Recent studies have indicated that plantar based vibration may be an effective approach for the prevention and treatment of osteoporosis. We addressed the hypothesis of whether the plantar vibration operated by way of the skeletal muscle pump, resulting in enhanced blood and fluid flow to the lower body. We combined plantar stimulation with upright tilt table testing in 18 women aged 46-63 years. We used strain gauge plethysmography to measure calf blood flow, venous capacitance, and the microvascular filtration relation, and impedance plethysmography to examine changes in leg, splanchnic and thoracic blood flow while supine and during 35° upright tilt. A vibrating platform was placed on the footboard of a tilt table and measurements made at 0, 15 and 45 Hz with an amplitude of 0.2g p-p, presented in random order. Impedance measured supine blood flows were significantly (p=0.05) increased in the calf (30%), pelvic (26%) and thoracic regions (20%) by plantar vibration at 45 Hz. Moreover, the 25-35% decreases in calf and pelvic blood flows associated with upright tilt were reversed by plantar vibration, and the decrease in thoracic blood flow was significantly attenuated. Strain gauge measurements showed an attenuation of upright calf blood flow. In addition, the microvascular filtration relation was shifted with vibration producing a pronounced increase in the threshold for edema, P_i, due to enhanced lymphatic flow. Supine values for P_i increased from 24±2 mmHg at 0 Hz, to 27±3 mmHg at 15 Hz, and finally to 31±2 mmHg at 45 Hz, (p<.01). Upright values for P_i increased from 25±3 mmHg at 0 Hz, to 28±4 mmHg at 15 Hz, and finally to 35±4 mmHg at 45 Hz. The results suggest that plantar vibration serves to significantly enhance peripheral and systemic blood flow, peripheral lymphatic flow and venous drainage, which may account for the apparent ability of such stimuli to influence bone mass.