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Am J Physiol Regul Integr Comp Physiol 287: R742-R748, 2004. First published May 27, 2004; doi:10.1152/ajpregu.00041.2004
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APPETITE, OBESITY, DIGESTION, AND METABOLISM

Vagal cooling and concomitant portal norepinephrine infusion do not reduce net hepatic glucose uptake in conscious dogs

Sylvain Cardin, Michael J. Pagliassotti, Mary Courtney Moore, Dale S. Edgerton, Margaret Lautz, Ben Farmer, Doss W. Neal, and Alan D. Cherrington

Department of Molecular Physiology and Biophysics and Diabetes Research and Training Center, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-0615

Submitted 20 January 2004 ; accepted in final form 25 May 2004


   ABSTRACT
TOP
 ABSTRACT
MATERIALS AND METHODS
 RESULTS
 DISCUSSION
 GRANTS
 REFERENCES
 
We examined the role of efferent neural signaling in regulation of net hepatic glucose uptake (NHGU) in two groups of conscious dogs with hollow perfusable coils around their vagus nerves, using tracer and arteriovenous difference techniques. Somatostatin, intraportal insulin and glucagon at fourfold basal and basal rates, and intraportal glucose at 3.8 mg·kg–1·min–1 were infused continuously. From 0 to 90 min [period 1 (P1)], the coils were perfused with a 37°C solution. During period 2 [P2; 90–150 min in group 1 (n = 3); 90–180 min in group 2 (n = 6)], the coils were perfused with –15°C solution to eliminate vagal signaling, and the coils were subsequently perfused with 37°C solution during period 3 (P3). In addition, group 2 received an intraportal infusion of norepinephrine at 16 ng·kg–1·min–1 during P2. The effectiveness of vagal suppression was demonstrated by the increase in heart rate during P2 (111 ± 17, 167 ± 16, and 105 ± 13 beats/min in group 1 and 71 ± 6, 200 ± 11, and 76 ± 6 beats/min in group 2 during P1–P3, respectively) and by prolapse of the third eyelid during P2. Arterial plasma glucose, insulin, and glucagon concentrations; hepatic blood flow; and hepatic glucose load did not change significantly during P1–P3. NHGU during P1-P3 was 2.7 ± 0.4, 4.1 ± 0.6, and 4.0 ± 1.2 mg·kg–1·min–1 in group 1 and 5.0 ± 0.9, 5.6 ± 0.7, and 6.1 ± 0.9 mg·kg–1·min–1 in group 2 (not significant among periods). Interruption of vagal signaling with or without intraportal infusion of norepinephrine to augment sympathetic tone did not suppress NHGU during portal glucose delivery, suggesting the portal signal stimulates NHGU independently of vagal efferent flow.

vagus nerve; efferent neural transmission

NET HEPATIC GLUCOSE UPTAKE (NHGU) and glycogen synthesis are enhanced when glucose is delivered in the hepatic portal vein compared with peripheral venous delivery (1, 23, 34). This increase in NHGU in response to portal glucose delivery is related to the negative arterial-portal glucose gradient (arterial glucose concentration less than that in the portal vein) that develops in the postprandial period and during portal glucose infusion and has been termed the "portal signal" (35). Total surgical hepatic denervation abolishes the stimulation of NHGU induced by the portal signal (2), indicating that the central nervous system (CNS) is likely to be involved in this phenomenon.

Neurophysiological data provide evidence for CNS involvement in the portal signal. The afferent firing rate in the hepatic branch of the vagus nerve is inversely related to the portal vein glucose concentration (27). Similarly, glucose-sensitive neurons in the lateral hypothalamus decrease their firing rates when glucose is injected in the portal vein, a phenomenon that is mediated by noradrenergic mechanisms (41). Infusion of glucose in the portal vein was also associated with a decrease in the firing rate of the hepatic branch of the splanchnic (sympathetic) nerve (32) and an increase in the firing rate of the pancreatic branch of the vagus nerve (25). To our knowledge, the efferent firing rate in the hepatic branch of the vagus nerve has not been assessed during portal glucose administration, but efferent hepatic vagal activity is enhanced by hyperglycemia brought about by intravenous glucose injection (33).

Therefore, we hypothesized that the portal signal brings about its effect as a result of suppression of afferent firing in the hepatic branch of the vagus nerves, with a resulting enhancement of efferent hepatic parasympathetic activity and an inhibition of the efferent firing rate in the hepatic branch of the splanchnic (sympathetic) nerve (32). Parasympathetic stimulation enhances hepatic glucose uptake, glycogen synthase activity, and glycogen storage in the perfused liver (40), and the portal signal brings about all of these effects in vivo (34). Thus we initially postulated that the effects of the parasympathetic nervous system are dominant. To examine the role of the parasympathetic nervous system, we cooled the vagus nerves of conscious dogs during portal glucose delivery, thus interrupting the efferent signal. Cooling would also interrupt afferent transmission, but this should be equivalent to a maximal stimulus, since portal glucose delivery induces a fall in the firing rate of the vagal afferents (27). When an initial series of studies indicated that vagal cooling did not suppress NHGU, we expanded the experimental design to examine the role of sympathetic signaling in bringing about the effects of the portal signal. In other words, we postulated that the suppression of sympathetic input to the liver during portal glucose administration removes a tonic block to NHGU. To mimic augmentation of sympathetic tone to the liver, we infused norepinephrine (NE) in the hepatic portal circulation at a low rate during the cooling period in a second group of dogs.


