| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Articles in PresS, published online ahead of print March 7, 2002
Am J Physiol Regu Physiol, 10.1152/ajpregu.00664.2001
Submitted on November 7, 2001
Accepted on February 26, 2002
1 Department of Physiology, Medical College of Wisconsin, Milwaukee, WI, USA
* To whom correspondence should be addressed. E-mail: magdalena_alonsogalicia{at}merck.com.
The present study examined the effects of angiotensin II (Ang II) on the renal synthesis of 20-HETE and its contribution to the renal vasoconstrictor and the acute and chronic pressor effects of Ang II in rats. Ang II (10-11 to 10-7 mol/L) reduced the diameter of renal interlobular arteries treated with inhibitors of nitric oxide synthase and cyclooxygenase, lipoxygenase and epoxygenase by 81 ± 8%. Subsequent blockade of the synthesis of 20-HETE with 17-octadecynoic acid (17-ODYA, 1 µmol/l) increased the ED50 for Ang II-induced constriction by a factor of 15, and diminished the maximal response by 61%. Graded IV infusion of Ang II (5-200 ng/min) dose-dependently increased mean arterial pressure (MAP) in thiobutylbarbitol anesthetized rats by 35 mmHg. Acute blockade of the formation of 20-HETE with dibromododecenyl methylsulfimide (DDMS, 10 mg.kg-1) attenuated the pressor response to Ang II by 40%. An IV infusion of Ang II (50 ng.kg-1.min-1) in rats for five days increased the formation of 20-HETE and EETs in renal cortical microsomes by 60 and 400%, respectively, and increased MAP by 78 mm Hg. Chronic blockade of the synthesis of 20-HETE with intravenous infusion of DDMS (1 mg.kg-1.hr-1) or EETs and 20-HETE with 1-aminobenzotriazole (ABT, 2.2 mg.kg-1.hr-1) attenuated the Ang II-induced rise in MAP by 40%. Control urinary excretion of 20-HETE averaged 350 ± 23 ng/day and increased to 1020 ± 105 ng/day in rats infused with Ang II (50 ng.kg-1.min-1) for 5 days. In contrast, urinary excretion of 20-HETE only rose to 400 ± 40 and 600 ± 25 ng/day in rats chronically treated with Ang II and ABT or DDMS. These results suggest that acute and chronic elevations in circulating Ang II levels increases the formation of 20-HETE in the kidney and peripheral vasculature and that 20-HETE contributes to the acute and chronic pressor effects of Ang II.
This article has been cited by other articles:
|
|
 |
|
  K. Inoue, K. Sodhi, N. Puri, K. H. Gotlinger, J. Cao, R. Rezzani, J. R. Falck, N. G. Abraham, and M. Laniado-Schwartzman Endothelial-specific CYP4A2 overexpression leads to renal injury and hypertension via increased production of 20-HETE Am J Physiol Renal Physiol, October 1, 2009; 297(4): F875 - F884. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Ponnuchamy and R. A. Khalil Cellular mediators of renal vascular dysfunction in hypertension Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2009; 296(4): R1001 - R1018. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Williams, A. Sarkis, K. M. Hoagland, K. Fredrich, R. P. Ryan, C. Moreno, B. Lopez, J. Lazar, F. J. Fenoy, M. Sharma, et al. Transfer of the CYP4A region of chromosome 5 from Lewis to Dahl S rats attenuates renal injury Am J Physiol Renal Physiol, December 1, 2008; 295(6): F1764 - F1777. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Sugimoto, H. Akasaka, T. Katsuya, K. Node, T. Fujisawa, I. Shimaoka, O. Yasuda, M. Ohishi, T. Ogihara, K. Shimamoto, et al. A Polymorphism Regulates CYP4A11 Transcriptional Activity and Is Associated With Hypertension in a Japanese Population Hypertension, December 1, 2008; 52(6): 1142 - 1148. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. G. Abraham Gene Targeting and Heme Oxygenase-1 Expression in Prevention of Hypertension Induced by Angiotensin II Hypertension, October 1, 2008; 52(4): 618 - 620. [Full Text] [PDF]
|
|
|
|
|
|
P. Minuz, H. Jiang, C. Fava, L. Turolo, S. Tacconelli, M. Ricci, P. Patrignani, A. Morganti, A. Lechi, and J. C. McGiff Altered Release of Cytochrome P450 Metabolites of Arachidonic Acid in Renovascular Disease Hypertension, May 1, 2008; 51(5): 1379 - 1385. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Singh, J. Cheng, H. Deng, R. Kemp, T. Ishizuka, A. Nasjletti, and M. L. Schwartzman Vascular Cytochrome P450 4A Expression and 20-Hydroxyeicosatetraenoic Acid Synthesis Contribute to Endothelial Dysfunction in Androgen-Induced Hypertension Hypertension, July 1, 2007; 50(1): 123 - 129. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. J. Marvar, J. R. Falck, and M. A. Boegehold High dietary salt reduces the contribution of 20-HETE to arteriolar oxygen responsiveness in skeletal muscle Am J Physiol Heart Circ Physiol, March 1, 2007; 292(3): H1507 - H1515. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Williams, A. Sarkis, B. Lopez, R. P. Ryan, A. K. Flasch, and R. J. Roman Elevations in Renal Interstitial Hydrostatic Pressure and 20-Hydroxyeicosatetraenoic Acid Contribute to Pressure Natriuresis Hypertension, March 1, 2007; 49(3): 687 - 694. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Magnusson, C. M. Sorensen, T. H. Braunstein, N.-H. Holstein-Rathlou, and M. Salomonsson Renovascular BKCa channels are not activated in vivo under resting conditions and during agonist stimulation Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2007; 292(1): R345 - R353. