AJP - Regu Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Regul Integr Comp Physiol 284: R1219-R1230, 2003. First published January 23, 2003; doi:10.1152/ajpregu.00257.2002
0363-6119/03 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
284/5/R1219    most recent
00257.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (14)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yuan, B.
Right arrow Articles by Cowley, A. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yuan, B.
Right arrow Articles by Cowley, A. W., Jr.
Vol. 284, Issue 5, R1219-R1230, May 2003

Gene expression reveals vulnerability to oxidative stress and interstitial fibrosis of renal outer medulla to nonhypertensive elevations of ANG II

Baozhi Yuan, Mingyu Liang, Zhizhang Yang, Elizabeth Rute, Norman Taylor, Michael Olivier, and Allen W. Cowley Jr.

Department of Physiology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226

The present study was designed to determine whether nonhypertensive elevations of plasma ANG II would modify the expression of genes involved in renal injury that could influence oxidative stress and extracellular matrix formation in the renal medulla using microarray, Northern, and Western blot techniques. Sprague-Dawley rats were infused intravenously with either ANG II (5 ng · kg-1 · min-1) or vehicle for 7 days (n = 6/group). Mean arterial pressure averaged 110 ± 0.6 mmHg during the control period and 113 ± 0.4 mmHg after ANG II. The mRNA of 1,751 genes (~80% of all currently known rat genes) that was differentially expressed (ANG II vs. saline) in renal outer and inner medulla was determined. The results of 12 hybridizations indicated that in response to ANG II, 11 genes were upregulated and 25 were downregulated in the outer medulla, while 11 were upregulated and 13 were downregulated in the inner medulla. These differentially expressed genes, most of which were not known previously to be affected by ANG II in the renal medulla, were found to group into eight physiological pathways known to influence renal injury and kidney function. Particularly, expression of several genes would be expected to increase oxidative stress and interstitial fibrosis in the outer medulla. Western blot analyses confirmed increased expression of transforming growth factor-beta 1 and collagen type IV proteins in the outer medulla. Results demonstrate that nonhypertensive elevations of plasma ANG II can significantly alter the expression of a variety of genes in the renal outer medulla and suggested the vulnerability of the renal outer medulla to the injurious effect of ANG II.

renal inner medulla; cDNA microarray


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
K. D. Pendergrass, D. B. Averill, C. M. Ferrario, D. I. Diz, and M. C. Chappell
Differential expression of nuclear AT1 receptors and angiotensin II within the kidney of the male congenic mRen2.Lewis rat
Am J Physiol Renal Physiol, June 1, 2006; 290(6): F1497 - F1506.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
J. H. Kim, J. I. Yang, M. H. Jung, J. S. Hwa, K.-R. Kang, D. J. Park, G. S. Roh, G. J. Cho, W. S. Choi, and S.-H. Chang
Heme Oxygenase-1 Protects Rat Kidney from Ureteral Obstruction via an Antiapoptotic Pathway
J. Am. Soc. Nephrol., May 1, 2006; 17(5): 1373 - 1381.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
N. Strunnikova, C. Zhang, D. Teichberg, S. W. Cousins, J. Baffi, K. G. Becker, and K. G. Csaky
Survival of Retinal Pigment Epithelium after Exposure to Prolonged Oxidative Injury: A Detailed Gene Expression and Cellular Analysis
Invest. Ophthalmol. Vis. Sci., October 1, 2004; 45(10): 3767 - 3777.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
J. Morrison, K. Knoll, M. J. Hessner, and M. Liang
Effect of high glucose on gene expression in mesangial cells: upregulation of the thiol pathway is an adaptational response
Physiol Genomics, May 19, 2004; 17(3): 271 - 282.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
M. Liang, A. W. Cowley Jr, and A. S. Greene
High throughput gene expression profiling: a molecular approach to integrative physiology
J. Physiol., January 1, 2004; 554(1): 22 - 30.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
F. Schwartz, A. Duka, E. Triantafyllidi, C. Johns, I. Duka, J. Cui, and H. Gavras
Serial analysis of gene expression in mouse kidney following angiotensin II administration
Physiol Genomics, December 16, 2003; 16(1): 90 - 98.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
J. B. Pritchard
The gill and homeostasis: transport under stress
Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2003; 285(6): R1269 - R1271.
[Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
M. Liang, A. G. Briggs, E. Rute, A. S. Greene, and A. W. Cowley Jr.
Quantitative assessment of the importance of dye switching and biological replication in cDNA microarray studies
Physiol Genomics, August 15, 2003; 14(3): 199 - 207.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online