   MATERIALS AND METHODS
TOP
 ABSTRACT
 MATERIALS AND METHODS
RESULTS
 DISCUSSION
 GRANTS
 REFERENCES
 
Animal Care

Experiments were conducted on conscious, mongrel dogs of either sex weighing 23.1 ± 0.8 kg. The animals were fed meat and chow one time daily (KalKan, Vernon, CA and Purina Lab Canine Diet No. 5006, St. Louis, MO; 34% protein, 46% carbohydrate, 14.5% fat, and 5.5% fiber based on dry weight). The animals were housed in a facility that met the standards of the American Association for the Accreditation of Laboratory Animal Care International, and the protocols were approved by the Institutional Animal Care and Use Committee.

Surgical Procedures

Before the experiment (2 wk), all dogs underwent a laparotomy under general anesthesia, and infusion catheters were placed in a splenic and a jejunal vein for intraportal infusions. Catheters for blood sampling were inserted in the left common hepatic vein, the portal vein, and the femoral artery, as previously described (23). Doppler flow probes (group 1, n = 3; Instrumentation Laboratories, Baylor University, Houston, TX) or ultrasonic flow probes (group 2, n = 6; Transonic Systems, Ithaca, NY) were placed around the hepatic artery and the portal vein to determine hepatic blood flow (HBF). The flow probe leads and the catheters were placed in subcutaneous pockets before closure of the abdominal skin. Stainless steel cooling coils, with Silastic (Dow Corning, Midland, MI) extension tubing attached, were placed around the vagus nerves in the neck of all dogs, as described previously (20). Each dog was used for an experiment only if it had a leukocyte count <18,000/mm3, a hematocrit >35%, a good appetite, and normal stools.

On the day of the study, the proximal ends of the infusion and sampling catheters and the flow probe leads were exposed and prepared as previously described (24). The splenic and jejunal catheters were used for intraportal infusion of porcine insulin (Eli Lilly, Indianapolis, IN), glucagon (GlucaGen, Novo Nordisk, Denmark), NE (Sigma, St. Louis, MO), 20% dextrose (Baxter, Deerfield, IL), and p-aminohippuric acid (PAH; Sigma). Angiocaths (Deseret Medical, Becton-Dickinson, Sandy, UT) were inserted percutaneously in superficial leg veins for [3-3H]glucose (DuPont-NEN, Boston, MA), indocyanine green dye (ICG; Sigma), somatostatin (Bachem, Torrance, CA), and 50% dextrose (Abbott Laboratories, North Chicago, IL) infusions. The Silastic tubes connected to the cooling coils were removed from their subcutaneous pockets and joined to inflowing lines from the cooling bath and outflowing lines to a collection reservoir (20).

Experimental Design

The dogs were fasted 42 h before study because this brings hepatic glycogen to a stable minimum, ~20 mg/g (15, 36). For group 1, the experiment consisted of a tracer, PAH and ICG equilibration period (–140 min to –40 min), a 40-min period for basal sampling (–40 min to 0 min), and a 210-min experimental period. At –140 min, a primed (36 µCi), continuous (0.3 µCi/min) peripheral infusion of [3-3H]glucose and a continuous infusion of ICG (4 µg·kg–1·min–1) were started and continued throughout the experiment. To equilibrate PAH in the blood, a peripheral infusion of PAH (0.4 mg·kg–1·min–1) was started at –140 min and stopped at 0 min. At time 0, constant infusions of several solutions were started, and they were continued throughout the experimental period. Somatostatin (0.8 µg·kg–1·min–1) was infused via a peripheral vein to suppress endogenous insulin and glucagon secretion. Insulin (1.2 mU·kg–1·min–1) and glucagon (0.65 ng·kg–1·min–1) were infused intraportally at rates designed to achieve arterial concentrations three- to fourfold basal and basal, respectively. Glucose (20% dextrose) was infused portally at 3.8 ± 0.1 mg·kg–1·min–1. PAH (mixed with the portal glucose infusate so that the PAH delivery rate would be 0.4 mg·kg–1·min–1) was administered to assess mixing of the infused glucose with blood in the portal and hepatic veins, as described previously (34). In addition, a primed continuous peripheral infusion of 50% dextrose was begun so that the hepatic glucose load could be quickly clamped at twofold basal. The experimental period was divided into a 90-min period during which the vagal coils were infused with solution at body temperature (37°C; 0–90 min, period 1), a 60-min period (90–150 min, period 2) of vagal cooling (–15°C in the bath), and a subsequent period (150–210 min, period 3) when the coils were again perfused with a body temperature solution. For group 2, study conditions were identical to those in group 1, with three exceptions. First, the glucagon infusion during the entire experimental period was 0.5 ng·kg–1·min–1 because the infusion rate in group 1 appeared to result in concentrations slightly above basal for 42-h-fasted dogs. This might have tended to reduce NHGU in group 1, but it should not have affected the overall conclusion (16), since the same rate was used during all three periods. Second, periods 2 and 3 were lengthened to 90 min (i.e., period 2 = 90–180 min and period 3 = 180–270 min) to make them equivalent in length to period 1. Third, simultaneously with vagal cooling during period 2, NE was infused intraportally at 16 ng·kg–1·min–1 to augment hepatic sympathetic tone; the NE infusion was stopped concomitantly with the cessation of vagal cooling at 180 min.

