AJP - Regu Track the topics, authors and articles important to you
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Regul Integr Comp Physiol (October 3, 2002). doi:10.1152/ajpregu.00455.2002
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
284/2/R474    most recent
00455.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Drew, B.
Right arrow Articles by Leeuwenburgh, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Drew, B.
Right arrow Articles by Leeuwenburgh, C.

Articles in PresS, published online ahead of print October 3, 2002
Am J Physiol Regu Physiol, 10.1152/ajpregu.00455.2002
Submitted on July 25, 2002
Accepted on September 30, 2002

Effects of aging and caloric restriction on mitochondrial energy production in gastrocnemius muscle and heart

Barry Drew1, Sharon Phaneuf1, Amie Dirks1, Colin Selman1, Ricardo Gredilla2, Angela Lezza3, Gustavo Barja2, and Christiaan Leeuwenburgh1*

1 Biochemistry of Aging Laboratory, University of Florida, Gainesville, FL, USA
2 Department of Animal Biology-II (Animal Physiology), Complutense University, Madrid, Spain
3 Department of Biochemistry and Molecular Biology, University of Bari, Bari, Italy

* To whom correspondence should be addressed. E-mail: cleeuwen{at}ufl.edu.

Mitochondria are chronically exposed to reactive oxygen intermediates. As a result, various tissues, including skeletal muscle and heart, are characterized by an age-associated increase in reactive oxidant-induced mitochondrial DNA damage. It has been postulated that these alterations may result in a decline in the content and rate of production of adenosine tri-phosphate, which may affect tissue function, contribute to the aging process, and lead to several disease states. We show that with age, ATP content and production decreased by ~50% in isolated rat mitochondria from the gastrocnemius muscle; however, no decline was observed in heart mitochondria. The decline observed in skeletal muscle may be a factor in the process of sarcopenia, which increases in incidence with advancing age. Life long caloric restriction, which prolongs maximum life span in animals, did not attenuate the age-related decline in ATP content or rate of production in skeletal muscle and had no effect on the heart. 8-Oxo-7,8-dihydro-2'-deoxyguanosine in skeletal muscle mtDNA was unaffected by aging but decreased 30% with caloric restriction, suggesting that the mechanisms that decrease oxidative stress in these tissues with caloric restriction are independent from ATP availability. The generation of reactive oxygen species, as indicated by H2O2 production in isolated mitochondria, did not change significantly with age in skeletal muscle or in the heart. Caloric restriction tended to reduce the levels of H2O2 production in the muscle, but not in the heart. These data are the first to show that an age-associated decline in ATP content and rate of ATP production are tissue-specific, in that they occur in skeletal muscle but not heart and that mitochondrial ATP production was unaltered by caloric restriction in both tissues.




This article has been cited by other articles:


Home page
 J. Gerontol. A Biol. Sci. Med. Sci.Home page
P. A. Figueiredo, R. M. Ferreira, H. J. Appell, and J. A. Duarte
Age-Induced Morphological, Biochemical, and Functional Alterations in Isolated Mitochondria From Murine Skeletal Muscle
J. Gerontol. A Biol. Sci. Med. Sci., April 1, 2008; 63(4): 350 - 359.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
S. Judge and C. Leeuwenburgh
Cardiac mitochondrial bioenergetics, oxidative stress, and aging
Am J Physiol Cell Physiol, June 1, 2007; 292(6): C1983 - C1992.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
B. Colom, J. Oliver, P. Roca, and F. J. Garcia-Palmer
Caloric restriction and gender modulate cardiac muscle mitochondrial H2O2 production and oxidative damage
Cardiovasc Res, June 1, 2007; 74(3): 456 - 465.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
G. Johnson, D. Roussel, J.-F. Dumas, O. Douay, Y. Malthiery, G. Simard, and P. Ritz
Influence of intensity of food restriction on skeletal muscle mitochondrial energy metabolism in rats
Am J Physiol Endocrinol Metab, September 1, 2006; 291(3): E460 - E467.
[Abstract] [Full Text] [PDF]

Home page
 J. Gerontol. A Biol. Sci. Med. Sci.Home page
D. J. Baker, A. C. Betik, D. J. Krause, and R. T. Hepple
No decline in skeletal muscle oxidative capacity with aging in long-term calorically restricted rats: effects are independent of mitochondrial DNA integrity.
J. Gerontol. A Biol. Sci. Med. Sci., July 1, 2006; 61(7): 675 - 684.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
C. S. Broome, A. C. Kayani, J. Palomero, W. H. Dillmann, R. Mestril, M. J. Jackson, and A. McArdle
Effect of lifelong overexpression of HSP70 in skeletal muscle on age-related oxidative stress and adaptation after nondamaging contractile activity
FASEB J, July 1, 2006; 20(9): 1549 - 1551.
[Abstract] [Full Text] [PDF]

