The aim of this study was to determine the effects and mechanism of synchronized gastric electrical stimulation (SGES) on gastric contractions and gastric emptying. Methods: The first experiment was designed to study the effects of SGES on antral contractions in 4 randomized sessions. Sessions 1 (control) and 2 (atropine,) were performed in the fasting state, composed of three 30-min periods (baseline, stimulation and recovery). Sessions 3 (control) and 4 (SGES performed during 2^nd 20-min period) were performed in the fed state, consisting of two 20-min periods; glucagon was injected after the first 20 min recording. The 2^nd experiment was designed to study the effect of SGES on gastric emptying and consisted of two sessions (control and SGES). SGES was delivered with train duration of 0.5-0.8s, and a pulse frequency of 40Hz, width of 2ms and amplitude of 4mA. Results: 1) SGES induced gastric antral contractions in the fasting state. The motility index was 1.3 ± 0.5 at baseline and 6.1 ± 0.7 (P=0.001) during SGES. This excitatory effect was completely blocked by atropine. 2). SGES enhanced postprandial antral contractions impaired by glucagon. 3) SGES significantly accelerated glucagon-induced delayed gastric emptying. Gastric emptying was 25.5 ± 11.3% without SGES and 38.3 ± 10.7% with SGES (P=0.006 vs. control). Conclusions: This novel method of SGES induces gastric antral contractions in the fasting state, enhances glucagon-induced antral hypomotility in the fed state and accelerates glucagon-induced delayed gastric emptying. The effect of synchronized GES on antral contractions is mediated via the cholinergic pathway.