AJP - Regu Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Regul Integr Comp Physiol 290: R616-R624, 2006. First published October 27, 2005; doi:10.1152/ajpregu.00161.2005
0363-6119/06 $8.00
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
290/3/R616    most recent
00161.2005v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (5)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nakade, Y.
Right arrow Articles by Takahashi, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nakade, Y.
Right arrow Articles by Takahashi, T.

APPETITE, OBESITY, DIGESTION, AND METABOLISM

Restraint stress augments postprandial gastric contractions but impairs antropyloric coordination in conscious rats

Yukiomi Nakade, Daisuke Tsuchida, Hiroyuki Fukuda, Masahiro Iwa, Theodore N. Pappas, and Toku Takahashi

Department of Surgery, Duke University Medical Center, and Durham Veterans Affairs Medical Center, Durham, North Carolina

Submitted 4 March 2005 ; accepted in final form 20 October 2005


   ABSTRACT
TOP
 ABSTRACT
MATERIALS AND METHODS
 RESULTS
 DISCUSSION
 GRANTS
 REFERENCES
 
Central corticotropin-releasing factor (CRF) plays an important role in mediating restraint stress-induced delayed gastric emptying. However, it is unclear how restraint stress modulates gastric motility to delay gastric emptying. Inasmuch as solid gastric emptying is regulated via antropyloric coordination, we hypothesized that restraint stress impairs antropyloric coordination, resulting in delayed solid gastric emptying in conscious rats. Two strain gauge transducers were sutured onto the serosal surface of the antrum and pylorus, and postprandial gastric motility was monitored before, during, and after restraint stress. Antropyloric coordination, defined as a propagated single contraction from the antrum to the pylorus within 10 s, was followed by ≥20 s of quiescence. Restraint stress enhanced postprandial gastric motility in the antrum and pylorus to 140 ± 9% and 134 ± 9% of basal, respectively (n = 6). The number of episodes of antropyloric coordination before restraint stress, 2.4 ± 0.4/10 min, was significantly reduced to 0.6 ± 0.3/10 min by restraint stress. Intracisternal injection of the CRF type 2 receptor antagonist astressin 2B (60 µg) or guanethidine partially restored restraint stress-induced impairment of antropyloric coordination (1.6 ± 0.3/10 min, n = 6). The restraint stress-induced augmentation of antral and pyloric contractions was increased by astressin 2B and guanethidine but abolished by atropine, hexamethonium, and vagotomy. Restraint stress enhanced postprandial gastric motility via a vagal cholinergic pathway. Restraint stress-induced delay of solid gastric emptying is due to impairment of antropyloric coordination. Restraint stress-induced impairment of antropyloric coordination might be mediated via a central CRF pathway.

corticotropin-releasing factor; restraint stress

A GROWING BODY OF EVIDENCE suggests a role for stress in gastrointestinal (GI) physiological and pathophysiological functions, such as gastric emptying, gastric ulcerations, gastric secretion, and intestinal transit (1, 18, 19, 23, 28, 34, 41).

Several brain neuropeptides are known to act as stress-related neurotransmitters that modify GI motor functions (8, 29, 33, 37, 39). Corticotropin-releasing factor (CRF), one of the stress-related neuropeptides, is known to act in the brain to influence GI functions. Exogenous administration of CRF into the central nervous system (CNS) delays gastric emptying and inhibits acid secretion, whereas it stimulates colonic transit through the autonomic nervous system (5, 22, 35). Restraint stress augments CRF mRNA in the amygdala and paraventricular nucleus (PVN) (13, 16), resulting in alteration of GI motor activities. Restraint stress-induced delayed gastric emptying is abolished by central administration of CRF antagonist (23), suggesting that endogenous CRF has an important role in mediating stress-induced delay of gastric emptying. We recently demonstrated that restraint stress delays solid gastric emptying via a central CRF and peripheral sympathetic neuron in rats (30).

Although CRF and restraint stress attenuate gastric emptying (22), little is known about the postprandial gastric motility mediated by restraint stress. It remains unclear how restraint stress modifies gastric motility to delay gastric emptying.

Liquid meal emptying primarily reflects the activity of the fundus (27), whereas gastric emptying of a solid meal is regulated by coordination of the antrum, pylorus, and duodenum (3, 11, 15, 27).

The postprandial gastric motor pattern in response to a solid food involves a grinding and an emptying period in humans (3), dogs (40), and rats (14, 15). Immediately after a feeding, a grinding period, i.e., significant contraction, is observed in the antrum and pylorus. In contrast, the contractile pattern of the antrum is significantly changed from 40 min after a feeding in rats. The coordinated motility between the antrum and pylorus, which seems to be effective in propelling the gastric contents to the duodenum, is observed in this emptying period. Thus coordinated postprandial antropyloric contractions play an important role in the gastric emptying of solids in rats (14, 15).

We previously showed that hyperglycemia impairs antropyloric coordination and delays solid gastric emptying in conscious rats (15). We also demonstrated that injection of neuropeptide Y (NPY) into the CNS increased gastric motility but impaired antropyloric coordination, resulting in delayed solid gastric emptying in conscious rats (14). These results led us to speculate that restraint stress could impair antropyloric coordination and delay solid gastric emptying.

