| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
tp6v1) impairs acid secretion and ion balance in zebrafish Danio rerio
1 Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan - Republic of China; Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan - Republic of China
2 Department of Life Science, National Taiwan Normal University, Taipei, Taiwan - Republic of China
3 Institute of Cellular and Organismic Biology , Academia Sinica, Taipei, Taiwan - Republic of China; Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan - Republic of China
4 Department of Biology, St. Francis Xavier University, Antigonish, Canada
5 Deparetment of Aquatic Bioscience, University of Tokyo, Tokyo, Japan
* To whom correspondence should be addressed. E-mail: pphwang{at}gate.sinica.edu.tw.
In the skin of zebrafish embryo, the vacuolar H+-ATPase (V-ATPase, H+ pump) distributed mainly in the apical membrane of H+-pump rich cells, which pump internal acid out of the embryo and function similar to acid-secreting intercalated cells in mammalian kidney. In addition to acid excretion, the electrogenic H+ efflux via the H+-ATPases in the gill apical membrane of freshwater fish was proposed to act as a driving force for Na+ entry through the apical Na+ channels. However, convincing molecular physiological evidence in vivo for this model is still lacking. In this study, we used morpholino-modified antisense oligo-nucleotides to knockdown the gene product of H+-ATPase subunit A (
tp6v1) and examined the phenotype of the mutants. The H+-ATP knockdown embryos revealed several abnormalities, including suppression of acid-secretion from skin, growth retardation, trunk deformation, and loss of internal Ca2+ and Na+. This finding reveals the critical role of H+-ATPase in embryonic acid-secretion and ion balance as well.
This article has been cited by other articles:
|
|
 |
|
  M. H. Braun, S. L. Steele, and S. F. Perry The responses of zebrafish (Danio rerio) to high external ammonia and urea transporter inhibition: nitrogen excretion and expression of rhesus glycoproteins and urea transporter proteins J. Exp. Biol., December 1, 2009; 212(23): 3846 - 3856. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-C. Tseng, R.-D. Chen, J.-R. Lee, S.-T. Liu, S.-J. Lee, and P.-P. Hwang Specific expression and regulation of glucose transporters in zebrafish ionocytes Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2009; 297(2): R275 - R290. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P.-P. Hwang Ion uptake and acid secretion in zebrafish (Danio rerio) J. Exp. Biol., June 1, 2009; 212(11): 1745 - 1752. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B.-K. Liao, R.-D. Chen, and P.-P. Hwang Expression regulation of Na+-K+-ATPase {alpha}1-subunit subtypes in zebrafish gill ionocytes Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2009; 296(6): R1897 - R1906. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-F. Wang, Y.-C. Tseng, J.-J. Yan, J. Hiroi, and P.-P. Hwang Role of SLC12A10.2, a Na-Cl cotransporter-like protein, in a Cl uptake mechanism in zebrafish (Danio rerio) Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2009; 296(5): R1650 - R1660. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. H. Braun, S. L. Steele, M. Ekker, and S. F. Perry Nitrogen excretion in developing zebrafish (Danio rerio): a role for Rh proteins and urea transporters Am J Physiol Renal Physiol, May 1, 2009; 296(5): F994 - F1005. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. S. Flynt, E. J. Thatcher, K. Burkewitz, N. Li, Y. Liu, and J. G. Patton miR-8 microRNAs regulate the response to osmotic stress in zebrafish embryos J. Cell Biol., April 6, 2009; 185(1): 115 - 127. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-L. Horng, L.-Y. Lin, and P.-P. Hwang Functional regulation of H+-ATPase-rich cells in zebrafish embryos acclimated to an acidic environment Am J Physiol Cell Physiol, April 1, 2009; 296(4): C682 - C692. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.-J. Chang, J.-L. Horng, J.-J. Yan, C.-D. Hsiao, and P.-P. Hwang The transcription factor, glial cell missing 2, is involved in differentiation and functional regulation of H+-ATPase-rich cells in zebrafish (Danio rerio) Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2009; 296(4): R1192 - R1201. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. K. Parks, M. Tresguerres, and G. G. Goss Cellular mechanisms of Cl- transport in trout gill mitochondrion-rich cells Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2009; 296(4): R1161 - R1169. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T.-H. Shih, J.-L. Horng, P.-P. Hwang, and L.-Y. Lin Ammonia excretion by the skin of zebrafish (Danio rerio) larvae Am J Physiol Cell Physiol, December 1, 2008; 295(6): C1625 - C1632. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Hiroi, S. Yasumasu, S. D. McCormick, P.-P. Hwang, and T. Kaneko Evidence for an apical Na-Cl cotransporter involved in ion uptake in a teleost fish J. Exp. Biol., August 15, 2008; 211(16): 2584 - 2599. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. H. Evans Teleost fish osmoregulation: what have we learned since August Krogh, Homer Smith, and Ancel Keys Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2008; 295(2): R704 - R713. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T.-Y. Lin, B.-K. Liao, J.-L. Horng, J.-J. Yan, C.-D. Hsiao, and P.-P. Hwang Carbonic anhydrase 2-like a and 15a are involved in acid-base regulation and Na+ uptake in zebrafish H+-ATPase-rich cells Am J Physiol Cell Physiol, May 1, 2008; 294(5): C1250 - C1260. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-J. Yan, M.-Y. Chou, T. Kaneko, and P.-P. Hwang Gene expression of Na+/H+ exchanger in zebrafish H+-ATPase-rich cells during acclimation to low-Na+ and acidic environments Am J Physiol Cell Physiol, December 1, 2007; 293(6): C1814 - C1823. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