The effectiveness of the cooling-induced blockade of parasympathetic signaling was verified by measuring the heart rate (primarily under vagal control in the basal state and during normal fluctuations of blood pressure; see Refs. 21 and 38), verifying a decrease in rate and depth of respirations, and observing bilateral Horner's syndrome (8).

Processing and Analysis of Samples

Plasma glucose, insulin, glucagon, NE, and cortisol and blood lactate were measured as described elsewhere (7, 34). PAH concentrations were also measured in perchloric acid-deproteinized blood, as previously described (34).

Calculations

Mixing of the infused glucose with the portal vein blood was assessed by comparing the recovery of PAH (which was mixed with the portal glucose infusate) in the portal and hepatic veins with the PAH infusion rate (23, 34). An experiment was deleted from the database if poor mixing, as defined previously (34), was observed at more than one of the four time points in the portal glucose infusion phase. In this study, 15 dogs were used; six were not included because of catheter failure or poor mixing. In the animals that were retained, the ratio of PAH recovery in the portal vein to the PAH infusion rate was 1.1 ± 0.2, whereas the ratio of PAH recovery in the hepatic vein to the PAH infusion rate was 0.9 ± 0.2 (a ratio of 1.0 would represent perfect mixing). When a dog was retained in the database, all of the points were used whether they were mixed or not, because mixing errors occur randomly.

HBF was calculated by two methods, Doppler or ultrasonic flow probes and dye extraction (34). The results obtained with flow probes and ICG extraction were not significantly different, but the data shown are those obtained with the flow probes, because their use did not require an assumption regarding the distribution of arterial and portal contributions to HBF.

The rate of substrate delivery to the liver, or hepatic substrate load; net hepatic substrate balance; net hepatic fractional glucose extraction; and nonhepatic glucose uptake were calculated as described previously (37). Glucose data were calculated with both direct and indirect methods (37). There were no significant differences with the results of the two methods of calculation, but the results reported here utilize the indirect calculation, unless specified otherwise, to minimize any error resulting from incomplete mixing of the glucose infusate in the portal circulation. Plasma glucose values were converted to wholeblood glucose values for all calculations using correction factors derived from previous studies in our laboratory (17, 18, 34). Hepatic sinusoidal insulin and glucagon concentrations (37) and hepatic NE spillover (9) were calculated as previously described. Arterial cortisol concentrations and portal vein concentrations of insulin, glucagon, and NE were not obtained in group 1.

The rates of glucose appearance (Ra) and disappearance (Rd) were calculated with a two-compartment model, using dog parameters (11, 22). The portal glucose infusion rate was multiplied by 1 – hepatic fractional glucose extraction and added to the peripheral glucose infusion rate; the combined rate was subtracted from total Ra to yield endogenous Ra (EndoRa).

Data are expressed as means ± SE. Data reported for periods 1–3 are the means of the three sampling points in the last 30 min of each period, thus ensuring that steady-state conditions existed. Statistical comparisons among periods were made using repeated-measures ANOVA (SigmaStat; SPSS, Chicago, IL). Post hoc analysis was performed using the Holm-Sidak procedure for pairwise comparisons. Significance was presumed at P < 0.05.


   RESULTS
TOP
 ABSTRACT
 MATERIALS AND METHODS
 RESULTS
DISCUSSION
 GRANTS
 REFERENCES
 
Group 1 (Vagal Cooling Without NE Infusion)

Cardiovascular parameters and HBF. Blockade of vagal firing was confirmed by a significantly (P < 0.05) greater heart rate (167 ± 16 beats/min) during period 2, compared with the other periods (basal: 121 ± 17; period 1: 111 ± 17; and period 3: 105 ± 13 beats/min). In addition, all dogs evidenced prolapse of the third eyelid (Horner's sign) and a deepening and slowing of respirations during period 2, and this was reversed during period 3. There was no significant change in blood pressure during the basal periods and periods 1–3 (mean arterial pressures: 94 ± 5, 99 ± 12, 103 ± 14, and 94 ± 17 mmHg, respectively). Similarly, HBF did not change significantly in any of the periods (29 ± 2, 27 ± 1, 29 ± 2, and 30 ± 2 ml·kg–1·min–1 during the basal period and periods 1–3, respectively).

Hormone concentrations. Insulin concentrations increased approximately threefold between the basal and experimental period, but there were no significant changes over time in glucagon and NE. Moreover, the concentrations for insulin, glucagon, and NE did not differ among periods 1–3 (Table 1).


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  		Table 1. Arterial plasma hormone concentrations in group 1

 
Glucose and lactate metabolism. Arterial blood glucose did not differ among periods 1–3, averaging approximately twofold basal (Table 2). The arterial-portal vein glucose gradient was negative during the experimental period and did not differ significantly among periods 1–3. The hepatic glucose load increased approximately twofold basal during periods 1–3 and did not differ among these periods. The dogs switched from net hepatic glucose output to NHGU with the onset of the experimental period, with no significant difference in rates among periods 1–3 (Table 2). Net hepatic fractional extraction of glucose did not differ significantly among periods 1–3 (0.07 ± 0.01, 0.10 ± 0.02, and 0.10 ± 0.03, respectively). The total glucose infusion rates and nonhepatic glucose uptakes were statistically similar among periods 1–3.


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  		Table 2. Glucose and lactate data in group 1

 
Arterial blood lactate concentrations increased over basal during periods 1–3 but did not differ among the three periods. The livers of the animals shifted from net lactate uptake to net output during the experimental period. Net hepatic lactate output was greatest during period 1, but it did not differ significantly between periods 2 and 3.