The present study was designed to examine the restraint stress-induced alteration of gastric motility from the viewpoint of postprandial antropyloric coordination. We assessed the mechanisms of restraint stress-mediated antropyloric coordination. In addition, we used a CRF type 2 receptor antagonist, astressin 2B, to examine the role of endogenous CRF in restraint stress-induced alteration of gastric motility.


   MATERIALS AND METHODS
TOP
 ABSTRACT
 MATERIALS AND METHODS
RESULTS
 DISCUSSION
 GRANTS
 REFERENCES
 
Animals

Male Sprague-Dawley rats (280–320 g body wt) were housed in group cages under conditions of controlled temperature (22–24°C), humidity, and illumination (12 h of light starting at 6 AM) for ≥7 days before the experiments and maintained on laboratory chow and water. After surgery, the rats were housed individually with access to a standard diet and tap water until the beginning of the experiments. All experiments were started at 9 AM. Protocols describing the use of rats were approved by the Institutional Animal Care and Use Committee of Durham Veterans Affairs Medical Center and in accordance with National Institutes of Health guidelines.

Substances and Treatments

A rat/human CRF and astressin 2B (both from Sigma, St. Louis, MO) were kept in powder form, CRF at –70°C and astressin 2B at room temperature. Peptides were dissolved in 0.9% saline (CRF) or double-distilled water (astressin 2B) immediately before use. Under light (3%) isoflurane anesthesia, rats were placed in a stereotaxic apparatus (model 900; David Kopf Instruments, Tujunga, CA). Peptides or vehicles were injected intracisternally (5 µl/rat) by puncture of the occipital membrane with a needle of a 10-µl microsyringe (Hamilton, Reno, NV). The presence of cerebrospinal fluid in the syringe on aspiration before injection verified the accuracy of needle placement into the cisterna magna, as previously described (30, 31).

Experimental Design

Recording of antral and pyloric motility in restraint stress. After 12 h of fasting, the rats were anesthetized with pentobarbital sodium (45 mg/kg ip). An abdominal incision was performed, and two strain gauge force transducers were sutured onto the serosal surface of the antrum and pylorus for recording of circular muscle contractions, as previously described (14, 15). The wires to the transducers were run under the skin to an opening made in the back of the neck using a rat protective system (Star Medical, Tokyo, Japan). After the transducers were sutured, the abdominal wall was closed.

At 1 wk after the operation, the rats were fasted for 12 h just before monitoring of gastric motility. The wires from the transducers were connected to the recording system (Power-Lab model 8SP; ADInstruments, Colorado Springs, CO). The rats were fed 3 g of rat chow, and gastric motor activities were recorded for 90 min before the restraint stress loading. The rats were lightly anesthetized (3% isoflurane) and placed on a wooden plate with their trunks wrapped in a confining harness consisting of a bandage fabricated from packaging tape. The animal was able to move its limbs and head but not its trunk (2, 23). Gastric motility was measured during restraint stress for 90 min. After 90 min of restraint stress loading, the rats were immediately released, and gastric motility was recorded for 90 min.

The area under the curve was calculated using a computer-assisted system (MacLab, ADInstruments) and expressed as a motility index. Each rat was subjected to the restraint stress loading at ≥4-day intervals.

Ascending contraction and descending relaxation play a crucial role in promoting effective peristalsis in the GI tract (9). When contractions occur simultaneously in adjacent parts of the GI tract, the content between those parts would not be expelled effectively. Thus the temporal relation of contraction between adjacent parts of the gut needs to be analyzed. As previously described (14, 15), antropyloric coordination was defined as a single contraction at the antrum that propagated aborally in the pylorus within 10 s and was followed by ≥20 s of quiescence. Individual contractions were defined as >2-g changes of >1-s duration. To investigate how restraint stress alters the motility of the antrum and pylorus, we evaluated the number of episodes of antropyloric coordination during restraint stress, as previously described (14).

Pharmacological and surgical approaches. To investigate whether restraint stress-induced alteration of gastric motility is mediated via a noradrenergic and cholinergic pathway, guanethidine (5.0 mg/kg ip) and atropine sulfate (50 µg/kg ip) were injected 30 min before the restraint stress loading. To evaluate the involvement of nicotinic receptors in the mediation of restraint stress, hexamethonium (20 mg/kg ip) was injected 30 min before the restraint stress loading. The dose of these antagonists was chosen on the basis of our previous studies (30, 38). Saline (0.3 ml)-injected rats served as controls.

To study the role of the vagus nerve in stress-induced alteration of gastric motility, rats underwent truncal vagotomy: the vagal trunks were cut around the abdominal esophagus at the time the transducers were sutured, as previously reported (32). Sham-operated rats served as controls. The feeding behavior was not affected by truncal vagotomy.

To examine the role of endogenous CRF in restraint stress-induced modification of gastric motility, the CRF type 2 receptor antagonist astressin 2B (60 µg/rat) or saline was injected intracisternally (ic) 10 min before the restraint stress loading under light (3%) isoflurane anesthesia. It has been shown that astressin 2B (60 µg ic) had a tendency to prevent the inhibitory effect of urocortin on fasting gastric motility and that astressin 2B (100 µg ic) had a statistically significant effect on the urocortin-induced inhibition of gastric motility in anesthetized rats (4). As shown in Fig. 8, CRF (0.5 µg ic) attenuated postprandial gastric motility in conscious rats. In our preliminary study, 60 µg of astressin 2B almost completely abolished CRF (0.5 µg ic)-induced inhibition of gastric motility.


