AJP - Regu Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Regul Integr Comp Physiol (October 24, 2002). doi:10.1152/ajpregu.00570.2002
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
284/3/R698    most recent
00570.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ivanov, A. I
Right arrow Articles by Romanovsky, A. A
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ivanov, A. I
Right arrow Articles by Romanovsky, A. A

Articles in PresS, published online ahead of print October 24, 2002
Am J Physiol Regu Physiol, 10.1152/ajpregu.00570.2002
Submitted on September 16, 2002
Accepted on October 22, 2002

Expression of genes controlling transport and catabolism of prostaglandin E2 in lipopolysaccharide fever

Andrei I Ivanov1, Adrienne C Scheck2, and Andrej A Romanovsky1*

1 Trauma Research, St. Joseph's Hospital and Medical Center, Phoenix, Arizona, USA
2 Barrow Neurological Institute, Neurology Research, St. Joseph's Hospital and Medical Center, Phoenix, Arizona, USA

* To whom correspondence should be addressed. E-mail: aromano{at}chw.edu.

Prostaglandin (PG) E2 is a principal downstream mediator of fever and other symptoms of systemic inflammation. Its inactivation occurs in peripheral tissues, primarily the lungs and liver, via carrier-mediated cellular uptake and enzymatic oxidation. We hypothesized that inactivation of PGE2 is suppressed during lipopolysaccharide (LPS) fever, and that transcriptional down-regulation of PGE2 carriers and catabolizing enzymes contributes to this suppression. Fever was induced in inbred Wistar Kyoto rats by intravenous LPS (50 µg/kg); the controls received saline. Samples of the liver, lungs, and hypothalamus were harvested 0, 0.5, 1.5, and 5 h postinjection. The expression of the two principal transmembrane PGE2 carriers (PG transporter and multispecific organic anion transporter) and the two key PGE2-inactivating enzymes [15-hydroxy-PG dehydrogenase (15-PGDH) and carbonyl reductase] was quantified by RT-PCR. All four genes of interest were down-regulated in peripheral tissues (but not the brain) during fever. Most remarkably, the expression of hepatic 15-PGDH was decreased 26 fold 5 h post-LPS, whereas expression of pulmonary 15-PGDH was down-regulated (as much as 18 fold) throughout the entire febrile course. The transcriptional down-regulation of several proteins involved in PGE2 inactivation, first reported here, is an unrecognized mechanism of systemic inflammation. By increasing the blood-brain gradient of PGE2, this mechanism likely facilitates penetration of PGE2 into the brain and prevents its elimination from the brain.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
A. A. Steiner, J. C. Hunter, S. M. Phipps, T. B. Nucci, D. L. Oliveira, J. L. Roberts, A. C. Scheck, D. L. Simmons, and A. A. Romanovsky
Cyclooxygenase-1 or -2--which one mediates lipopolysaccharide-induced hypothermia?
Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2009; 297(2): R485 - R494.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
Y. Ootsuka, W. W. Blessing, A. A. Steiner, and A. A. Romanovsky
Fever response to intravenous prostaglandin E2 is mediated by the brain but does not require afferent vagal signaling
Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2008; 294(4): R1294 - R1303.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
A. I. Ivanov, A. A. Steiner, A. C. Scheck, and A. A. Romanovsky
Expression of Eph receptors and their ligands, ephrins, during lipopolysaccharide fever in rats
Physiol Genomics, April 14, 2005; 21(2): 152 - 160.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
A. S. C. Fabricio, F. H. Veiga, R. Cristofoletti, P. Navarra, and G. E. P. Souza
The effects of selective and nonselective cyclooxygenase inhibitors on endothelin-1-induced fever in rats
Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2005; 288(3): R671 - R677.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
A. A. Romanovsky
Anorexia: the toll for lipopolysaccharide recognition
Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2004; 287(2): R274 - R275.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
A. A. Romanovsky and S. R. Petersen
The spleen: another mystery about its function
Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2003; 284(6): R1378 - R1379.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
A. I. Ivanov, A. A. Romanovsky, K. Matsumura, A. Mouihate, M. S. Clerget-Froidevaux, J. L. Wallace, and Q. J. Pittman
Near-term suppression of fever: inhibited synthesis or accelerated catabolism of prostaglandin E2?
Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2003; 284(3): R860 - R865.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 2002 by the American Physiological Society.