Group 2 (Vagal Cooling Plus NE Infusion)

Heart rate and HBF. Blockade of vagal firing was evident based on the significant (nearly 3-fold) increase in heart rate during period 2 (Table 3). As in group 1, all dogs evidenced prolapse of the third eyelid (Horner's sign) and a deepening and slowing of respirations during period 2, which was reversed during period 3. Hepatic artery blood flow increased significantly and portal vein blood flow decreased significantly after the basal period, concomitant with the initiation of the somatostatin infusion. Neither hepatic artery nor portal vein blood flow changed significantly after that time (Table 3).


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  		Table 3. Heart rate and hepatic blood flow in group 2

 
Hormone concentrations. Arterial and hepatic sinusoidal plasma insulin concentrations were increased three- to fourfold above basal during periods 1–3, respectively [Table 4; not significant (NS) among experimental periods]. Arterial and sinusoidal plasma glucagon concentrations remained at basal throughout the experiments (Table 4).


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  		Table 4. Plasma hormone data in group 2

 
There were no significant differences in arterial plasma NE levels among the test periods (Table 4). NE infusion in the portal vein increased the portal vein plasma NE level so that the sinusoidal concentration rose fivefold during period 2, but the concentration fell below basal during period 3. Hepatic NE spillover increased ~60% during NE infusion and then returned to basal after cessation of the infusion. As shown in our previous work (20), vagal cooling created a threefold increase in the arterial plasma cortisol level; the cortisol levels decreased with the cessation of vagal cooling.

Glucose metabolism. Arterial blood glucose increased approximately twofold, from 75 ± 2 mg/dl in the basal period to 170 ± 3, 167 ± 2, and 162 ± 2 mg/dl during the last 30 min of periods 1, 2, and 3, respectively (NS among experimental periods; Fig. 1). Because of the infusion of glucose in the portal vein during the experimental periods, the arterial-portal difference shifted from a positive to a negative value in periods 1, 2, and 3 (NS among periods 1–3; Table 5).



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  		Fig. 1. Arterial blood glucose, hepatic glucose load, net hepatic glucose uptake, and net hepatic fractional glucose extraction in 6 conscious dogs (group 2) studied under basal conditions and during 3 consecutive 90-min experimental periods (periods 1–3) during which somatostatin, intraportal insulin and glucagon (4-fold basal and basal rates, respectively), intraportal glucose (3.8 mg·kg–1·min–1), and peripheral glucose infusions were administered. During period 2, the vagus nerve was cooled to halt transmission of impulses via the nerve, and norepinephrine (NE) was infused intraportally to simulate sympathetic activation of the liver. Period 3 followed the discontinuance of vagal cooling and NE infusion. There were no significant differences among periods 1–3.

 

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  		Table 5. A-P blood glucose gradient, glucose infusion rate, nonhepatic glucose uptake, and lactate data in group 2

 
The hepatic glucose load increased approximately twofold above basal in periods 1, 2, and 3, respectively, and did not differ among the periods (Fig. 1).

In the basal state, net hepatic glucose output was 1.9 ± 0.3 mg·kg–1·min–1 (Fig. 1). The combination of increases in the hepatic glucose load and the insulin level as well as the presence of the portal signal caused the liver to exhibit a net glucose uptake of 5.0 ± 0.9 mg·kg–1·min–1 (using the indirect calculation) in period 1. Interruption of vagal firing by vagal cooling and augmentation of sympathetic activity by hepatic portal delivery of NE did not decrease NHGU (5.6 ± 0.7 mg·kg–1·min–1). Likewise, there was no significant change in NHGU when vagal cooling and NE infusion were stopped (6.1 ± 0.9 mg·kg–1·min–1). The values for NHGU using the direct calculation were 4.4 ± 1.2, 4.2 ± 0.7, and 5.6 ± 0.8 mg·kg–1·min–1 during periods 1–3, respectively (NS compared with the values from the indirect calculation). The fractional extraction of glucose by the liver was also unaffected by vagal cooling (0.08 ± 0.01, 0.11 ± 0.02, and 0.11 ± 0.03 in periods 1, 2, and 3, respectively, Fig. 1).

Glucose EndoRa declined from 2.5 ± 0.2 mg·kg–1·min–1 in the basal period to 0.5 ± 0.3 mg·kg–1·min–1 under the influence of hyperglycemia, hyperinsulinemia, and the portal signal. There were no statistical differences in EndoRa during periods 1–3. Glucose Rd showed a tendency to increase progressively during periods 1–3 (13.5 ± 2.8, 16.3 ± 3.7, and 17.8 ± 5.6 mg·kg–1·min–1, respectively), but there was no statistically significant change over time (P = 0.2). Moreover, nonhepatic glucose uptake did not change significantly during periods 1–3 (Table 5).

Lactate metabolism. The arterial blood lactate level rose approximately threefold above basal during periods 1–3 and did not differ among the periods (Table 5). The liver switched from net hepatic lactate uptake to output at the onset of the experimental periods, and the rate did not differ among the periods.


   DISCUSSION
TOP
 ABSTRACT
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
GRANTS
 REFERENCES
 
Group 1 (Vagal Cooling Without NE Infusion)

In the current studies, NHGU was initially stimulated by hyperinsulinemia, hyperglycemia, and the presence of the portal signal. When vagus nerve activity was then blocked by cooling (group 1), NHGU was not inhibited. Although group 1 was composed of only three dogs, there appears to be little possibility of a type II error. NHGU increased modestly, rather than decreased, between periods 1 and 2 in every animal and did not increase further during period 3.

