AJP - Regu Journal of Neurophysiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Regul Integr Comp Physiol (October 21, 2004). doi:10.1152/ajpregu.00520.2004
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
288/2/R511    most recent
00520.2004v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Douglas, R M
Right arrow Articles by Haddad, G G
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Douglas, R M
Right arrow Articles by Haddad, G G
Submitted on August 3, 2004
Accepted on December 31, 1969

Hypoxia induces major effects on cell cycle kinetics and protein expression in Drosophila melanogaster embryos

R M Douglas1, R Farahani1, P Morcillo1, A Kanaan1, T Xu2, and G G Haddad3*

1 Department of Pediatrics, Albert Einstein College of Medicine, Bronx, NY, USA
2 Department of Genetics, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, CT, USA; The Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT, USA
3 Department of Pediatrics, Albert Einstein College of Medicine, Bronx, NY, USA; Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY, USA

* To whom correspondence should be addressed. E-mail: ghaddad{at}aecom.yu.edu.

Hypoxia induces a stereotypic response in Drosophila melanogaster embryos: depending on the time of hypoxia, embryos arrest cell cycle activity either at metaphase or just prior to S phase. To understand the mechanisms underlying hypoxia-induced arrest, two kinds of experiments were conducted. First, embryos carrying a kinesin-GFP construct, which permits in-vivo confocal microscopic visualization of the cell cycle, showed a dose-response relation between O2 level and cell cycle length. For example, while mild hypoxia (PO2 ~55 Torr) had no apparent effect on cell cycle length (1006±26 sec), while severe hypoxia (PO2 ~25-35 Torr) or anoxia (PO2 = 0 Torr) arrested the cell cycle. Second, we utilized Drosophila embryos carrying a heat shock promoter driving the string (cdc25) gene (HS-STG3), which permits synchronization of embryos prior to the start of mitosis. Under conditions of anoxia, we induced a stabilization or an increase in the expression of several G1/S (e.g.: dE2F1, RBF2) and G2/M (e.g.: cyclin A, cyclin B, dWee1) proteins. This study suggests that in fruit fly embryos: a) there is a dose-dependent relationship between cell cycle length and O2 levels in fruit fly embryos and b) stabilized cyclin A and E2F1 are likely to be the mediators of hypoxia-induced arrest at metaphase and pre-S phase.




This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
R. Pandey, S. Heeger, and C. F. Lehner
Rapid effects of acute anoxia on spindle kinetochore interactions activate the mitotic spindle checkpoint
J. Cell Sci., August 15, 2007; 120(16): 2807 - 2818.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
J. Sollid, A. Kjernsli, P. M. De Angelis, A. K. Rohr, and G. E. Nilsson
Cell proliferation and gill morphology in anoxic crucian carp
Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2005; 289(4): R1196 - R1201.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 2004 by the American Physiological Society.