| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan; Institute of Fisheries Science, National Taiwan University, Taipei, Taiwan
2 Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan
3 Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan; Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan
* To whom correspondence should be addressed. E-mail: pphwang{at}gate.sinica.edu.tw.
The purpose of the present work was to study the possible role of the ECaC (epithelial calcium channel) in the Ca2+ uptake mechanism in developing zebrafish (Danio rerio). Using rapid amplification of cDNA ends (RACE), full-length cDNA encoding the ECaC of zebrafish was cloned and sequenced. The cloned zECaC was 2578 base pairs in length, and encoded a protein of 709 amino acids that showed up to 73% identity with previously described vertebrate ECaCs. The zECaC was found to be expressed in all tissues examined, and began to be expressed in the skin covering the yolk sac of embryos at 24 h post-fertilization (hpf). zECaC-expressing cells expanded to cover the skin of the entire yolk sac following embryonic development, and began to occur in the gill filaments at 96 hpf, and thereafter zECaC-expressing cells rapidly increased in both gills and yolk sac skin. In correspondence to the profile of ECaC expression, the Ca2+ influx and content began to increase at 36~72 hpf. Incubating zebrafish embryos in low-Ca2+ (0.02 mM) fresh water caused upregulation of the whole-body Ca2+ influx and zECaC expression in both gills and skin. Co-localization of zECaC mRNA and the Na+/K+-ATPase 
subunit (a marker for mitochondria-rich, MR, cells) indicated that only a portion of the MR cells expressed zECaC mRNA. These results suggest that the zECaC plays a key role in Ca2+ absorption in developing zebrafish.
This article has been cited by other articles:
|
|
 |
|
  Y.-C. Tseng, R.-D. Chen, J.-R. Lee, S.-T. Liu, S.-J. Lee, and P.-P. Hwang Specific expression and regulation of glucose transporters in zebrafish ionocytes Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2009; 297(2): R275 - R290. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P.-P. Hwang Ion uptake and acid secretion in zebrafish (Danio rerio) J. Exp. Biol., June 1, 2009; 212(11): 1745 - 1752. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B.-K. Liao, R.-D. Chen, and P.-P. Hwang Expression regulation of Na+-K+-ATPase {alpha}1-subunit subtypes in zebrafish gill ionocytes Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2009; 296(6): R1897 - R1906. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-F. Wang, Y.-C. Tseng, J.-J. Yan, J. Hiroi, and P.-P. Hwang Role of SLC12A10.2, a Na-Cl cotransporter-like protein, in a Cl uptake mechanism in zebrafish (Danio rerio) Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2009; 296(5): R1650 - R1660. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Inokuchi, J. Hiroi, S. Watanabe, P.-P. Hwang, and T. Kaneko Morphological and functional classification of ion-absorbing mitochondria-rich cells in the gills of Mozambique tilapia J. Exp. Biol., April 1, 2009; 212(7): 1003 - 1010. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-L. Horng, L.-Y. Lin, and P.-P. Hwang Functional regulation of H+-ATPase-rich cells in zebrafish embryos acclimated to an acidic environment Am J Physiol Cell Physiol, April 1, 2009; 296(4): C682 - C692. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.-J. Chang, J.-L. Horng, J.-J. Yan, C.-D. Hsiao, and P.-P. Hwang The transcription factor, glial cell missing 2, is involved in differentiation and functional regulation of H+-ATPase-rich cells in zebrafish (Danio rerio) Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2009; 296(4): R1192 - R1201. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Cruz, J.-C. Shiao, B.-K. Liao, C.-J. Huang, and P.-P. Hwang Plasma membrane calcium ATPase required for semicircular canal formation and otolith growth in the zebrafish inner ear J. Exp. Biol., March 1, 2009; 212(5): 639 - 647. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D.-Y. Tseng, M.-Y. Chou, Y.-C. Tseng, C.-D. Hsiao, C.-J. Huang, T. Kaneko, and P.-P. Hwang Effects of stanniocalcin 1 on calcium uptake in zebrafish (Danio rerio) embryo Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2009; 296(3): R549 - R557. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Hiroi, S. Yasumasu, S. D. McCormick, P.-P. Hwang, and T. Kaneko Evidence for an apical Na-Cl cotransporter involved in ion uptake in a teleost fish J. Exp. Biol., August 15, 2008; 211(16): 2584 - 2599. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Nakada, K. Hoshijima, M. Esaki, S. Nagayoshi, K. Kawakami, and S. Hirose Localization of ammonia transporter Rhcg1 in mitochondrion-rich cells of yolk sac, gill, and kidney of zebrafish and its ionic strength-dependent expression Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2007; 293(4): R1743 - R1753. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. M. Craig, C. M. Wood, and G. B. McClelland Gill membrane remodeling with soft-water acclimation in zebrafish (Danio rerio) Physiol Genomics, June 19, 2007; 30(1): 53 - 60. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Gao and M. G. Wheatly Molecular characterization of an epithelial Ca2+ channel-like gene from crayfish Procambarus clarkii J. Exp. Biol., May 15, 2007; 210(10): 1813 - 1824. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-L. Horng, L.-Y. Lin, C.-J. Huang, F. Katoh, T. Kaneko, and P.-P. Hwang Knockdown of V-ATPase subunit A (atp6v1a) impairs acid secretion and ion balance in zebrafish (Danio rerio) Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2007; 292(5): R2068 - R2076. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Esaki, K. Hoshijima, S. Kobayashi, H. Fukuda, K. Kawakami, and S. Hirose Visualization in zebrafish larvae of Na+ uptake in mitochondria-rich cells whose differentiation is dependent on foxi3a Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2007; 292(1): R470 - R480. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Shahsavarani and S. F. Perry Hormonal and environmental regulation of epithelial calcium channel in gill of rainbow trout (Oncorhynchus mykiss) Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2006; 291(5): R1490 - R1498. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-Y. Lin, J.-L. Horng, J. G. Kunkel, and P.-P. Hwang Proton pump-rich cell secretes acid in skin of zebrafish larvae Am J Physiol Cell Physiol, February 1, 2006; 290(2): C371 - C378. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
