AJP - Regu Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Regul Integr Comp Physiol (July 13, 2006). doi:10.1152/ajpregu.00153.2006
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
291/6/R1817    most recent
00153.2006v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gu, J.-W.
Right arrow Articles by Manning, Jr., R. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gu, J.-W.
Right arrow Articles by Manning, Jr., R. D.
Submitted on March 10, 2006
Accepted on July 1, 2006

Renal NF{kappa}B Activation and TNF{alpha} Up-regulation Correlate with Salt-sensitive Hypertension in Dahl Salt-sensitive (SS) Rats

Jian-Wei Gu1*, Niu Tian1, Megan Shparago1, Wei Tan1, Amelia P Bailey1, and R. Davis Manning, Jr.1

1 Physiology and Biophysics, University of Mississippi Medical Center, Jackson, Mississippi, United States

* To whom correspondence should be addressed. E-mail: jgu{at}physiology.umsmed.edu.

Molecular mechanisms of salt-sensitive hypertension related to renal inflammation have not been defined. We seek to determine whether a high salt diet induces renal activation of NF{kappa}B and up-regulation of TNF{alpha} related to the development of hypertension in Dahl SS rats. Six, 8-wk-old male Dahl SS rats received a high sodium diet (HS, 4%) and 6 Dahl SS rats received a low sodium diet (LS, 0.3 %) for 5 weeks. In the end, mean arterial pressure (MAP) was determined in conscious rats by continuous monitoring through a catheter placed in the carotid artery. MAP was significantly higher in the HS than the LS group (177.9±3.7 vs. 109.4±2.9 mmHg, P<0.001). There was a significant increase in urinary albumin secretion in the HS group, compared to the LS group (22.3 ±2.6 vs. 6.1±0.7 mg/d; P<0.001). Electrophoretic mobility shift assay (EMSA) demonstrated that the binding activity of NF{kappa}B p65 proteins in the kidneys of Dahl SS rats were significantly increased by 53% in the HS group, compared to the LS group (P=0.007). ELISA indicated that renal protein levels of TNF{alpha}, but not IL-6, interferon-{gamma}, and CCL28, were significantly higher in the HS than the LS group (2.3±0.8 vs. 0.7±0.2 pg/mg; P=0.036). We demonstrated that plasma levels of TNF{alpha} were significantly increased by 5-fold in Dahl SS rats on a high salt diet, compared to a low salt diet. Also, we found that increased physiologically relevant sodium concentration (10 mmol/L) directly stimulated NF{kappa}B activation in cultured human renal proximal tubular epithelial cells. These findings support the hypothesis that activation of NF{kappa}B and up-regulation of TNF{alpha} are the important renal mechanisms linking pro-inflammatory response to salt-sensitive hypertension.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
P. W. Sanders
Vascular consequences of dietary salt intake
Am J Physiol Renal Physiol, August 1, 2009; 297(2): F237 - F243.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
J.-W. Gu, R. D. Manning Jr., E. Young, M. Shparago, B. Sartin, and A. P. Bailey
Vascular endothelial growth factor receptor inhibitor enhances dietary salt-induced hypertension in Sprague-Dawley rats
Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2009; 297(1): R142 - R148.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
P. W. Sanders
Dietary Salt Intake, Salt Sensitivity, and Cardiovascular Health
Hypertension, March 1, 2009; 53(3): 442 - 445.
[Full Text] [PDF]

Home page
 Cardiovasc ResHome page
K. T. Weber, W. B. Weglicki, and R. U. Simpson
Macro- and micronutrient dyshomeostasis in the adverse structural remodelling of myocardium
Cardiovasc Res, February 15, 2009; 81(3): 500 - 508.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
M. Shahid, J. Francis, and D. S. A. Majid
Tumor necrosis factor-{alpha} induces renal vasoconstriction as well as natriuresis in mice
Am J Physiol Renal Physiol, December 1, 2008; 295(6): F1836 - F1844.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
M. M. Diaz Encarnacion, G. M. Warner, C. E. Gray, J. Cheng, H. K. H. Keryakos, K. A. Nath, and J. P. Grande
Signaling pathways modulated by fish oil in salt-sensitive hypertension
Am J Physiol Renal Physiol, June 1, 2008; 294(6): F1323 - F1335.
[Abstract] [Full Text] [PDF]

Home page
 Arch Intern MedHome page
L. J. Appel, J. T. Wright Jr, T. Greene, J. W. Kusek, J. B. Lewis, X. Wang, M. S. Lipkowitz, K. C. Norris, G. L. Bakris, M. Rahman, et al.
Long-term Effects of Renin-Angiotensin System-Blocking Therapy and a Low Blood Pressure Goal on Progression of Hypertensive Chronic Kidney Disease in African Americans
Arch Intern Med, April 28, 2008; 168(8): 832 - 839.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
M. Franco, F. Martinez, Y. Quiroz, O. Galicia, R. Bautista, R. J. Johnson, and B. Rodriguez-Iturbe
Renal angiotensin II concentration and interstitial infiltration of immune cells are correlated with blood pressure levels in salt-sensitive hypertension
Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2007; 293(1): R251 - R256.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 2006 by the American Physiological Society.