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1 INTS, INSERM U76, Paris, 75015, France
2 Broussais Hospital, INSERM U430, Paris, 75015, France
3 CNRS, UMR 7519, Strasbourg, 67084, France
* To whom correspondence should be addressed. E-mail: trinh{at}idf.inserm.fr.
Selective transporters account for rapid urea transport across plasma membrane of several cell types. UT-B1 urea transporter is widely distributed in rat and human tissues. Because mice exhibit high urea turnover and are the preferred species for gene engineering, we have delineated UT-B1 tissue expression in murine tissues. A cDNA was cloned from Balb/C mouse kidney, encoding a polypeptide that differed from C57/bl6 mouse UT-B1 by one residue (Val-8-Ala). UT-B1 mRNA was detected by RT-PCR in brain, kidney, bladder, testis, lung, spleen, and digestive tract (liver, stomach, jejunum, colon). Northern blotting revealed seven UT-B1 transcripts in mouse tissues. Immunoblots identified a non-glycosylated UT-B1 protein of 29-kDa in most tissues, and of 36 and 32 kDa in testis and liver, respectively. UT-B1 protein of gastrointestinal tract did not undergo N-glycosylation. Immunohistochemistry and in situ hybridization localized UT-B1 in urinary tract urothelium (papillary surface, ureter, bladder, and urethra), prominently on plasma membranes, and restricted to the basolateral area in umbrella cells. UT-B1 was found in endothelial cells of descending vasa recta in kidney medulla, and in astrocyte processes in brain. Dehydration induced by water deprivation for 2 days caused a tissue-specific decrease in UT-B1 abundance in the urinary bladder and the ureter.
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