| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Skeletal Muscle Research Laboratory, School of Medical Sciences, RMIT University, Bundoora, Victoria, Australia
* To whom correspondence should be addressed. E-mail: mark.febbraio{at}rmit.edu.au.
To determine the expression and induction of cytokines in human skeletal muscle during concentric contractions, eight males performed 60 min of bicycle exercise, with either a normal (CON) or reduced (LO GLY) pre-exercise intramuscular glycogen content. Muscle biopsy samples were obtained before and after exercise and analyzed for glycogen and the mRNA expression of 13 cytokines. Resting muscle glycogen was higher (P<0.05) in CON compared with LO GLY and was reduced (P<0.05) to 102 ± 32 vs 17 ± 5 mmol glycosyl u.kg-1 dry mass for CON and LO GLY respectively. We detected mRNA levels in human skeletal muscle for 5 cytokines, namely interleukin (IL)-1
, IL-6, IL-8, IL-15, and tumour necrosis factor-
. However, muscle contraction increased (P<0.05) the mRNA expression of IL-6 and IL-8 alone. In addition, the fold change for both IL-8 and IL-6 was markedly higher (P<0.05) in LO GLY compared with CON. Given these results, we analysed venous blood samples, obtained prior to and during exercise, for IL-6 and IL-8. Plasma IL-6 was not different at rest, and although the circulating concentration of this cytokine increased (P<0.05) it increased to a greater extent (P<0.05) throughout exercise in LO GLY. In contrast, plasma IL-8 was not affected by exercise or treatment. These data demonstrate that cytokines are not ubiquitously expressed in skeletal muscle and that only IL-6 and IL-8 mRNA are increased during contraction of this mode and duration. Furthermore, the mRNA abundance of IL-6 and IL-8 appears to be influenced by glycogen availability in the contracting muscle.
This article has been cited by other articles:
|
|
 |
|
  T. Nedachi, H. Fujita, and M. Kanzaki Contractile C2C12 myotube model for studying exercise-inducible responses in skeletal muscle Am J Physiol Endocrinol Metab, November 1, 2008; 295(5): E1191 - E1204. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. K. Pedersen and M. A. Febbraio Muscle as an Endocrine Organ: Focus on Muscle-Derived Interleukin-6 Physiol Rev, October 1, 2008; 88(4): 1379 - 1406. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. S. Mathai, A. Bonen, C. R. Benton, D. L. Robinson, and T. E. Graham Rapid exercise-induced changes in PGC-1{alpha} mRNA and protein in human skeletal muscle J Appl Physiol, October 1, 2008; 105(4): 1098 - 1105. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Broholm, O. H. Mortensen, S. Nielsen, T. Akerstrom, A. Zankari, B. Dahl, and B. K. Pedersen Exercise induces expression of leukaemia inhibitory factor in human skeletal muscle J. Physiol., April 15, 2008; 586(8): 2195 - 2201. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Louis, U. Raue, Y. Yang, B. Jemiolo, and S. Trappe Time course of proteolytic, cytokine, and myostatin gene expression after acute exercise in human skeletal muscle J Appl Physiol, November 1, 2007; 103(5): 1744 - 1751. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. Nielsen, R. Mounier, P. Plomgaard, O. H. Mortensen, M. Penkowa, T. Speerschneider, H. Pilegaard, and B. K. Pedersen Expression of interleukin-15 in human skeletal muscle effect of exercise and muscle fibre type composition J. Physiol., October 1, 2007; 584(1): 305 - 312. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. K. Pedersen, T. C. A. Akerstrom, A. R. Nielsen, and C. P. Fischer Role of myokines in exercise and metabolism J Appl Physiol, September 1, 2007; 103(3): 1093 - 1098. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Frydelund-Larsen, M. Penkowa, T. Akerstrom, A. Zankari, S. Nielsen, and B. K. Pedersen Muscle: Exercise induces interleukin-8 receptor (CXCR2) expression in human skeletal muscle Exp Physiol, January 1, 2007; 92(1): 233 - 240. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. G. Smith and G. E. O. Muscat Orphan nuclear receptors: therapeutic opportunities in skeletal muscle Am J Physiol Cell Physiol, August 1, 2006; 291(2): C203 - C217. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Bruunsgaard Physical activity and modulation of systemic low-level inflammation J. Leukoc. Biol., October 1, 2005; 78(4): 819 - 835. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. C. Ho, M. F. Hirshman, Y. Li, D. Cai, J. R. Farmer, W. G. Aschenbach, C. A. Witczak, S. E. Shoelson, and L. J. Goodyear Regulation of I{kappa}B kinase and NF-{kappa}B in contracting adult rat skeletal muscle Am J Physiol Cell Physiol, October 1, 2005; 289(4): C794 - C801. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Akerstrom, A. Steensberg, P. Keller, C. Keller, M. Penkowa, and B. K. Pedersen Exercise induces interleukin-8 expression in human skeletal muscle J. Physiol., March 1, 2005; 563(2): 507 - 516. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