If the portal signal is transmitted to the CNS via a suppression of afferent firing in the hepatic branch of the vagus, as neurophysiological data strongly suggest (29–31), then blocking the afferent signaling by vagal cooling should have created a maximal stimulus. Thus our data suggest that efferent parasympathetic signaling is not required for enhancement of NHGU in response to the portal signal. However, there are possible alternatives to this conclusion. First, the efferent signal could be transmitted via vagal parasympathetic fibers not blocked by cooling the nerve. This possibility seems unlikely, given that the heart rate increased significantly during cooling, Horner's sign was present, and respirations changed as expected. We have previously shown (20) that vagal cooling bath temperatures of –13 to –15°C produce temperatures of ~2°C at the nerve, which were sufficient to halt vagal signaling in the dog (8) and cat (13). Injection of atropine (known to maximally stimulate heart rate) is not able to produce an additional increase in heart rate over cooling at this temperature (20). Second, the stimulation of NHGU could result from a local parasympathetic reflex within the hepatoportal region, without evoking a centrally mediated response. A local cholinergic reflex was suggested by work in the isolated, perfused rat liver (43). However, the existence of an intrahepatic reflex does not rule out the possibility that a centrally mediated response forms a component of the portal signal. That complete surgical denervation of the liver blunted NHGU during portal glucose infusion in the dog (2) is consistent with central mediation of the portal signal. Additionally, the ability of the portal signal to enhance insulin secretion (12) is apparently mediated by reflex stimulation of the firing rate in the pancreatic branch of the vagus nerve (25, 46, 47), and this stimulation is prevented by sectioning the hepatic branch of the vagus (25, 47), consistent with central control. Thus it is possible that both local and centrally mediated mechanisms could work together to bring about the full response to the portal signal. Finally, there is the possibility that cooling the vagus produced offsetting signals by halting both parasympathetic and sympathetic signaling. As mentioned previously, portal glucose delivery results in a fall in the efferent firing rate in the hepatic branch of the splanchnic (sympathetic) nerve, and the fall is prevented in animals that undergo sectioning of the hepatic branch of the vagus nerve (32). Thus it appears that a fall in afferent parasympathetic signaling in response to entry of glucose in the portal vein results in an enhancement of efferent parasympathetic firing and a concomitant suppression of hepatic sympathetic tone, with the two efferent signals cooperating to enhance NHGU. Vagal cooling completely halts the firing in the nerve (13); although afferent firing diminishes in an inverse relationship with the portal vein glucose concentration, it apparently never ceases altogether (29). Thus we were concerned that we might have induced a greater suppression of sympathetic input to the liver than normally occurs, offsetting any impact of the reduction in parasympathetic efferent signaling on NHGU. Therefore, we conducted further studies in which study conditions were essentially the same as those in the first group, except that a low-dose intraportal infusion of NE, designed to target the liver without raising systemic NE concentrations, was used to simulate maintenance of hepatic sympathetic tone.

Group 2 (Vagal Cooling Plus NE Infusion)

The combination of vagal cooling and low-dose intraportal NE infusion in the presence of the portal signal also had no suppressive effect on NHGU. Although this suggests that our revised hypothesis was incorrect, it does not completely disprove it. Infusion of NE in the portal vein might not have adequately simulated maintenance of sympathetic signaling to the liver. First, infusion of NE in the blood may not have positioned it to contact receptors located in the hepatic parenchyma of the dog liver (3). However, portal vein NE administration in the isolated, perfused rat liver closely mimics the effects of hepatic nerve stimulation on glucose and lactate output (14, 44). Moreover, both in the sheep (4) and the dog (7), intraportal NE infusion has been used in studying the effects of a selective rise in sinusoidal NE on hepatic glucose production. Second, this rate may not have been sufficient to mimic normal sympathetic tone. In a previous study, hepatic NE spillover fell modestly but significantly, from 1.6 ± 0.4 to 1.1 ± 0.3 ng·kg–1·min–1, during portal glucose delivery (19), and we chose an NE infusion rate calculated to maintain but not exceed a basal rate of spillover. In this we were successful. The rate of hepatic NE spillover evident in this study, 1.3 ± 0.4 ng·kg–1·min–1, is virtually identical to that observed in normal dogs studied in the basal state and during moderate-intensity exercise (10) and is substantially less than that observed in the dog during heavy exercise (9). Higher levels of intraportal NE infusion (50 ng·kg–1·min–1, elevating the hepatic sinusoidal NE concentration to 4,100 pg/ml) markedly stimulated hepatic glycogenolysis (7), and our infusion rate was chosen to avoid this direct effect on the liver, as well as potential indirect effects resulting from NE action on peripheral tissues. Finally, it is possible that a neurotransmitter other than NE, e.g., serotonin (26, 28), is normally involved in a sympathetic component of the efferent limb of the portal signal. Nevertheless, if the portal signal normally causes a coordinated change in parasympathetic and sympathetic signaling, it is unlikely that blocking part of the efferent signaling (the rise in vagal efferent firing) while leaving the sympathetic limb intact would have resulted in a full response to the portal signal. Certainly, the intraportal infusion of {alpha}- and {beta}-adrenergic receptor antagonists did not enhance NHGU during hyperinsulinemia and hyperglycemia (achieved via peripheral glucose infusion) in the conscious dog (42).