Home page
 JAMAHome page
L. K. Heilbronn, L. de Jonge, M. I. Frisard, J. P. DeLany, D. E. Larson-Meyer, J. Rood, T. Nguyen, C. K. Martin, J. Volaufova, M. M. Most, et al.
Effect of 6-Month Calorie Restriction on Biomarkers of Longevity, Metabolic Adaptation, and Oxidative Stress in Overweight Individuals: A Randomized Controlled Trial
JAMA, April 5, 2006; 295(13): 1539 - 1548.
[Abstract] [Full Text] [PDF]

Home page
 J. Gerontol. A Biol. Sci. Med. Sci.Home page
J. M. Dhahbi, T. Tsuchiya, H.-J. Kim, P. L. Mote, and S. R. Spindler
Gene expression and physiologic responses of the heart to the initiation and withdrawal of caloric restriction.
J. Gerontol. A Biol. Sci. Med. Sci., March 1, 2006; 61(3): 218 - 231.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
S. Judge, Y. M. Jang, A. Smith, C. Selman, T. Phillips, J. R. Speakman, T. Hagen, and C. Leeuwenburgh
Exercise by lifelong voluntary wheel running reduces subsarcolemmal and interfibrillar mitochondrial hydrogen peroxide production in the heart
Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2005; 289(6): R1564 - R1572.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
L. Bevilacqua, J. J. Ramsey, K. Hagopian, R. Weindruch, and M.-E. Harper
Long-term caloric restriction increases UCP3 content but decreases proton leak and reactive oxygen species production in rat skeletal muscle mitochondria
Am J Physiol Endocrinol Metab, September 1, 2005; 289(3): E429 - E438.
[Abstract] [Full Text] [PDF]

Home page
 J. Gerontol. A Biol. Sci. Med. Sci.Home page
A. J. Lambert and B. J. Merry
Lack of Effect of Caloric Restriction on Bioenergetics and Reactive Oxygen Species Production in Intact Rat Hepatocytes
J. Gerontol. A Biol. Sci. Med. Sci., February 1, 2005; 60(2): 175 - 180.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
A. A. Gonzalez, R. Kumar, J. D. Mulligan, A. J. Davis, and K. W. Saupe
Effects of aging on cardiac and skeletal muscle AMPK activity: basal activity, allosteric activation, and response to in vivo hypoxemia in mice
Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2004; 287(5): R1270 - R1275.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
K. R. Short, B. Drew, and C. Leeuwenburgh
Mitochondrial ATP measurements
Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2004; 287(1): R243 - R246.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
L. Bevilacqua, J. J. Ramsey, K. Hagopian, R. Weindruch, and M.-E. Harper
Effects of short- and medium-term calorie restriction on muscle mitochondrial proton leak and reactive oxygen species production
Am J Physiol Endocrinol Metab, June 1, 2004; 286(5): E852 - E861.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
D. J. Marcinek, K. A. Schenkman, W. A. Ciesielski, and K. E. Conley
Mitochondrial coupling in vivo in mouse skeletal muscle
Am J Physiol Cell Physiol, February 1, 2004; 286(2): C457 - C463.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
D. Julian and C. Leeuwenburgh
Linkage between insulin and the free radical theory of aging
Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2004; 286(1): R20 - R21.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
A. J. Lambert and B. J. Merry
Effect of caloric restriction on mitochondrial reactive oxygen species production and bioenergetics: reversal by insulin
Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2004; 286(1): R71 - R79.
[Abstract] [Full Text]

Home page
 J. Appl. Physiol.Home page
A. M. Payne, S. L. Dodd, and C. Leeuwenburgh
Life-long calorie restriction in Fischer 344 rats attenuates age-related loss in skeletal muscle-specific force and reduces extracellular space
J Appl Physiol, December 1, 2003; 95(6): 2554 - 2562.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
B. Drew and C. Leeuwenburgh
Method for measuring ATP production in isolated mitochondria: ATP production in brain and liver mitochondria of Fischer-344 rats with age and caloric restriction
Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2003; 285(5): R1259 - R1267.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 2002 by the American Physiological Society.