The sphincter mechanism at the esophagogastric junction includes the smooth muscle of the lower esophagus and the skeletal muscle of the crural diaphragm (CD). The smooth muscle is known to be under the control of the dorsal motor nucleus of the vagus (DMV), while CNS control of the CD is unknown. The main purposes of our study were to determine the CNS site that controls the CD, and whether simultaneous changes in lower esophageal sphincter (LES) pressure and CD activity occur when this site is activated. Experiments were performed on anesthetized male ferrets whose LES pressure, CD activity and fundus tone were monitored. To activate DMV neurons, L-glutamate was microinjected unilaterally into the DMV at three areas: intermediate, rostral and caudal. Stimulation of the intermediate DMV decreased CD activity (-4.8±0.1 bursts/min and -0.3±0.01 mV), and LES pressure (-13.2±2.0 mmHg; n=9). Stimulation of this brain site also produced an increase in fundus tone. Stimulation of the rostral DMV elicited increases in the activity of all three target organs (n=5). Stimulation of the caudal DMV had no effect on the CD but did decrease both LES pressure and fundus tone (n=5). All changes in LES pressure, fundus tone, and some DMV-induced changes in CD activity (i.e. bursts/min) were prevented by ipsilateral vagotomy. Our data indicate that simultaneous changes in activity of the esophagogastric sphincters and the fundus tone occur from the rostral and intermediate areas of the DMV, and that these changes are largely mediated by efferent vagus nerves.