Role of Parasympathetic Innervation in Liver Glucose Metabolism

A large body of data indicates that the parasympathetic nervous system is involved in the regulation of NHGU and hepatic glucose metabolism (reviewed in Ref. 5). In vitro evidence is especially convincing. Stimulation of the distal cut end of the vagus nerve increases glycogen synthase activity and glycogen synthesis independent of changes in insulin (39). In a perfused liver preparation under hyperglycemic and hyperinsulinemic conditions, the addition of ACh to the perfusate increased NHGU, whereas the addition of atropine decreased it (43). However, both nerve stimulation and ACh administration may have produced a supraphysiological stimulus. In addition, they represented an isolated parasympathetic stimulus to the liver, and it may be that, when the sympathetic nervous system is functional, it dominates in the regulation of hepatic glucose metabolism.

In vivo data regarding parasympathetic regulation of hepatic glucose metabolism are less abundant than in vitro data. Using conscious dogs, Chap et al. (6) found that NHGU in response to ingestion of a glucose load was reduced 44% by portal infusion of atropine. Atropine also altered the rate of portal blood flow and the absorption of the glucose load, however, raising the question of whether its effects on NHGU might have been indirect rather than a direct effect of muscarinic blockade. Shiota et al. (42) showed that, during a hyperglycemic, hyperinsulinemic clamp in the conscious dog, ACh infusion in the hepatic portal vein caused an increase in NHGU. It is important to note that, in this experiment, the infusion of ACh increased hepatic arterial blood flow and, as a result, the hepatic glucose load. Nevertheless, the fractional extraction of glucose by the liver increased, indicating that hepatic glucose uptake was stimulated even when the increase in the hepatic glucose load was taken into account. It remains possible, however, that the increase in fractional extraction resulted from the same stimulus as that causing the increased HBF. Moreover, both the studies of Chap et al. (6) and Shiota et al. (42) suffer from the fact that it is difficult to be sure that neurotransmitters or receptor blocking agents reach their physiological targets when they are delivered via the bloodstream, as previously noted in regard to the NE infusion in the current study. In regard to surgical interruption of vagal signaling, it is noteworthy that rats with isolated hepatic cholinergic denervation exhibited significantly less hepatic glycogen in the fed state than did sham-operated controls (45). However, the rats were studied 4 wk postoperatively. It is not clear that these findings in a chronically denervated model are relevant in a more acute setting, such as changes in neural firing in response to feeding. Thus, although it seems clear that the parasympathetic nervous system can enhance hepatic glucose uptake and storage in vitro, neither the published literature nor our current findings provide compelling evidence that the hepatic parasympathetic efferent innervation plays a major role in acute regulation of NHGU in vivo.

In conclusion, the present data do not confirm the hypothesis that the portal signal is mediated by a decrease in afferent vagal firing stimulating an increase in parasympathetic efferent stimuli from the CNS to the liver. Intraportal NE infusion also failed to blunt NHGU, but this may have been because it did not adequately simulate maintenance of sympathetic tone. The current data thus suggest that the enhancement of NHGU by the portal signal is mediated by a local intrahepatic reflex or another neurotransmitter. We cannot rule out, however, that suppression of sympathetic stimuli to the liver plays a part in bringing about the portal signal.


   GRANTS
TOP
 ABSTRACT
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
 GRANTS
REFERENCES
 
This work was supported by National Institutes of Health Grants R01-DK-43706 and SP-60-AM-20593.


   ACKNOWLEDGMENTS
 
We appreciate the assistance of Jon Hastings and the Diabetes Research and Training Center Hormone Core (Wanda Snead, Angelina Penaloza, and Eric Allen) in these studies.

Current addresses: S. Cardin, Wellstat Therapeutics, Gaithersburg, MD 20878-1301; and M. J. Pagliassotti, Department of Food Science and Human Nutrition, Colorado State University, Fort Collins, CO 80523.


   FOOTNOTES
 

Address for reprint requests and other correspondence: M. C. Moore, 702 Light Hall, Dept. of Molecular Physiology and Biophysics, Vanderbilt Univ. School of Medicine, Nashville, TN 37232–0615 (E-mail: genie.moore{at}vanderbilt.edu)

The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.


