| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Articles in PresS, published online ahead of print July 18, 2002
Am J Physiol Regu Physiol, 10.1152/ajpregu.00278.2002
Submitted on May 16, 2002
Accepted on July 9, 2002
1 Departments of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
2 Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
3 Center for Biologic Imaging, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
4 Departments of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA; Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
5 Departments of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA; Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
* To whom correspondence should be addressed. E-mail: finkmp{at}anes.upmc.edu.
We sought to determine whether gut-derived microbial factors influence the hepatic or intestinal inflammatory response to hemorrhagic shock and resuscitation (HS/R). Conventional and gnotobiotic mice contaminated with a defined microbiota without Gram-negative bacteria were subjected to either a sham procedure or HS/R. Tissue samples were obtained 4 h later for assessing ileal mucosal permeability to fluorescein isothiocyanate dextran and hepatic and ileal mucosal steady-state IL-6, iNOS, COX-2 and TNF mRNA levels. Whereas HS/R significantly increased ileal mucosal permeability in conventional mice, this effect was not apparent in gnotobiotic animals. HS/R markedly increased hepatic mRNA levels for several pro-inflammatory genes in both conventional and gnotobiotic mice. HS/R increased ileal mucosal IL-6 and COX-2 mRNA expression in conventional but not gnotobiotic mice. If gnotobiotic mice were contaminated with Escherichia coli C25, HS/R increased ileal mucosal permeability and up-regulated expression of IL-6 and COX-2. These data support the view that the hepatic inflammatory response to HS/R is largely independent of the presence of potentially pathogenic Gram-negative bacteria colonizing the gut, whereas the local mucosal response to HS/R is profoundly influenced by the microbial ecology within the lumen during and shortly after the period of hemorrhage.
This article has been cited by other articles:
|
|
 |
|
  K. P. Mollen, R. M. Levy, J. M. Prince, R. A. Hoffman, M. J. Scott, D. J. Kaczorowski, R. Vallabhaneni, Y. Vodovotz, and T. R. Billiar Systemic inflammation and end organ damage following trauma involves functional TLR4 signaling in both bone marrow-derived cells and parenchymal cells J. Leukoc. Biol., January 1, 2008; 83(1): 80 - 88. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. M. Levy, K. P. Mollen, J. M. Prince, D. J. Kaczorowski, R. Vallabhaneni, S. Liu, K. J. Tracey, M. T. Lotze, D. J. Hackam, M. P. Fink, et al. Systemic inflammation and remote organ injury following trauma require HMGB1 Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2007; 293(4): R1538 - R1544. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Yang, X. Tan, A. M. Thomas, R. Steppacher, N. Qureshi, D. C. Morrison, and C. W. Van Way III Alanine-Glutamine Dipeptide (AGD) Inhibits Expression of Inflammation-Related Genes in Hemorrhagic Shock JPEN J Parenter Enteral Nutr, January 1, 2007; 31(1): 32 - 36. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Yang, X. Tan, A. M. Thomas, J. Shen, N. Qureshi, D. C. Morrison, and C. W. Van Way III Crocetin Inhibits mRNA Expression for Tumor Necrosis Factor-{alpha}, Interleukin-1{beta}, and Inducible Nitric Oxide Synthase in Hemorrhagic Shock JPEN J Parenter Enteral Nutr, July 1, 2006; 30(4): 297 - 301. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Yang, X. Han, T. Uchiyama, S. K. Watkins, A. Yaguchi, R. L. Delude, and M. P. Fink IL-6 is essential for development of gut barrier dysfunction after hemorrhagic shock and resuscitation in mice Am J Physiol Gastrointest Liver Physiol, August 8, 2003; 285(3): G621 - G629. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. L. Sappington, X. Han, R. Yang, R. L. Delude, and M. P. Fink Ethyl Pyruvate Ameliorates Intestinal Epithelial Barrier Dysfunction in Endotoxemic Mice and Immunostimulated Caco-2 Enterocytic Monolayers J. Pharmacol. Exp. Ther., January 1, 2003; 304(1): 464 - 476. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
