| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Anatomy, The Catholic University of Korea, Seoul, Korea, Republic of; Cell Death Disease Research Center, The Catholic University of Korea, Seoul, Korea, Republic of
2 Anatomy, Ewha Womans University, Seoul, Korea, Republic of
3 Internal Medicine, The Catholic University of Korea, Seoul, Korea, Republic of; Cell Death Disease Research Center, The Catholic University of Korea, Seoul, Korea, Republic of
4 Renal Division, Department of Medicine, Emory University School of Medicine, Atlanta, Georgia, USA
5 Laboratory of Kidney and Electrolyte Metabolism, National Institutes of Health, Bethesda, Maryland, USA
6 Department of Medicine, University of Florida College of Medicine, Gainesville, Florida, USA
* To whom correspondence should be addressed. E-mail: jinkim{at}catholic.ac.kr.
Urea transport in the kidney is mediated by a family of transporter proteins, which includes the renal urea transporters (UT-A) and the erythrocyte urea transporter (UT-B). The aim of this study was to determine whether hydration status affects the subcellular distribution of urea transporters. Male SD rats were divided into three groups: dehydrated rats (WD) were given minimum water, hydrated rats (WL) were given 3% sucrose in water for three days before being killed, and control rats had free access to water. Sections of kidneys were labeled with antibodies against UT-A1 and UT-A2 (L194), UT-A3 (Q2), and UT-B using preembedding immunoperoxidase and immunogold methods. In control animals, UT-A1 and UT-A3 immunoreactivity was observed throughout the cytoplasm in the IMCD cells, and weak labeling was observed on the basolateral plasma membrane. UT-A2 immunoreactivity in the DTL was observed mainly on the apical and basolateral membrane of type I epithelium, and very faint labeling was observed in the long-loop DTL at the border between the OM and IM. The intensity of UT-A1 immunoreactivity was markedly lower and UT-A3 immunoreactivity higher in the IMCD in WD than in control. The intensity of UT-A2 immunoreactivity in the plasma membrane and cytoplasm of type I, II (in the lower part of ISOM), and type III (in the initial part of IM) epithelia of DTL was greater in WD than in controls. In contrast, the expression of UT-A1 was greater and the expression of UT-A2 and UT-A3 was lower in WL than in controls. The subcellular distribution of UT-A in the DTL or IMCD did not differ between the control and experimental animals. UT-B was expressed in the plasma membrane of the descending vasa recta of both control and experimental animals. The intensity of UT-B was higher in the kidney from WD and lower in WL than in controls. These data indicate that changes in hydration status over three days affected the expression of the urea transporter protein without changing its subcellular distribution.
This article has been cited by other articles:
|
|
 |
|
  T. L. Pannabecker, W. H. Dantzler, H. E. Layton, and A. T. Layton Role of three-dimensional architecture in the urine concentrating mechanism of the rat renal inner medulla Am J Physiol Renal Physiol, November 1, 2008; 295(5): F1271 - F1285. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Blount, J. D. Klein, C. F. Martin, D. Tchapyjnikov, and J. M. Sands Forskolin stimulates phosphorylation and membrane accumulation of UT-A3 Am J Physiol Renal Physiol, October 1, 2007; 293(4): F1308 - F1313. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. S. Stewart, S. L. King, E. A. Potter, and C. P. Smith Acute regulation of mUT-A3 urea transporter expressed in a MDCK cell line Am J Physiol Renal Physiol, April 1, 2007; 292(4): F1157 - F1163. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. J. Doran, J. D. Klein, Y. H. Kim, T. D. Smith, S. D. Kozlowski, R. B. Gunn, and J. M. Sands Tissue distribution of UT-A and UT-B mRNA and protein in rat Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2006; 290(5): R1446 - R1459. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
