| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Department o Psychiatry, McGill University, Douglas Hospital Research Centre, Montreal, Quebec, Canada
2 Department o Psychiatry, McGill University, Douglas Hospital Research Centre, Montreal, Quebec, Canada; School of Psychological Science, La Trobe University, Bundoora, Australia
3 National Institute for Biological Standards and Control, Potters Bar, United Kingdom
* To whom correspondence should be addressed. E-mail: giamal.luheshi{at}mcgill.ca.
Polyinosinic:polycytidylic acid (poly I:C) is a synthetic double-stranded RNA that is used experimentally to model viral infections in vivo. Previous studies investigating the inflammatory properties of this agent in rodents demonstrated that it is a potent pyrogen. However, the mechanisms underlying this response have not been fully elucidated. In the current study, we examined the effects of peripheral administration of poly I:C on body temperature and cytokine production. Male rats were implanted with biotelemetry devices and randomly assigned to one of three groups: poly I:C + saline, poly I:C + interleukin 1 receptor antagonist (IL-1ra) or saline + saline. Maximal fever of 1.6 °C above baseline was observed 3h after an intraperitoneal (i.p.) injection of poly I:C (750 µg/kg). Pre-treatment with IL-1ra diminished this response by more than 50% (maximum body temperature = 0.6 °C above baseline). Plasma IL-6 concentration increased 5-fold 2h post-poly I:C compared to saline injected rats; levels returned to baseline 4h post-injection. Pre-treatment with IL-1ra prevented this rise in IL-6. Plasma tumor necrosis factor (TNF)-
was also increased more than 4-fold 2h post-injection, but remained unaffected by IL-1ra treatment. IL-1
and cyclooxygenase-2 mRNA were significantly upregulated in the hypothalamus of poly I:C treated animals. Finally, poly I:C decreased food intake by 30%, but this response was not altered by pre-treatment with IL-1ra. These results suggest that poly I:C induces fever, but not anorexia, through an IL-1 and prostaglandin dependent mechanism.
This article has been cited by other articles:
|
|
 |
|
  U. Meyer, J. Feldon, and B. K. Yee A Review of the Fetal Brain Cytokine Imbalance Hypothesis of Schizophrenia Schizophr Bull, September 1, 2009; 35(5): 959 - 972. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. E. P. Smith, J. Li, K. Garbett, K. Mirnics, and P. H. Patterson Maternal Immune Activation Alters Fetal Brain Development through Interleukin-6 J. Neurosci., October 3, 2007; 27(40): 10695 - 10702. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Greis, J. Murgott, S. Rafalzik, R. Gerstberger, T. Hubschle, and J. Roth Characterization of the febrile response induced by fibroblast-stimulating lipopeptide-1 in guinea pigs Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2007; 293(1): R152 - R161. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Ashdown, S. Poole, P. Boksa, and G. N. Luheshi Interleukin-1 receptor antagonist as a modulator of gender differences in the febrile response to lipopolysaccharide in rats Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2007; 292(4): R1667 - R1674. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. S. C. Fabricio, G. Tringali, G. Pozzoli, M. C. Melo, J. A. Vercesi, G. E. P. Souza, and P. Navarra Interleukin-1 mediates endothelin-1-induced fever and prostaglandin production in the preoptic area of rats Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2006; 290(6): R1515 - R1523. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Meyer, M. Nyffeler, A. Engler, A. Urwyler, M. Schedlowski, I. Knuesel, B. K. Yee, and J. Feldon The time of prenatal immune challenge determines the specificity of inflammation-mediated brain and behavioral pathology. J. Neurosci., May 3, 2006; 26(18): 4752 - 4762. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Ellis, A. Mouihate, and Q. J. Pittman Neonatal programming of the rat neuroimmune response: stimulus specific changes elicited by bacterial and viral mimetics J. Physiol., March 15, 2006; 571(3): 695 - 701. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Hubschle, J. Mutze, P. F. Muhlradt, S. Korte, R. Gerstberger, and J. Roth Pyrexia, anorexia, adipsia, and depressed motor activity in rats during systemic inflammation induced by the Toll-like receptors-2 and -6 agonists MALP-2 and FSL-1 Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2006; 290(1): R180 - R187. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Sachot, S. Poole, and G. N Luheshi Circulating leptin mediates lipopolysaccharide-induced anorexia and fever in rats J. Physiol., November 15, 2004; 561(1): 263 - 272. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
