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Am J Physiol Regul Integr Comp Physiol (April 29, 2004). doi:10.1152/ajpregu.00071.2004
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Submitted on February 3, 2004
Accepted on April 28, 2004

EFFECT OF ACUTE EXERCISE AND EXERCISE TRAINING ON VEGF SPLICE VARIANTS IN HUMAN SKELETAL MUSCLE

Lotte Jensen1*, Henriette Pilegaard2, P. Darrell Neufer3, and Ylva Hellsten1

1 Copenhagen Muscle Research Centre, Institute for Exercise and Sport Science, Copenhagen, Denmark
2 Copenhagen Muscle Research Centre, The August Krogh Institute, Copenhagen University, Copenhagen, Denmark
3 The John B. Pierce Laboratory and the Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT, USA

* To whom correspondence should be addressed. E-mail: lhoffner{at}ifi.ku.dk.

The present study investigated the effect of an acute exercise bout on the mRNA response of VEGF splice variants in untrained and trained human skeletal muscle. Seven habitually active young men performed one-legged knee-extensor exercise training at an intensity corresponding to approximately 70% of the maximal workload in an incremental test five times a week for four weeks. Biopsies were obtained from m.vastus lateralis of the trained and untrained leg 40h after the last training session. The subjects then performed three hours of two-legged knee-extensor exercise and biopsies were obtained from both legs after 0, 2, 6 and 24 h of recovery. Real-time PCR was used to examine the expression of VEGF mRNA containing exon 1 and 2 (all VEGF isoforms), exon 6 or exon 7 and VEGF165 mRNA. Acute exercise induced an increase (P<0.05) in total VEGF mRNA levels as well as VEGF165 and VEGF splice variants containing exon 7 at 0, 2 and 6 h of recovery. The increase in VEGF mRNA was higher in the untrained than in the trained leg (P<0.05). The results suggest that in human skeletal muscle, acute exercise increases total VEGF mRNA, an increase that appears to be explained mainly by an increase in VEGF165 mRNA. Furthermore, four weeks of training attenuated the exercise-induced response in skeletal muscle VEGF165 mRNA.




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