Figure 8
View larger version (31K):
[in this window]
[in a new window]
  		Fig. 8. Effects of intracisternal injection of saline (A) and corticotropin- releasing factor (CRF) (0.1–1 µg; B–E) on antral motility. Postprandial antral motility was monitored before and after intracisternal injection of CRF and saline. CRF (0.1 µg ic) did not modify postprandial antral motility. At 0.25 µg, CRF dose dependently attenuated postprandial antral motility. Inhibitory effects of CRF were sustained for up to 30 min.

 
To investigate whether the sympathetic pathway is involved in endogenous CRF-induced mediation of gastric motility, we injected astressin 2B (60 µg ic) into guanethidine-treated rats.

Recording of antral and pyloric motility in response to intracisternal CRF. After 12 h of fasting, the rats were fed 3 g of rat chow, and gastric motor activities were recorded for 90 min before administration of CRF (0.1–1.0 µg/rat). CRF or saline was administered by intracisternal injection while the animals were under light (3%) isoflurane anesthesia. After the peptide injection, gastric motility was monitored for 180 min, and the CRF-induced alteration of gastric motility was evaluated. The number of episodes of antropyloric coordination was also counted before and after CRF injection for 30 min. To investigate the involvement of sympathetic nerves on CRF-induced alteration of gastric motility, guanethidine (5.0 mg/kg ip) was injected 30 min before CRF injection.

Statistical Analysis

Values are means ± SE. Data from control animals and those subjected to restraint stress were compared by paired Student's t-test. Data from sham-operated control rats and rats subjected to truncal vagotomy were compared by Student's t-test. A multiple-group comparison was performed by ANOVA followed by Fisher's protected least significant difference method. P < 0.05 was considered statistically significant.


   RESULTS
TOP
 ABSTRACT
 MATERIALS AND METHODS
 RESULTS
DISCUSSION
 GRANTS
 REFERENCES
 
Effect of Restraint Stress on Postprandial Antropyloric Coordination

At 90 min after feeding, the contraction maxima of the antrum occurred most often 3–6 s before the contraction maxima of the pylorus (Fig. 1), as previously reported (14, 15). The number of episodes of antropyloric coordination was 2.4 ± 0.4/10 min before the restraint stress (Table 1).


Figure 1
View larger version (25K):
[in this window]
[in a new window]
  		Fig. 1. Effect of restraint stress on postprandial antral and pyloric motility. In prerestraint period, a time lag was most frequently observed between each contraction of antrum and pylorus. Vertical lines, episode of antropyloric coordination. Contraction maxima of antrum occurred most often 3–6 s before contraction maxima of pylorus. Restraint stress enhanced antral and pyloric motility. During restraint stress loading, contraction maxima of antrum and pylorus were observed simultaneously. Arrows, simultaneous contraction of antrum and pylorus. Peak amplitude and frequency of gastric antrum were increased by restraint stress. At termination of stress loading, contractile pattern of antrum and pylorus returned to basal.

 

View this table:
[in this window]
[in a new window]
  		Table 1. Episodes on antropyloric coordination mediated by restraint stress or central CRF

 
Restraint stress enhanced antral and pyloric motility compared with the prerestraint state. Restraint stress increased the peak amplitude of the antrum and pylorus to 137 ± 11% and 125 ± 12% of basal (n = 6), respectively. Restraint stress increased the frequency of antral and pyloric contractions from 2.2 ± 0.5 to 3.8 ± 0.6 and from 2.8 ± 0.5 to 4.1 ± 0.7/min, respectively. Thus the motility index of the antrum and pylorus was also increased from 23,724 ± 2,467 to 33,450 ± 2,763 and from 10,524 ± 927 to 14,102 ± 1,242 g/s for 30 min, respectively (Fig. 2). During the restraint stress loading, the time lag between the antral and pyloric contractions was not observed. The contraction maxima of the antrum and pylorus occurred simultaneously (Fig. 1). The number of episodes of antropyloric coordination was significantly reduced to 0.6 ± 0.3/10 min during the restraint stress (Table 1).


Figure 2
View larger version (17K):
[in this window]
[in a new window]
  		Fig. 2. Effects of saline, guanethidine, atropine, and hexamethonium on restraint stress-induced augmentation of antral (A) and pyloric (B) motility in conscious rats. Motility indexes of antrum and pylorus were augmented by restraint stress. Motility index of antrum induced by restraint stress was further enhanced by guanethidine. Although motility index of pylorus induced by restraint stress was augmented, there was no significant difference compared with saline-treated group (n = 4–6). **P < 0.01 compared with control. #P < 0.05 compared with saline. Atropine and hexamethonium attenuated motility index. Restraint stress did not induce any change of motility mediated by atropine and hexamethonium. NS, not significant.

 
Restraint stress-induced augmentation of the antral and pyloric contractions returned to the basal levels within 3–5 min after termination of stress loading (Fig. 1). The time lag between the antral and pyloric contractions was also observed after termination of restraint stress loading (Fig. 1).

