlin K, Russo L, and Mullin J. The protein kinase C inhibitor, bisindolylmaleimide, inhibits the TPA-induced but not the TNF-induced increase in LLC-PK1 transepithelial permeability. Biochem Biophys Res Commun 209: 669-676, 1995[Web of Science][Medline].

25.   Meldrum, DR. Tumor necrosis factor in the heart. Am J Physiol Regulatory Integrative Comp Physiol 274: R577-R595, 1998[Abstract/Free Full Text].

26.   Meldrum, DR, Cleveland JC, Mitchell MB, Sheridan BC, Robertson F, Harken AH, and Banerjee A. Protein kinase C mediates Ca2+ induced cardioadaptation to ischemia-reperfusion injury. Am J Physiol Regulatory Integrative Comp Physiol 271: R1718-R1726, 1996.

27.   Meldrum, DR, Cleveland JC, Sheridan BC, Meng X, Robertson F, Cain BS, Harken AH, and Banerjee A. Protein kinase C isoform diversity in preconditioning. J Surg Res 69: 183-187, 1997[Web of Science][Medline].

28.   Meldrum, DR, Mitchell MB, Banerjee A, and Harken AH. Cardiac preconditioning: induction of endogenous tolerance to ischemia-reperfusion injury. Arch Surg 128: 1208-1211, 1993[Abstract/Free Full Text].

29.   Morita, K, Wakui H, Komatsuda A, Ohtani H, Miura AB, Itoh H, and Tashima Y. Induction of heat-shock proteins HSP73 and HSP90 in rat kidneys after ischemia. Ren Fail 17: 405-419, 1995[Web of Science][Medline].

30.   Mosser, D, Caron A, Bourget L, Denis-Larose C, and Massie B. Role of the human heat shock protein hsp70 in protection against stress-induced apoptosis. Mol Cell Biol 17: 5317-5327, 1997[Abstract].

31.   Perdrizet, G, Kaneko H, Buckley T, Fishman M, Pleau M, Bow L, and Schweizer R. Heat shock and recovery protects renal allografts from warm ischemic injury and enhances HSP72 production. Transplant Proc 25: 1670-1673, 1993[Web of Science][Medline].

32.   Rehring, TF, Shapiro JI, Cain BS, Meldrum DR, Cleveland JC, Harken AH, and Banerjee A. Mechanisms of pH preservation during global ischemia in preconditioned rat heart: roles for PKC and NHE. Am J Physiol Heart Circ Physiol 275: H805-H813, 1998[Abstract/Free Full Text].

33.   Schawalder, A, Oertli B, Beck-Schimmer B, and Wuthrich RP. Regulation of hyaluronan-stimulated VCAM-1 expression in murine renal tubular epithelial cells. Nephrol Dial Transplant 14: 2130-2136, 1999[Abstract/Free Full Text].

34.   Schumer, M, Colombel MC, Sawczuk IS, Gobe G, Connor J, O'Toole KM, Olsson CA, Wise GJ, and Buttyan R. Morphologic, biochemical, and molecular evidence of apoptosis during the reperfusion phase after brief periods of renal ischemia. Am J Pathol 140: 831-838, 1992[Abstract].

35.   Steller, H. Mechanisms and genes of cellular suicide. Science 267: 1445-1449, 1995[Abstract/Free Full Text].

36.   Thompson, CB. Apoptosis in the pathogenesis and treatment of disease. Science 267: 1456-1462, 1995[Abstract/Free Full Text].

37.   Turman, MA, and Rosenfeld SL. Heat shock protein 70 overexpression protects LLC-PK1 tubular cells from heat shock but not hypoxia. Kidney Int 55: 189-197, 1999[Web of Science][Medline].

38.   Vanheel, B, Leybaert L, De Hemptinne A, and Leusen I. Simulated ischemia and intracellular pH in isolated ventricular muscle. Am J Physiol Cell Physiol 257: C365-C376, 1989[Abstract/Free Full Text].

39.   Van-Why, S, Hildebrandt F, Ardito T, Mann A, Siegel N, and Kashgarian M. Induction and intracellular localization of HSP-72 after renal ischemia. Am J Physiol Renal Fluid Electrolyte Physiol 263: F769-F775, 1992[Abstract/Free Full Text].

40.   Wakui, H, Komatsuda A, and Miura AB. Heat-shock proteins in animal models for acute renal failure. Ren Fail 17: 641-649, 1995[Web of Science][Medline].

41.   Wang, Y, Knowlton AA, Christensen TG, Shih T, and Borkan SC. Prior heat stress inhibits apoptosis in adenosine triphosphate-depleted renal tubular cells. Kidney Int 55: 2224-2235, 1999[Web of Science][Medline].

42.   Wang, YH, and Borkan SC. Prior heat stress enhances survival of renal epithelial cells after ATP depletion. Am J Physiol Renal Fluid Electrolyte Physiol 270: F1057-F1065, 1996[Abstract/Free Full Text].

43.   Welch, W. Mammalian stress response: cell physiology, structure/function of stress proteins, and implications for medicine and disease. Physiol Rev 72: 1063-1081, 1992[Free Full Text].

44.   Yamashita, N, Hoshida S, Nishida M, Igarashi J, Aoki K, Hori M, Kuzuya T, and Tada M. Time course of tolerance to ischemia-reperfusion injury and induction of heat shock protein 72 by heat stress in the rat heart. J Mol Cell Cardiol 29: 1815-1821, 1997[Web of Science][Medline].

45.   Yuan, CM, Bohen EM, Musio F, and Carome MA. Sublethal heat shock and cyclosporine exposure produce tolerance against subsequent cyclosporine toxicity. Am J Physiol Renal Fluid Electrolyte Physiol 271: F571-F578, 1996[Abstract/Free Full Text].

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