   REFERENCES
TOP
 ABSTRACT
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
 GRANTS
 REFERENCES
 

  1. Adkins BA, Myers SR, Hendrick GK, Stevenson RW, Williams PE, and Cherrington AD. Importance of the route of intravenous glucose delivery to hepatic glucose balance in the conscious dog. J Clin Invest 79: 557–565, 1987.[Web of Science][Medline]
  2. Adkins-Marshall BA, Pagliassotti MJ, Asher JR, Connolly CC, Neal DW, Williams PE, Myers SR, Hendrick GK, Adkins RB Jr, and Cherrington AD. Role of hepatic nerves in response of liver to intraportal glucose delivery in dogs. Am J Physiol Endocrinol Metab 262: E679–E686, 1992.[Abstract/Free Full Text]
  3. Akiyoshi H, Gonda T, and Terada T. A comparative histochemical and immunohistochemical study of aminergic, cholinergic and peptidergic innervation in rat, hamster, guinea pig, dog and human livers. Liver 18: 352–359, 1998.[Web of Science][Medline]
  4. Apatu RS and Barnes RJ. Release of glucose from the liver of fetal and postnatal sheep by portal vein infusion of catecholamines or glucagon. J Physiol 436: 449–468, 1991.[Abstract/Free Full Text]
  5. Bollen M, Keppens S, and Stalmans W. Specific features of glycogen metabolism in the liver. Biochem J 336: 19–31, 1998.
  6. Chap Z, Ishida T, Chou J, Lewis R, Hartley C, Entman M, and Field JB. Effects of atropine and gastric inhibitory polypeptide on hepatic glucose uptake and insulin extraction in conscious dogs. J Clin Invest 76: 1174–1181, 1985.[Web of Science][Medline]
  7. Chu CA, Sindelar DK, Neal DW, Allen EJ, Donahue EP, and Cherrington AD. Effect of a selective rise in sinusoidal norepinephrine on HGP is due to an increase in glycogenolysis. Am J Physiol Endocrinol Metab 274: E162–E171, 1998.[Abstract/Free Full Text]
  8. Chung SA, Valdez DT, and Diamant NE. Adrenergic blockage does not restore the canine gastric migrating motor complex during vagal blockade. Gastroenterology 103: 1491–1497, 1992.[Web of Science][Medline]
  9. Coker RH, Krishna MG, Lacy DB, Allen EJ, and Wasserman DH. Sympathetic drive to liver and nonhepatic splanchnic tissue during heavy exercise. J Appl Physiol 82: 1244–1249, 1997.[Abstract/Free Full Text]
  10. Coker RH, Krishna MG, Zinker BA, Allen EJ, Lacy DB, and Wasserman DH. Sympathetic drive to liver and nonhepatic splanchnic tissue during prolonged exercise is increased in diabetes. Metabolism 46: 1327–1332, 1997.[CrossRef][Web of Science][Medline]
  11. Dobbins RL, Davis SN, Neal DW, Cobelli C, Jaspan J, and Cherrington AD. Compartmental modeling of glucagon kinetics in the conscious dog. Metabolism 44: 452–459, 1995.[CrossRef][Web of Science][Medline]
  12. Dunning BE, Moore MC, Ikeda T, Neal DW, Scott MF, and Cherrington AD. Portal glucose infusion exerts an incretin effect associated with changes in pancreatic neural activity in conscious dogs. Metabolism 51: 1324–1330, 2002.[CrossRef][Web of Science][Medline]
  13. Franz DN and Iggo A. Conduction failure in myelinated and non-myelinated axons at low temperatures. J Physiol 199: 319–345, 1968.[Abstract/Free Full Text]
  14. Hartmann H, Beckh K, and Jungermann K. Direct control of glycogen metabolism in the perfused rat liver by the sympathetic innervation. Eur J Biochem 123: 521–526, 1982.[Web of Science][Medline]
  15. Hendrick GK, Frizzell RT, Williams PE, and Cherrington AD. Effect of hyperglucagonemia on hepatic glycogenolysis and gluconeogenesis after a prolonged fast. Am J Physiol Endocrinol Metab 258: E841–E849, 1990.[Abstract/Free Full Text]
  16. Holste LC, Connolly CC, Moore MC, Neal DW, and Cherrington AD. Physiological changes in circulating glucagon alter hepatic glucose disposition during portal glucose delivery. Am J Physiol Endocrinol Metab 273: E488–E496, 1997.[Abstract/Free Full Text]
  17. Hsieh PS, Moore MC, Neal DW, Emshwiller M, and Cherrington AD. Rapid reversal of the effects of the portal signal under hyperinsulinemic conditions in the conscious dog. Am J Physiol Endocrinol Metab 276: E930–E937, 1999.[Abstract/Free Full Text]
  18. Hsieh PS, Moore MC, Neal DW, Venson P, and Cherrington AD. Hepatic glucose uptake rapidly decreases after elimination of the portal signal in conscious dogs. Am J Physiol Endocrinol Metab 275: E987–E992, 1998.[Abstract/Free Full Text]
  19. Ikeda T, Scott M, Emshwiller MG, Monohan MT, Allen E, Sindelar DK, and Dunning B. Hyperglycemia reduces sympathetic nervous signaling to the liver and gut in the dog (Abstract). Diabetes 46: 232A, 1997.
  20. Jackson PA, Pagliassotti MJ, Shiota M, Neal DW, Cardin S, and Cherrington AD. Effects of vagal blockade on the counterregulatory response to insulin-induced hypoglycemia in the dog. Am J Physiol Endocrinol Metab 273: E1178–E1188, 1997.[Abstract/Free Full Text]
  21. Leon DF, Shaver JA, and Leonard JJ. Reflex heart rate control in man. Am Heart J 80: 729–739, 1970.[CrossRef][Web of Science][Medline]
  22. Mari A. Estimation of the rate of appearance in the non-steady state with a two-compartment model. Am J Physiol Endocrinol Metab 263: E400–E415, 1992.[Abstract/Free Full Text]
