| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
2 Copenhagen Muscle Research Centre, August Krogh Institute, and 1 Institute of Exercise and Sports Sciences, Copenhagen, Denmark
The present study investigated the localization of ATP-sensitive K+ (KATP) channels in human skeletal muscle and the functional importance of these channels for human muscle K+ distribution at rest and during muscle activity. Membrane fractionation based on the giant vesicle technique or the sucrose-gradient technique in combination with Western blotting demonstrated that the KATP channels are mainly located in the sarcolemma. This localization was confirmed by immunohistochemical measurements. With the microdialysis technique, it was demonstrated that local application of the KATP channel inhibitor glibenclamide reduced (P < 0.05) interstitial K+ at rest from ~4.5 to 4.0 mM, whereas the concentration in the control leg remained constant. Glibenclamide had no effect on the interstitial K+ accumulation during knee-extensor exercise at a power output of 60 W. In contrast to in vitro conditions, the present study demonstrated that under in vivo conditions the KATP channels are active at rest and contribute to the accumulation of interstitial K+.
microdialysis; interstitial potassium; glibenclamide
This article has been cited by other articles:
|
|
 |
|
  D. G. Allen, G. D. Lamb, and H. Westerblad Skeletal Muscle Fatigue: Cellular Mechanisms Physiol Rev, January 1, 2008; 88(1): 287 - 332. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Zheng, A. Perianayagam, D. H. Lee, M. D. Brannan, L. E. Yang, D. Tellalian, P. Chen, K. Lemieux, A. Marette, J. H. Youn, et al. AMPK activation with AICAR provokes an acute fall in plasma [K+] Am J Physiol Cell Physiol, January 1, 2008; 294(1): C126 - C135. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Broch-Lips, K. Overgaard, H. A. Praetorius, and O. B. Nielsen Effects of extracellular HCO3 on fatigue, pHi, and K+ efflux in rat skeletal muscles J Appl Physiol, August 1, 2007; 103(2): 494 - 503. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. McKenna, I. Medved, C. A. Goodman, M. J. Brown, A. R. Bjorksten, K. T. Murphy, A. C. Petersen, S. Sostaric, and X. Gong N-acetylcysteine attenuates the decline in muscle Na+,K+-pump activity and delays fatigue during prolonged exercise in humans J. Physiol., October 1, 2006; 576(1): 279 - 288. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. G. Schrage, N. M. Dietz, and M. J. Joyner Effects of combined inhibition of ATP-sensitive potassium channels, nitric oxide, and prostaglandins on hyperemia during moderate exercise J Appl Physiol, May 1, 2006; 100(5): 1506 - 1512. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kristensen, T. Hansen, and C. Juel Membrane proteins involved in potassium shifts during muscle activity and fatigue Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2006; 290(3): R766 - R772. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Street, J.-J. Nielsen, J. Bangsbo, and C. Juel Metabolic alkalosis reduces exercise-induced acidosis and potassium accumulation in human skeletal muscle interstitium J. Physiol., July 15, 2005; 566(2): 481 - 489. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Thabet, T. Miki, S. Seino, and J.-M. Renaud Treadmill running causes significant fiber damage in skeletal muscle of KATP channel-deficient mice Physiol Genomics, July 14, 2005; 22(2): 204 - 212. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. K. Hansen, T. Clausen, and O. B. Nielsen Effects of lactic acid and catecholamines on contractility in fast-twitch muscles exposed to hyperkalemia Am J Physiol Cell Physiol, July 1, 2005; 289(1): C104 - C112. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. Gosmanov, Z. Fan, X. Mi, E. G. Schneider, and D. B. Thomason ATP-sensitive potassium channels mediate hyperosmotic stimulation of NKCC in slow-twitch muscle Am J Physiol Cell Physiol, March 1, 2004; 286(3): C586 - C595. [Abstract] [Full Text]
|
|
|
|
|
|
J. J. Nielsen, M. Mohr, C. Klarskov, M. Kristensen, P. Krustrup, C. Juel, and J. Bangsbo Effects of high-intensity intermittent training on potassium kinetics and performance in human skeletal muscle J. Physiol., February 1, 2004; 554(3): 857 - 870. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. H. Pedersen, T. Clausen, and O. B. Nielsen Loss of force induced by high extracellular [K+] in rat muscle: effect of temperature, lactic acid and {beta}2-agonist J. Physiol., August 15, 2003; 551(1): 277 - 286. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
