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Articles in PresS, published online ahead of print October 17, 2002
Am J Physiol Regu Physiol, 10.1152/ajpregu.00573.2002
Submitted on September 16, 2002
Accepted on October 9, 2002
1 Physiology and Neurobiology, University of Connecticut, Storrs, CT, USA
* To whom correspondence should be addressed. E-mail: larry.renfro{at}uconn.ued.
Sulfate (SO42-) transport by winter flounder intestine in Ussing chambers was characterized. With 50 mM SO42- (physiological level) bathing the lumen, net absorption (lumen-to-blood) dominated. Under short-circuited conditions, 1 mM SO42- on both sides, net active SO42- secretion occurred (8.55 ± 0.96 nmoles x cm-2 x hr-1). NaCN (10 mM), ouabain (10-4 M), and luminal 4,4'diisothiocyanatostilbene-2-2'disulfonic acid (DIDS, 0.2 mM) inhibited net secretion. Removal of luminal Cl- and HCO3- together (Cl--HCO3-) or Cl- alone blocked net secretion whereas removal of luminal HCO3- alone increased net secretion. Sulfate uptake into foregut brush-border membrane vesicles was stimulated by a trans-Cl- gradient (in>out), and unaffected by a trans-HCO3- gradient (in>out). Short-circuiting with K+, in=out, and valinomycin had no effect on Cl--stimulated SO42- uptake suggesting electroneutral exchange. Satiety (i.e., full stomach) stimulated the unidirectional absorptive flux, eliminating net secretion. This intestinal Cl-/SO42- exchanger provides new insight into the intestine's role in teleost osmoregulation. It was concluded that the intestine is a site of SO42- absorption in marine teleosts and that active SO42- secretion is in exchange for luminal Cl-.
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