  23. Myers SR, Biggers DW, Neal DW, and Cherrington AD. Intraportal glucose delivery enhances the effects of hepatic glucose load on net hepatic glucose uptake in vivo. J Clin Invest 88: 158–167, 1991.[Web of Science][Medline]
  24. Myers SR, McGuinness OP, Neal DW, and Cherrington AD. Intraportal glucose delivery alters the relationship between net hepatic glucose uptake and the insulin concentration. J Clin Invest 87: 930–939, 1991.[Web of Science][Medline]
  25. Nagase H, Inoue S, Tanaka K, Takamura Y, and Niijima A. Hepatic glucose-sensitive unit regulation of glucose-induced insulin secretion in rats. Physiol Behav 53: 139–143, 1993.[CrossRef][Medline]
  26. Narvanen S. Role of 5-hydroxytryptamine (serotonin) in oral glucose intolerance. Scand J Clin Lab Invest Suppl 167: 1–53, 1983.[Medline]
  27. Niijima A. Afferent impulse discharges from glucoreceptors in the liver of the guinea pig. Ann NY Acad Sci 157: 690–700, 1969.[Web of Science][Medline]
  28. Niijima A. Visceral afferents and metabolic function. Diabetologia Suppl 20: 325–330, 1981.
  29. Niijima A. Glucose-sensitive afferent nerve fibres in the hepatic branch of the vagus nerve in the guinea-pig. J Physiol 332: 315–323, 1982.[Abstract/Free Full Text]
  30. Niijima A. Electrophysiological study on nervous pathway from splanchnic nerve to vagus nerve in rat. Am J Physiol Regul Integr Comp Physiol 244: R888–R890, 1983.[Abstract/Free Full Text]
  31. Niijima A. Glucose-sensitive afferent nerve fibers in the liver and their role in food intake and blood glucose regulation. J Auton Nerv Syst 9: 207–220, 1983.[CrossRef][Web of Science][Medline]
  32. Niijima A. Reflex control of the autonomic nervous system activity from the glucose sensors in the liver in normal and midpontine-transected animals. J Auton Nerv Syst 10: 279–285, 1984.[CrossRef][Web of Science][Medline]
  33. Niijima A. Blood glucose levels modulate efferent activity in the vagal supply to the rat liver. J Physiol 364: 105–112, 1985.[Abstract/Free Full Text]
  34. Pagliassotti MJ, Holste LC, Moore MC, Neal DW, and Cherrington AD. Comparison of the time courses of insulin and the portal signal on hepatic glucose and glycogen metabolism in the dog. J Clin Invest 97: 81–91, 1996.[Web of Science][Medline]
  35. Pagliassotti MJ, Myers SR, Moore MC, Neal DW, and Cherrington AD. Magnitude of negative arterial-portal glucose gradient alters net hepatic glucose balance in conscious dogs. Diabetes 40: 1659–1668, 1991.[Abstract]
  36. Rothman DL, Magnusson I, Katz LD, Shulman RG, and Shulman GI. Quantitation of hepatic glycogenolysis and gluconeogenesis in fasting humans with 13C NMR. Science 254: 573–576, 1991.[Abstract/Free Full Text]
  37. Satake S, Moore MC, Igawa K, Converse M, Farmer B, Neal DW, and Cherrington AD. Direct and indirect effects of insulin on glucose uptake and storage by the liver. Diabetes 51: 1663–1671, 2002.[Abstract/Free Full Text]
  38. Scher AM and Young AC. Reflex control of heart rate in the unanesthetized dog. Am J Physiol 218: 780–789, 1970.[Free Full Text]
  39. Shimazu T. Glycogen synthetase activity in liver: regulation by the autonomic nerves. Science 156: 1256–1257, 1967.[Abstract/Free Full Text]
  40. Shimazu T. Neuronal regulation of hepatic glucose metabolism in mammals. Diabetes Metab Rev 3: 185–206, 1987.[Medline]
  41. Shimizu N, Oomura Y, Novin D, Grijalva CV, and Cooper PH. Functional correlations between lateral hypothalamic glucose-sensitive neurons and hepatic portal glucose-sensitive units in rat. Brain Res 265: 49–54, 1983.[CrossRef][Web of Science][Medline]
  42. Shiota M, Jackson P, Galassetti P, Scott M, Neal DW, and Cherrington AD. Combined intraportal infusion of acetylcholine and adrenergic blockers augments net hepatic glucose uptake. Am J Physiol Endocrinol Metab 278: E544–E552, 2000.[Abstract/Free Full Text]
  43. Stumpel F and Jungermann K. Sensing by intrahepatic muscarinic nerves of a portal-arterial glucose concentration gradient as a signal for insulin-dependent glucose uptake in the perfused rat liver. FEBS Lett 406: 119–122, 1997.[CrossRef][Web of Science][Medline]
  44. Stumpel F, Kucera T, Bazotte R, and Puschel GP. Loss of regulation by sympathetic hepatic nerves of liver metabolism and haemodynamics in chronically streptozotocin-diabetic rats. Diabetologia 39: 161–165, 1996.[CrossRef][Medline]
  45. Xue C, Aspelund G, Sritharan KC, Wang JP, Slezak LA, and Andersen DK. Isolated hepatic cholinergic denervation impairs glucose and glycogen metabolism. J Surg Res 90: 19–25, 2000.[CrossRef][Web of Science][Medline]
  46. Yamatani K, Ohnuma H, Niijima A, Igarashi M, Sugiyama K, Daimon M, Manaka H, Tominaga M, and Sasaki H. Impaired vagus nerve-mediated control of insulin secretion in Wistar fatty rats. Metabolism 47: 1167–1173, 1998.[CrossRef][Web of Science][Medline]
  47. Yamazaki M and Sakaguchi T. Pancreatic vagal functional distribution in the secretion of insulin evoked by portal infusion of D-glucose. Brain Res 484: 357–360, 1989.[CrossRef][Web of Science][Medline]




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