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Am J Physiol Regul Integr Comp Physiol (December 22, 2005). doi:10.1152/ajpregu.00352.2004
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Submitted on June 1, 2004
Accepted on December 19, 2005

Tissue distributions of UT-A and UT-B mRNA and protein in rat

John J Doran1*, Janet D Klein1, Young Hee Kim1, Tekla D Smith1, Shelley D Kozlowski1, and Jeff M Sands2

1 Department of Medicine, Renal Division, Emory University School of Medicine, Atlanta, GA, USA
2 Department of Medicine, Renal Division, Emory University School of Medicine, Atlanta, GA, USA; Department of Physiology, Emory University School of Medicine, Atlanta, GA, USA

* To whom correspondence should be addressed. E-mail: jdora01{at}emory.edu.

Background. Mammalian urea transporters are facilitated membrane transport proteins belonging to two families, UT-A and UT-B. They are best known for their role of maintaining the renal inner medullary urinary concentrating gradient. Urea transporters have also been identified in tissues not typically associated with urea metabolism. The purpose of this study was to survey the major organs in rat to determine the distribution of UT-A and UT-B mRNA transcripts and protein forms and determine their cellular localization. Methods. Five kidney subregions and seventeen extra-renal tissues were screened by northern analysis using two UT-A and three UT-B probes and by western analysis using polyclonal C-terminal UT-A and UT-B antibodies. Immunohistochemistry was performed on sixteen extra-renal tissues using the same antibodies. Results. In kidney we detected mRNA transcripts and protein bands consistent with previously-identified UT-A and UT-B isoforms as well as novel forms. We found that UT-A mRNA and protein are widely expressed in extra-renal tissues in various forms that are different from the known isoforms. We determined the cellular localization of UT-A and UT-B in these tissues. Conclusions. We found that both UT-A and UT-B are ubiquitously expressed as numerous tissue-specific mRNA transcripts and protein forms that are localized to cell membranes, cytoplasm or nuclei.




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