Conducted vasodilation may coordinate blood flow in microvascular networks during skeletal muscle contraction. We tested the hypotheses that 1) exercise training enhances conducted vasodilation and 2) age-related changes in the capacity for conduction affect muscle perfusion during contractions. To address hypothesis 1: young (4-5mo), adult (12-14mo), and old (19-21mo) C57BL6 male mice were sedentary or given access to running wheels for 8 wks. Voluntary running distances were significantly different (km/day): young 5.8±0.1, adult 3.9±0.1, old 2.2±0.1 (p<0.05). In gluteus maximus muscles, conducted vasodilation was greater in adult than young or old (p<0.05) and greater in young sedentary than old sedentary but was not affected by exercise training. Citrate synthase activity was greater with exercise training at all ages (p<0.05). mRNA for eNOS did not differ among ages but eNOS protein expression was greater in adult and old with exercise training (p<0.05). Connexin 37, 40 and 43 mRNA were not affected by exercise training and did not differ by age. To address hypothesis 2: perfusion of the gluteus maximus muscle during light to severe work loads was assessed by Doppler micro-probe at 3-26mo of age. Maximum perfusion decreased linearly across the lifespan. Perfusion at the highest work load, absolute and relative to maximum, decreased across the lifespan with a steeper decline beyond ~20 mo of age. In this model: 1) exercise training does not alter conducted vasodilation and 2) muscle perfusion is maintained up to near maximum work loads despite age-related changes in conducted vasodilation.