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tp6v1) impairs acid secretion and ion balance in zebrafish Danio rerio
1 Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan - Republic of China; Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan - Republic of China
2 Department of Life Science, National Taiwan Normal University, Taipei, Taiwan - Republic of China
3 Institute of Cellular and Organismic Biology , Academia Sinica, Taipei, Taiwan - Republic of China; Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan - Republic of China
4 Department of Biology, St. Francis Xavier University, Antigonish, Canada
5 Deparetment of Aquatic Bioscience, University of Tokyo, Tokyo, Japan
* To whom correspondence should be addressed. E-mail: pphwang{at}gate.sinica.edu.tw.
In the skin of zebrafish embryo, the vacuolar H+-ATPase (V-ATPase, H+ pump) distributed mainly in the apical membrane of H+-pump rich cells, which pump internal acid out of the embryo and function similar to acid-secreting intercalated cells in mammalian kidney. In addition to acid excretion, the electrogenic H+ efflux via the H+-ATPases in the gill apical membrane of freshwater fish was proposed to act as a driving force for Na+ entry through the apical Na+ channels. However, convincing molecular physiological evidence in vivo for this model is still lacking. In this study, we used morpholino-modified antisense oligo-nucleotides to knockdown the gene product of H+-ATPase subunit A (
tp6v1) and examined the phenotype of the mutants. The H+-ATP knockdown embryos revealed several abnormalities, including suppression of acid-secretion from skin, growth retardation, trunk deformation, and loss of internal Ca2+ and Na+. This finding reveals the critical role of H+-ATPase in embryonic acid-secretion and ion balance as well.
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