Effect of Guanethidine, Atropine, Hexamethonium, and Truncal Vagotomy on Restraint Stress-Induced Alteration of Antral and Pyloric Motility

Guanethidine enhanced restraint stress-induced augmentation of antral and pyloric contractions (Fig. 3B). During the restraint stress, guanethidine increased the peak amplitude of the antrum and pylorus to 145 ± 21% and 146 ± 10% of basal (n = 6), respectively. During the restraint stress, guanethidine increased the frequency of antral and pyloric contractions from 2.6 ± 0.5 to 5.0 ± 0.2 and from 3.3 ± 0.8 to 5.1 ± 0.1/min, respectively. The motility index of the antrum was also increased from 25,500 ± 2,467 to 37,211 ± 2,342 g/s for 30 min (Fig. 2A) during stress loading. The motility index of the pylorus tended to be enhanced by guanethidine, although the difference was not statistically significant (Fig. 2B). The number of episodes of antropyloric coordination was partially restored to 1.5 ± 0.4/10 min by guanethidine during stress loading (Table 1).


Figure 3
View larger version (29K):
[in this window]
[in a new window]
  		Fig. 3. Effects of saline (A), guanethidine (Gua, B), atropine (Atr, C), and hexamethonium (Hex, D) on restraint stress-induced augmentation of antral and pyloric motility in conscious rats. Guanethidine enhanced restraint stress-induced augmentation of antral and pyloric motility. Atropine and hexamethonium attenuated antral and pyloric motility induced by restraint stress.

 
Pretreatment with atropine and hexamethonium reduced postprandial antral and pyloric contractions and abolished restraint stress-induced augmentation of the antral and pyloric contractions (Fig. 3, C and D). During the restraint stress loading, no significant changes were observed in the antrum and pylorus in atropine- and hexamethonium-treated rats (Fig. 2, A and B).

Postprandial antral and pyloric contractions were attenuated in vagotomized compared with sham-operated rats (Fig. 4, B and C). Restraint stress did not affect postprandial antral and pyloric contractions in vagotomized rats (Fig. 4).


Figure 4
View larger version (21K):
[in this window]
[in a new window]
  		Fig. 4. A: effects of truncal vagotomy on restraint stress-induced augmentation of antral and pyloric motility in conscious rats. Truncal vagotomy reduced postprandial antral (B) and pyloric (C) motility indexes. Restraint stress did not augment antral and pyloric motility in vagotomized rats. Values are means ± SE (n = 4–5). *P < 0.05 compared with control. **P < 0.01 compared with respective groups.

 
Effect of Intracisternal Injection of Astressin 2B on Restraint Stress-Induced Augmentation of Antral and Pyloric Motility

Intracisternal astressin 2B (60 µg) did not affect postprandial antral and pyloric contractions (Fig. 5, A and B). Intracisternal astressin 2B significantly enhanced restraint stress-induced augmentation of antral and pyloric contractions (Fig. 6) and increased the motility index of antral and pyloric contractions stimulated by restraint stress (Fig. 5, A and B).


Figure 5
View larger version (18K):
[in this window]
[in a new window]
  		Fig. 5. Effect of intracisternal (ic) injection of astressin 2B on restraint stress-induced augmentation of antral (A) and pyloric (B) motility. Restraint stress-induced augmentation of antral and pyloric motility was further enhanced by astressin 2B (60 µg ic). Guanethidine also increased restraint stress-induced augmentation of antral motility. Intracisternal astressin 2B did not further increase antral and pyloric motility in guanethidine-treated rats. Guanethidine also tended to increase restraint stress-induced augmentation of pyloric motility, although this was not statistically significant (NS). Values are means ± SE (n = 4–6). *P < 0.05; **P < 0.01 compared with saline. #P < 0.05 compared with saline/restraint stress.

 

Figure 6
View larger version (28K):
[in this window]
[in a new window]
  		Fig. 6. Effect of intracisternal astressin 2B on restraint stress-induced augmentation of gastric motility. Astressin 2B (60 µg ic) further enhanced antral and pyloric motility mediated by restraint stress. Before stress loading, time lag was most frequently detected between each contraction of antrum and pylorus. Vertical line, antropyloric coordination. Arrow, simultaneous contraction of antrum and pylorus. Even during stress loading, time lag was frequently observed and antropyloric coordination was detected. After restraint stress loading, contractile pattern of both motilities returned to prerestraint pattern.

 
During restraint stress loading, there was an obvious time lag between the antral and pyloric contractions in astressin 2B-treated rats (Fig. 6). The number of episodes of antropyloric coordination was partially restored to 1.6 ± 0.3/10 min during the restraint stress loading (Table 1).

In guanethidine-treated rats, intracisternal astressin 2B did not further increase the motor activities stimulated by restraint stress (Fig. 5, A and B). The time lag between the antral and pyloric contractions was also observed in animals treated with astressin 2B + guanethidine (Fig. 7). The number of episodes of antropyloric coordination was also restored to 1.4 ± 0.5/10 min during the restraint stress loading (Table 1).


