AJP - Regu Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Regul Integr Comp Physiol (September 30, 2004). doi:10.1152/ajpregu.00399.2004
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
288/1/R311    most recent
00399.2004v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ji, L. L
Right arrow Articles by Cunningham, J. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ji, L. L
Right arrow Articles by Cunningham, J. T.
Submitted on June 15, 2004
Accepted on September 23, 2004

Effects of water deprivation and rehydration on c-Fos and FosB staining in the rat supraoptic nucleus and lamina terminalis region

Lisa L Ji1, Tiffany Fleming1, Maurice L Penny1, Glenn M Toney2, and J. Thomas Cunningham1*

1 Department of Pharmacology, University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA
2 Department of Physiology, and the Center for Biomedical Neurosciences, University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA

* To whom correspondence should be addressed. E-mail: Cunninghamt{at}uthscsa.edu.

We studied cFos and FosB staining in the supraoptic nucleus (SON) the organum vasculosum of the lamina terminalis (OVLT) and the median preoptic nucleus (MnPO) in adult male rats following water deprivation (24 h, n = 11; 48 h,n = 12) and water deprivation with rehydration (22 h + water, n = 11; 46 h + water, n =10). Control rats (n=15) had water available ad libitum. Separate sets of serial sections from each brain were processed for immunocytochemistry using primary antibodies against either c-Fos or FosB protein. Plasma osmolality, vasopressin, hematocrit and plasma proteins were measured in separate groups (n=6-7). The number of c-Fos-positive cells in the SON was significantly increased after 24 and 48 h water deprivation. In contrast, rehydrated groups were not different from control. Water deprivation significantly increased c-Fos staining in both the OVLT and the MnPO but c-Fos staining was not altered by rehydration. FosB staining in the SON was significantly increased only by 48 h water deprivation and this effect was significantly decreased by rehydration. In the MnPO and OVLT, FosB staining was significantly increased by water deprivation and, like c-Fos staining, these increases were not affected by rehydration. Water deprivation significantly increased osmolality and hematocrit as well as plasma protein and vasopressin concentrations. Plasma measurements from rehydrated rats were not different from control. We conclude that water deprivation and rehydration differentially effect c-Fos and FosB staining in a region dependent manner.




This article has been cited by other articles:


Home page
 J. Physiol.Home page
P. Shi, M. A. Martinez, A. S. Calderon, Q. Chen, J. T. Cunningham, and G. M. Toney
Intra-carotid hyperosmotic stimulation increases Fos staining in forebrain organum vasculosum laminae terminalis neurones that project to the hypothalamic paraventricular nucleus
J. Physiol., November 1, 2008; 586(21): 5231 - 5245.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
C. Wotus, M. M. Arnhold, and W. C. Engeland
Dehydration-induced drinking decreases Fos expression in hypothalamic paraventricular neurons expressing vasopressin but not corticotropin-releasing hormone
Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2007; 292(3): R1349 - R1358.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. Qiu, S. Yao, C. Hindmarch, V. Antunes, J. Paton, and D. Murphy
Transcription Factor Expression in the Hypothalamo-Neurohypophyseal System of the Dehydrated Rat: Upregulation of Gonadotrophin Inducible Transcription Factor 1 mRNA Is Mediated by cAMP-Dependent Protein Kinase A
J. Neurosci., February 28, 2007; 27(9): 2196 - 2203.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
P. J. Mueller, M. J. Sullivan, R. R. Grindstaff, J. T. Cunningham, and E. M. Hasser
Regulation of plasma vasopressin and renin activity in conscious hindlimb-unloaded rats
Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2006; 291(1): R46 - R52.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
H. B. Gottlieb, L. L. Ji, H. Jones, M. L. Penny, T. Fleming, and J. T. Cunningham
Differential effects of water and saline intake on water deprivation-induced c-Fos staining in the rat
Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2006; 290(5): R1251 - R1261.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
L. Rinaman, R. R. Vollmer, J. Karam, D. Phillips, X. Li, and J. A. Amico
Dehydration anorexia is attenuated in oxytocin-deficient mice
Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2005; 288(6): R1791 - R1799.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 2004 by the American Physiological Society.