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LETTER TO THE EDITOR

ENDOCRINE PHYSIOLOGY AND METABOLISM

Response to the letter to the editor: "HIF-1 protein rather than mRNA as a marker of hypoxia in adipose tissue in obesity," by Trayhurn et al.

However, a previous study has demonstrated an increase of HIF1- protein levels in adipose tissue in obese subjects (2). Trayhurn et al. (5) strongly support the interest in studying HIF-1 protein rather than mRNA. We agree that in general, and when possible, the measure of the amount of the active form of the protein should be determined. Nevertheless, our samples were obtained during gastric bypass intervention under general anesthesia, while subcutaneous biopsies, as described by Cancello et al. (2), were performed after local anesthesia, which may make difficult any comparison. If anesthesia influences the HIF-1 mRNA level, it may contribute to conflicting observations. It is noteworthy that many abnormalities other than tissue hypoxia have been implicated in obesity, each of them influenced by the genetic background (1, 3), and our objective was not to evaluate the exact contribution of HIF-1 in comparison to others.

FOOTNOTES

Address for reprint requests and other correspondence: O. Poulain-Godefroy, Centre National de la Recherche Scientifique 8090-Institute of Biology, Pasteur Institute, 1 rue Calmette, 59019 Lille cedex, BP447, France (e-mail: odile.poulain{at}good.ibl.fr)

REFERENCES

1. Bell CG, Walley AJ, Froguel P. The genetics of human obesity. Nat Rev Genet 6: 221–234, 2005.[Web of Science][Medline]
2. Cancello R, Henegar C, Viguerie N, Taleb S, Poitou C, Rouault C, Coupaye M, Pelloux V, Hugol D, Bouillot JL, Bouloumie A, Barbatelli G, Cinti S, Svensson PA, Barsh GS, Zucker JD, Basdevant A, Langin D, Clement K. Reduction of macrophage infiltration and chemoattractant gene expression changes in white adipose tissue of morbidly obese subjects after surgery-induced weight loss. Diabetes 54: 2277–2286, 2005.[Abstract/Free Full Text]
3. Hofbauer KG. Molecular pathways to obesity. Int J Obes Relat Metab Disord 26, Suppl 2: S18–S27, 2002.
4. Poulain-Godefroy O, Lecoeur C, Pattou F, Fruhbeck G, Froguel P. Inflammation is associated with a decrease of lipogenic factors in omental fat in women. Am J Physiol Regul Integr Comp Physiol 295: R1–R7, 2008.[Abstract/Free Full Text]
5. Trayhurn P, Wang B, Wood IS. HIF-1 protein rather than mRNA as a marker of hypoxia in adipose tissue in obesity: focus on "Inflammation is associated with a decrease of lipogenic factors in omental fat in women." doi:10.1152/ajpregu.90633.2008.[Free Full Text]
6. Wang B, Wood I, Trayhurn P. Dysregulation of the expression and secretion of inflammation-related adipokines by hypoxia in human adipocytes. Pflügers Arch Eur J Physiol 455: 479–492, 2007.[CrossRef][Web of Science][Medline]

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