Figure 7
View larger version (29K):
[in this window]
[in a new window]
  		Fig. 7. Effect of guanethidine on restraint stress-induced augmentation of gastric motility mediated by intracisternal astressin 2B. Astressin 2B (60 µg ic) did not further enhance restraint stress-induced antral and pyloric motility. During stress loading, time lag was observed between antrum and pylorus. Arrow, simultaneous contraction of antrum and pylorus. Vertical lines, episodes of antropyloric coordination, which were seen even during stress loading.

 
Effect of Intracisternal Injection of CRF on Postprandial Antral and Pyloric Contractions

Intracisternal injection of saline or CRF (0.1 µg) did not affect postprandial antral contractions (Figs. 8, A and B, and 9). In contrast, intracisternal injection of CRF (0.25, 0.5, and 1.0 µg) inhibited postprandial antral contractions in a dose-dependent manner (Figs. 8, C–E, and 9). The inhibitory effects were observed for 30 min after CRF (1.0 µg) injection (Fig. 8E). The inhibitory effect of CRF on the pyloric contractions was also observed (data not shown).


Figure 9
View larger version (15K):
[in this window]
[in a new window]
  		Fig. 9. Motility indexes of antrum and pylorus were dose dependently attenuated by CRF (0.25–1.0 µg ic) in postprandial state. Values are means ± SE (n = 4–6). *P < 0.05; **P < 0.01 compared with saline control.

 
Intracisternal injection of CRF (0.5 µg) increased the number of simultaneous contractions of the antrum and pylorus (Fig. 10) and significantly attenuated the number of episodes of antropyloric coordination for up to 30 min after the peptide injection (Table 1).


Figure 10
View larger version (21K):
[in this window]
[in a new window]
  		Fig. 10. Effects of intracisternal injection of 0.5 µg of CRF on antropyloric coordination. Intracisternal injection of CRF attenuated gastric contractions in antrum and pylorus. Before CRF injection, time lag was most frequently observed between each contraction of antrum and pylorus. Contraction maxima of antrum and pylorus were observed almost simultaneously for up to 30 min after CRF injection. Arrows, simultaneous contraction of antrum and pylorus. Vertical lines, episodes of antropyloric coordination.

 
Pretreatment with guanethidine almost completely abolished the inhibitory effects of CRF on postprandial antral and pyloric contractions (Fig. 11).


Figure 11
View larger version (26K):
[in this window]
[in a new window]
  		Fig. 11. Effects of saline (A) and guanethidine (B) on intracisternal CRF-induced inhibition of antral (C) and pyloric (D) contraction. Guanethidine abolished CRF-induced inhibition of gastric motility. Values are means ± SE (n = 4–5). **P < 0.01 compared saline.

 

   DISCUSSION
TOP
 ABSTRACT
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
GRANTS
 REFERENCES
 
A number of investigators reported that restraint stress delays gastric emptying of a liquid (4, 23, 26) and solid food (30). However, little is known about whether restraint stress modifies gastric motility. Although it has been reported that restraint stress does not affect gastric motility in a fasting state (42), it remains unclear whether restraint stress alters postprandial gastric motility.

Our present study demonstrated that restraint stress significantly augmented gastric contractions of the antrum and pylorus in response to solid food ingestion. We also showed that the augmented gastric motility was restored to basal levels immediately after termination of the restraint stress loading. Because atropine, hexamethonium, and vagotomy blocked restraint stress-induced augmentation of gastric motility, the vagal-cholinergic pathway might be involved in this event.

Previous reports indicate that the CNS produces several neuropeptides in response to restraint stress (16, 20). Cold-restraint stress increases thyrotropin-releasing hormone (TRH) in the brain stem, which stimulates gastric motility and liquid emptying (17, 36). It remains unknown whether endogenous TRH is involved in mediating restraint stress-induced augmentation of postprandial gastric contractions. Some other unknown factors might be involved in the mechanism of restraint stress-induced augmentation of gastric motility.

It is generally accepted that solid gastric emptying is regulated by coordination of the antrum, pylorus, and duodenum (11, 27). In particular, coordination between the antrum and pylorus is an important factor in the emptying of solid foods (3, 6, 14, 21, 27).

In pre- and postrestraint stress, a time lag was observed between the antral and pyloric contractions; most often, the contraction maxima of the antrum occurred 3–6 s before the contraction maxima of the pylorus.

During restraint stress loading, the time lag between the antral and pyloric contractions was not observed frequently and the contraction maxima of the antrum and pylorus occurred simultaneously. The number of episodes of antropyloric coordination was significantly reduced during the restraint stress. Simultaneous contractions of the antrum and pylorus are considered to impair the effective emptying process and delay gastric emptying of a solid food.

It remains unclear whether the augmented gastric contractions are well correlated with the acceleration of gastric emptying. Pentagastrin significantly augments gastric antral contractions, but it does not accelerate gastric emptying (12, 25). Although restraint stress-induced augmentation of gastric contractions was further increased by guanethidine, gastric emptying was not fully restored (30). Thus augmented gastric motility does not necessarily improve delayed gastric emptying. The coordinated motor pattern between the antrum and pylorus seems to be more important than augmented gastric contractions in mediating gastric emptying of solid food.

