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Articles in PresS, published online ahead of print September 26, 2002
Am J Physiol Regu Physiol, 10.1152/ajpregu.00303.2002
Submitted on May 28, 2002
Accepted on September 23, 2002
1 Institute of Exercise and Sports Sciences, Copenhagen Muscle Research Centre, Copenhagen, Denmark
2 August Krogh Institute, University of Copenhagen, Copenhagen Muscle Research Centre, Copenhagen, Denmark
* To whom correspondence should be addressed. E-mail: cjuel{at}aki.ku.dk.
The present study investigated the localization of ATP sensitive K+ channels (KATP channels) in human skeletal muscle and the functional importance of these channels for human muscle K+ distribution at rest and during muscle activity. Membrane fractionation based on either the giant vesicle technique or the sucrose-gradient technique in combination with Western blotting demonstrated that the KATP channels are mainly located in the sarcolemma. This localization was confirmed by immunohistochemical measurements. With the microdialysis technique it was demonstrated that local application of the KATP channel inhibitor glibenclamide reduced (P<0.05) interstitial K+ at rest from approximately 4.5 to 4.0 mM, whereas the concentration in the control leg remained constant. Glibenclamide had no effect on the interstitial K+ accumulation during knee-extensor exercise at a power output of 60 W. In contrast to what has been found during in vitro conditions, the present study demonstrated that under in vivo conditions the KATP channels are active at rest and contribute to the accumulation of interstitial K+.
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