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1 Institute of Exercise and Sports Sciences, Copenhagen Muscle Research Center, University of Copenhagen, Copenhagen, Denmark
2 Sports Medicine Research Unit, Copenhagen University Hospital, Copenhagen, Denmark
* To whom correspondence should be addressed. E-mail: jbangsbo{at}aki.ku.dk.
Interstitial K+ ([K+]i) was measured in human skeletal muscle by microdialysis during exhaustive leg exercise, with (AL) and without (L) previous intense arm exercise. In addition, the reproducibility of the [K+]i determinations was examined. Possible microdialysis induced rupture of the sarcolemma was assessed by measurement of carnosine in the dialysate, since carnosine is only expected to be found intracellularly. Changes in [K+]i could be reproduced when exhaustive leg exercise was performed on two different days with a between day difference of around 0.5 mM at rest and 1.5 mM at exhaustion. The time to exhaustion was shorter in AL than in L (2.7±0.3 vs. 4.0±0.3 min; P<0.05). Furthermore, [K+]i was higher from 0-1.5 min of the intense leg exercise period in AL compared to L (9.2±0.7 vs. 6.4±0.9 mM; P<0.001) and at exhaustion (11.9±0.5 vs. 10.3±0.6 mM; P<0.05). The dialysate content of carnosine was elevated by exercise, but low intensity exercise resulted in higher dialysate carnosine concentrations than subsequent intense exercise. Furthermore, no relationship was found between carnosine concentrations and [K+]i. Thus, the present data suggest that microdialysis can be used to determine muscle [K+]i kinetics during intense exercise, when low intensity exercise is performed prior to the intense exercise. The high [K+]i levels reached at exhaustion can be expected to cause fatigue, which is supported by the finding that a faster accumulation of interstitial K+, induced by prior arm exercise, was associated with a reduced time to fatigue.
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