CRF is known to act in the brain as a neurotransmitter to influence GI function through the autonomic nervous system (5, 7, 22, 30). The present study revealed that intracisternal injection of CRF dose dependently attenuated postprandial gastric motility of the antrum and pylorus for up to 30 min. Furthermore, we demonstrated that intracisternal injection of CRF (0.5 µg) produced simultaneous contractions, resulting in impairment of antropyloric coordination. These results indicated that administration of CRF into the CNS not only attenuates gastric motility but also impairs antropyloric coordination. It is conceivable that endogenous CRF may be involved in the impairment of antropyloric coordination in stress loading.

The pathway through which central CRF attenuates gastric motility remains to be elucidated. A previous report demonstrated that microinjection of CRF at the dorsal vagal complex attenuates bethanechol-stimulated gastric contractions in anesthetized rats (24). The inhibitory effect of CRF is mediated via activation of inhibitory nonadrenergic noncholinergic vagal input to the stomach (24). In contrast, our present study demonstrated that guanethidine abolished inhibition of postprandial gastric motility induced by intracisternal injection of CRF in conscious rats, suggesting involvement of the sympathetic pathway.

We also showed that intracisternal injection of the CRF type 2 receptor antagonist astressin 2B (60 µg) enhanced restraint stress-induced augmentation of gastric motility. The pathophysiological significance of the inhibitory effect of central CRF on postprandial gastric motility remains unclear. It has been reported that hypothalamic microinfusions of CRF increase bicarbonate content, decrease gastric acid content, and protect against cold restraint-induced gastric mucosal damage (10). It is conceivable that central CRF may act against stress-induced augmentation of gastric motility. This may provide new information about the role of endogenous CRF in stress-induced alteration of postprandial gastric motility.

Restraint stress-induced impairment of antropyloric coordination was partially restored by treatment with guanethidine. Intracisternal injection of astressin 2B did not produce any further changes in stress-induced impaired coordination in guanethidine-treated rats. These results suggest that the inhibitory effect of endogenous CRF on gastric motility is mediated via sympathetic pathways.

Although intracisternal injection of astressin 2B did not fully restore impaired antropyloric coordination induced by restraint stress (Table 1), our previous study showed that restraint stress-induced delay of gastric emptying was completely restored by astressin 2B (30). The reason for this difference remains to be elucidated. One possibility may be the difference in the stress models. In our previous study, partial body-tubing stress was applied (30); in the present study, partial body-wrapping stress was used. In the wrapping stress model, stress-induced delay of solid gastric emptying might not be completely inhibited by intracisternal injection of astressin 2B.

We cannot exclude the possibility that other stress-related neuronal factors, in addition to CRF, might be involved in the impaired antropyloric coordination induced by partial body-wrapping stress. Several central coding neuropeptides, such as NPY or TRH, seem to be involved in the mechanism of stress-induced alteration of gastric motor functions (14, 17, 20). We previously showed that centrally administered NPY impairs antropyloric coordination (14). It is of interest to examine whether stress-induced impairment of antropyloric coordination is mediated by these neuropeptides.

In summary, restraint stress augmented postprandial gastric motility but impaired antropyloric coordination in conscious rats. The augmented postprandial gastric motility is mediated via vagal cholinergic pathways. Central CRF in response to restraint stress may play an important role in impairment of postprandial antropyloric coordination. Delayed gastric emptying by restraint stress might be due to impairment of antropyloric coordination. These results provide new evidence that restraint stress modifies gastric motor function.


   GRANTS
TOP
 ABSTRACT
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
 GRANTS
REFERENCES
 
This study was supported in part by National Institute of Diabetes and Digestive and Kidney Diseases Grant RO1-DK-55808 (to T. Takahashi).


   FOOTNOTES
 

Address for reprint requests and other correspondence: T. Takahashi, Surgical Service 112, VA Medical Center, 508 Fulton St., Durham, NC 27705 (e-mail: ttakahas{at}duke.edu)

The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.