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Joly, R. Seqqat, B. Flamion, N. Caron, A. Michel, J. D. Imig, and R. Kramp Increased renal vascular reactivity to ANG II after unilateral nephrectomy in the rat involves 20-HETE Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2006; 291(4): R977 - R986. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Qin, H. Kwansa, E. Bucci, R. J. Roman, and R. C. Koehler Role of 20-HETE in the pial arteriolar constrictor response to decreased hematocrit after exchange transfusion of cell-free polymeric hemoglobin J Appl Physiol, January 1, 2006; 100(1): 336 - 342. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Takeuchi, N. Miyata, M. Renic, D. R. Harder, and R. J. Roman Hemoglobin, NO, and 20-HETE interactions in mediating cerebral vasoconstriction following SAH Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2006; 290(1): R84 - R89. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Sarkis, O. Ito, T. Mori, M. Kohzuki, S. Ito, J. Verbalis, A. W. Cowley Jr., and R. J. Roman Cytochrome P-450-dependent metabolism of arachidonic acid in the kidney of rats with diabetes insipidus Am J Physiol Renal Physiol, December 1, 2005; 289(6): F1333 - F1340. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Takeuchi, M. Renic, Q. C. Bohman, D. R. Harder, N. Miyata, and R. J. Roman Reversal of delayed vasospasm by an inhibitor of the synthesis of 20-HETE Am J Physiol Heart Circ Physiol, November 1, 2005; 289(5): H2203 - H2211. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Huang, Y. Zhou, V. T. Raju, J. Du, H.-H. Chang, C.-Y. Wang, M. W. Brands, J. R. Falck, and M.-H. Wang Renal 20-HETE inhibition attenuates changes in renal hemodynamics induced by L-NAME treatment in pregnant rats Am J Physiol Renal Physiol, November 1, 2005; 289(5): F1116 - F1122. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. You, E. M. Golding, and R. M. Bryan Jr. Arachidonic acid metabolites, hydrogen peroxide, and EDHF in cerebral arteries Am J Physiol Heart Circ Physiol, September 1, 2005; 289(3): H1077 - H1083. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. A. Parker, T. R. Grover, J. P. Kinsella, J. R. Falck, and S. H. Abman Inhibition of 20-HETE abolishes the myogenic response during NOS antagonism in the ovine fetal pulmonary circulation Am J Physiol Lung Cell Mol Physiol, August 1, 2005; 289(2): L261 - L267. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. A. Yaghini, C. Zhang, J.-H. Parmentier, A. M. Estes, N. Jafari, S. A. Schaefer, and K. U. Malik Contribution of Arachidonic Acid Metabolites Derived Via Cytochrome P4504A to Angiotensin II-Induced Neointimal Growth Hypertension, June 1, 2005; 45(6): 1182 - 1187. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Shah Role of the renin-angiotensin system in the pathogenesis of preeclampsia Am J Physiol Renal Physiol, April 1, 2005; 288(4): F614 - F625. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Wang, R. J. Roman, J. R. Falck, L. de la Cruz, and J. H. Lombard Effects of high-salt diet on CYP450-4A {omega}-hydroxylase expression and active tone in mesenteric resistance arteries Am J Physiol Heart Circ Physiol, April 1, 2005; 288(4): H1557 - H1565. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Vera, M. Taylor, Q. Bohman, A. Flasch, R. J. Roman, and D. E. Stec Fenofibrate Prevents the Development of Angiotensin II-Dependent Hypertension in Mice Hypertension, April 1, 2005; 45(4): 730 - 735. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. A. Dos Santos, A. J. Dahly-Vernon, K. M. Hoagland, and R. J. Roman Inhibition of the formation of EETs and 20-HETE with 1-aminobenzotriazole attenuates pressure natriuresis Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2004; 287(1): R58 - R68. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. W. Rajapakse, R. J. Roman, J. R. Falck, J. J. Oliver, and R. G. Evans Modulation of V1-receptor-mediated renal vasoconstriction by epoxyeicosatrienoic acids Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2004; 287(1): R181 - R187. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Hoagland, A. K. Flasch, and R. J. Roman Inhibitors of 20-HETE Formation Promote Salt-Sensitive Hypertension in Rats Hypertension, October 1, 2003; 42(4): 669 - 673. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Amaral, K. G. Maier, D. N. Schippers, R. J. Roman, and A. S. Greene CYP4A metabolites of arachidonic acid and VEGF are mediators of skeletal muscle angiogenesis Am J Physiol Heart Circ Physiol, May 1, 2003; 284(5): H1528 - H1535. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. E. Stec, A. Flasch, R. J. Roman, and J. A. White Distribution of cytochrome P-450 4A and 4F isoforms along the nephron in mice Am J Physiol Renal Physiol, January 1, 2003; 284(1): F95 - F102. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Xu, W. O. Straub, W. Pak, P. Su, K. G. Maier, M. Yu, R. J. Roman, P. R. Ortiz De Montellano, and D. L. Kroetz Antihypertensive effect of mechanism-based inhibition of renal arachidonic acid omega -hydroxylase activity Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2002; 283(3): R710 - R720. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
