Short-term blood pressure (BP) variability is limited by the arterial baroreflex. Methods for measuring the spontaneous baroreflex sensitivity (BRS) aim to quantify the gain of the transfer function between BP and pulse interval (PI) or the slope of the linear relationship between parallel BP and PI changes. These frequency-domain (spectral) and time-domain (sequence) techniques were tested in conscious mice equipped with telemetric devices. The autonomic relevance of these indexes was evaluated using pharmacological blockades. The significant changes of the spectral bandwiths resulting from the autonomic blockades were used to identify the low frequency (LF) and high frequency (HF) zones of interest. The LF gain was 1.45 ± 0.14 ms/mmHg, with a phase to PI delay of 0.5 s. For the HF gain, the average values were 2.0 ± 0.19 ms/mmHg, with a null phase. LF and HF bands were markedly affected by atropine. On the 51.2 s segments used for cross spectral analysis, an average number of 26.4 ± 2.2 slopes were detected and the average slope in resting mice was 4.4 ± 0.5 ms/mmHg. Atropine significantly reduced the number of sequences and the slopes. BRS obtained using the sequence technique were highly correlated to the spectral estimates. This study demonstrates the applicability of the recent methods used to estimate spontaneous BRS in mice. There was a vagal predominance in the baroreflex control of HR in conscious mice in the present conditions.