   REFERENCES
TOP
 ABSTRACT
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
 GRANTS
 REFERENCES
 

  1. Barone FC, Deegan JF, Price WJ, Fowler PJ, Fondacaro JD, and Ormsbee HS III. Cold-restraint stress increases rat fecal pellet output and colonic transit. Am J Physiol Gastrointest Liver Physiol 258: G329–G337, 1990.[Abstract/Free Full Text]
  2. Bradesi S, Eutamene H, Fioramonti J, and Bueno L. Acute restraint stress activates functional NK1 receptor in the colon of female rats: involvement of steroids. Gut 50: 349–354, 2002.[Abstract/Free Full Text]
  3. Brown BP, Schulze-Delrieu K, Schrier JE, and Abu-Yousef MM. The configuration of the human gastroduodenal junction in the separate emptying of liquids and solids. Gastroenterology 105: 433–440, 1993.[ISI][Medline]
  4. Chen CY, Million M, Adelson DW, Martinez V, Rivier J, and Tache Y. Intracisternal urocortin inhibits vagally stimulated gastric motility in rats: role of CRF2. Br J Pharmacol 136: 237–247, 2002.[CrossRef][ISI]
  5. Druge G, Raedler A, Greten H, and Lenz HJ. Pathways mediating CRF-induced inhibition of gastric acid secretion in rats. Am J Physiol Gastrointest Liver Physiol 256: G214–G219, 1989.[Abstract/Free Full Text]
  6. Ehrlein HJ and Hiesinger E. Computer analysis of mechanical activity of gastroduodenal junction in unanaesthetized dogs. Q J Exp Physiol 67: 17–29, 1982.[Abstract/Free Full Text]
  7. Garrick T, Buack S, Veiseh A, and Tache Y. Thyrotropin-releasing hormone (TRH) acts centrally to stimulate gastric contractility in rats. Life Sci 40: 649–657, 1987.[CrossRef][ISI][Medline]
  8. Geoghegan JG, Lawson DC, Cheng CA, Opara E, Taylor IL, and Pappas TN. Intracerebroventricular neuropeptide Y increases gastric and pancreatic secretion in the dog. Gastroenterology 105: 1069–1077, 1993.[ISI][Medline]
  9. Grider JR and Makhlouf GM. Colonic peristaltic reflex: identification of vasoactive intestinal peptide as mediator of descending relaxation. Am J Physiol Gastrointest Liver Physiol 251: G40–G45, 1986.[Abstract/Free Full Text]
  10. Gunion MW, Kauffman GL Jr, and Tache Y. Intrahypothalamic corticotropin-releasing factor elevates gastric bicarbonate and inhibits stress ulcers in rats. Am J Physiol Gastrointest Liver Physiol 258: G152–G157, 1990.[Abstract/Free Full Text]
  11. Haba T and Sarna SK. Regulation of gastroduodenal emptying of solids by gastropyloroduodenal contractions. Am J Physiol Gastrointest Liver Physiol 264: G261–G271, 1993.[Abstract/Free Full Text]
  12. Hamilton SG, Sheiner HJ, and Quinlan MF. Continuous monitoring of the effect of pentagastrin on gastric emptying of solid food in man. Gut 17: 273–279, 1976.[Abstract/Free Full Text]
  13. Imaki T, Naruse M, Harada S, Chikada N, Nakajima K, Yoshimoto T, and Demura H. Stress-induced changes of gene expression in the paraventricular nucleus are enhanced in spontaneously hypertensive rats. J Neuroendocrinol 10: 635–643, 1998.[CrossRef][ISI][Medline]
  14. Ishiguchi T, Amano T, Matsubayashi H, Tada H, Fujita M, and Takahashi T. Centrally administered neuropeptide Y delays gastric emptying via Y2 receptors in rats. Am J Physiol Regul Integr Comp Physiol 281: R1522–R1530, 2001.[Abstract/Free Full Text]
  15. Ishiguchi T, Tada H, Nakagawa K, Yamamura T, and Takahashi T. Hyperglycemia impairs antro-pyloric coordination in conscious rats. Auton Neurosci 95: 112–120, 2002.[CrossRef][ISI][Medline]
  16. Kalin NH, Takahashi LK, and Chen FL. Restraint stress increases corticotropin-releasing hormone mRNA content in the amygdala and paraventricular nucleus. Brain Res 656: 182–186, 1994.[CrossRef][ISI][Medline]
  17. Kaneko H, Tache Y, and Kusugami K. Importance of medullary thyrotropin-releasing hormone in brain-gut circuits regulating gastric integrity: preclinical studies. J Gastroenterol 37 Suppl 14: 128–132, 2002.[Medline]
  18. Koo MW, Cho CH, and Ogle CW. Effects of cold-restraint stress on gastric ulceration and motility in rats. Pharmacol Biochem Behav 25: 775–779, 1986.[CrossRef][ISI][Medline]
  19. Koo MW, Ogle CW, and Cho CH. The effect of cold-restraint stress on gastric emptying in rats. Pharmacol Biochem Behav 23: 969–972, 1985.[CrossRef][Medline]
  20. Krukoff TL, MacTavish D, and Jhamandas JH. Effects of restraint stress and spontaneous hypertension on neuropeptide Y neurones in the brainstem and arcuate nucleus. J Neuroendocrinol 11: 715–723, 1999.[Medline]
  21. Kumar D, Ritman EL, and Malagelada JR. Three-dimensional imaging of the stomach: role of pylorus in the emptying of liquids. Am J Physiol Gastrointest Liver Physiol 253: G79–G85, 1987.[Abstract/Free Full Text]
  22. Lenz HJ, Burlage M, Raedler A, and Greten H. Central nervous system effects of corticotropin-releasing factor on gastrointestinal transit in the rat. Gastroenterology 94: 598–602, 1988.[ISI][Medline]
  23. Lenz HJ, Raedler A, Greten H, Vale WW, and Rivier JE. Stress-induced gastrointestinal secretory and motor responses in rats are mediated by endogenous corticotropin-releasing factor. Gastroenterology 95: 1510–1517, 1988.[ISI][Medline]
  24. Lewis MW, Hermann GE, Rogers RC, and Travagli RA. In vitro and in vivo analysis of the effects of corticotropin-releasing factor on rat dorsal vagal complex. J Physiol 543: 135–146, 2002.[Abstract/Free Full Text]
  25. Marzio L and Lanfranchi GA. Motor coordination of the gastro-duodenal junction in the dog [in Italian]. Boll Soc Ital Biol Sper 56: 126–132, 1980.[Medline]
  26. Million M, Maillot C, Saunders P, Rivier J, Vale W, and Tache Y. Human urocortin II, a new CRF-related peptide, displays selective CRF2-mediated action on gastric transit in rats. Am J Physiol Gastrointest Liver Physiol 282: G34–G40, 2002.[Abstract/Free Full Text]
  27. Minami H and McCallum RW. The physiology and pathophysiology of gastric emptying in humans. Gastroenterology 86: 1592–1610, 1984.[ISI][Medline]
  28. Monnikes H, Schmidt BG, Raybould HE, and Tache Y. CRF in the paraventricular nucleus mediates gastric and colonic motor response to restraint stress. Am J Physiol Gastrointest Liver Physiol 262: G137–G143, 1992.[Abstract/Free Full Text]
  29. Monnikes H, Schmidt BG, and Tache Y. Psychological stress-induced accelerated colonic transit in rats involves hypothalamic corticotropin-releasing factor. Gastroenterology 104: 716–723, 1993.[ISI][Medline]
  30. Nakade Y, Tsuchida D, Fukuda H, Iwa M, Pappas TN, and Takahashi T. Restraint stress delays solid gastric emptying via a central CRF and peripheral sympathetic neuron in rats. Am J Physiol Regul Integr Comp Physiol 288: R427–R432, 2005.[Abstract/Free Full Text]
  31. Nakade Y, Yoneda M, Nakamura K, Makino I, and Terano A. Involvement of endogenous CRF in carbon tetrachloride-induced acute liver injury in rats. Am J Physiol Regul Integr Comp Physiol 282: R1782–R1788, 2002.[Abstract/Free Full Text]
  32. Nakao K, Takahashi T, Utsunomiya J, and Owyang C. Extrinsic neural control of nitric oxide synthase expression in the myenteric plexus of rat jejunum. J Physiol 507: 549–560, 1998.[Abstract/Free Full Text]
  33. Tache Y, Garrick T, and Raybould H. Central nervous system action of peptides to influence gastrointestinal motor function. Gastroenterology 98: 517–528, 1990.[ISI][Medline]
  34. Tache Y, Kolve E, Maeda HM, and Kauffman GJ. Central nervous system action of calcitonin to alter experimental gastric ulcers in rats. Gastroenterology 94: 145–150, 1988.[Medline]
  35. Tache Y, Maeda-Hagiwara M, and Turkelson CM. Central nervous system action of corticotropin-releasing factor to inhibit gastric emptying in rats. Am J Physiol Gastrointest Liver Physiol 253: G241–G245, 1987.[Abstract/Free Full Text]
  36. Tache Y, Maeda-Hagiwara M, Goto Y, and Garrick T. Central nervous system action of TRH to stimulate gastric function and ulceration. Peptides 9 Suppl 1: 9–13, 1988.[Medline]
  37. Tache Y, Martinez V, Million M, and Wang L. Stress and the gastrointestinal tract. III. Stress-related alterations of gut motor function: role of brain corticotropin-releasing factor receptors. Am J Physiol Gastrointest Liver Physiol 280: G173–G177, 2001.[Abstract/Free Full Text]
  38. Tatewaki M, Harris M, Uemura K, Ueno T, Hoshino E, Shiotani A, Pappas TN, and Takahashi T. Dual effects of acupuncture on gastric motility in conscious rats. Am J Physiol Regul Integr Comp Physiol 285: R862–R872, 2003.[Abstract/Free Full Text]
  39. Thiefin G, Tache Y, Leung FW, and Guth PH. Central nervous system action of thyrotropin-releasing hormone to increase gastric mucosal blood flow in the rat. Gastroenterology 97: 405–411, 1989.[ISI][Medline]
  40. Ueno T, Uemura K, Harris MB, Pappas TN, and Takahashi T. Role of vagus nerve in postprandial antropyloric coordination in conscious dogs. Am J Physiol Gastrointest Liver Physiol 288: G487–G495, 2005.[Abstract/Free Full Text]
  41. Williams CL, Villar RG, Peterson JM, and Burks TF. Stress-induced changes in intestinal transit in the rat: a model for irritable bowel syndrome. Gastroenterology 94: 611–621, 1988.[ISI][Medline]
  42. Yano S, Akahane M, and Harada M. Role of gastric motility in development of stress-induced gastric lesions of rats. Jpn J Pharmacol 28: 607–615, 1978.[Medline]



This article has been cited by other articles:


Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
H. Ariga, K. Imai, C. Chen, C. Mantyh, T. N. Pappas, and T. Takahashi
Does ghrelin explain accelerated gastric emptying in the early stages of diabetes mellitus?
Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2008; 294(6): R1807 - R1812.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
H. Ariga, K. Imai, C. Chen, C. Mantyh, T. N. Pappas, and T. Takahashi
Fixed feeding potentiates interdigestive gastric motor activity in rats: importance of eating habits for maintaining interdigestive MMC
Am J Physiol Gastrointest Liver Physiol, March 1, 2008; 294(3): G655 - G659.
[Abstract] [Full Text] [PDF]

This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
290/3/R616    most recent
00161.2005v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (5)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nakade, Y.
Right arrow Articles by Takahashi, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nakade, Y.
Right arrow Articles by Takahashi, T.

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2006 by the American Physiological Society.